Bismark report for: /gscratch/srlab/sr320/data/caligus/Sealice_F1_S20_L001_R1_001_val_1_val_1.fq.gz and /gscratch/srlab/sr320/data/caligus/Sealice_F1_S20_L001_R2_001_val_2_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 112799336 Number of paired-end alignments with a unique best hit: 532093 Mapping efficiency: 0.5% Sequence pairs with no alignments under any condition: 112048372 Sequence pairs did not map uniquely: 218871 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 270168 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 261925 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 6792462 Total methylated C's in CpG context: 44803 Total methylated C's in CHG context: 37016 Total methylated C's in CHH context: 2099417 Total methylated C's in Unknown context: 32004 Total unmethylated C's in CpG context: 376529 Total unmethylated C's in CHG context: 446628 Total unmethylated C's in CHH context: 3788069 Total unmethylated C's in Unknown context: 47091 C methylated in CpG context: 10.6% C methylated in CHG context: 7.7% C methylated in CHH context: 35.7% C methylated in unknown context (CN or CHN): 40.5% Bismark completed in 0d 3h 39m 20s