Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/caligus/Sealice_F1_S20_L001_R1_001_val_1_val_1.fq.gz and /gscratch/srlab/sr320/data/caligus/Sealice_F1_S20_L001_R2_001_val_2_val_2.fq.gz

Data processed at 15:59 on 2019-09-23


Alignment Stats

Sequence pairs analysed in total 112799336
Paired-end alignments with a unique best hit 532093
Pairs without alignments under any condition 112048372
Pairs that did not map uniquely 218871
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 6792462
Methylated C's in CpG context 44803
Methylated C's in CHG context 37016
Methylated C's in CHH context 2099417
Methylated C's in Unknown context 32004
Unmethylated C's in CpG context 376529
Unmethylated C's in CHG context 446628
Unmethylated C's in CHH context 3788069
Unmethylated C's in Unknown context 47091
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 7.7%
Percentage methylation (CHH context) 35.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 270168 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 261925 original bottom strand

Deduplication

Alignments analysed 532093
Unique alignments 354439
Duplicates removed 177654
Duplicated alignments were found at 90304 different positions

Cytosine Methylation after Extraction

Total C's analysed 2140924
Methylated C's in CpG context 11363
Methylated C's in CHG context 14516
Methylated C's in CHH context 739353
Unmethylated C's in CpG context 80596
Unmethylated C's in CHG context 117172
Unmethylated C's in CHH context 1177924
Percentage methylation (CpG context) 12.4%
Percentage methylation (CHG context) 11.0%
Percentage methylation (CHH context) 38.6%

M-Bias Plot