Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_all_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 605809374
Single-end alignments with a unique best hit 713
Sequences without alignments under any condition 605808661
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 5561
Methylated C's in CpG context 187
Methylated C's in CHG context 121
Methylated C's in CHH context 1287
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 197
Unmethylated C's in CHG context 224
Unmethylated C's in CHH context 3545
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 48.7%
Percentage methylation (CHG context) 35.1%
Percentage methylation (CHH context) 26.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 101 original top strand
CTOT 220 complementary to original top strand
CTOB 253 complementary to original bottom strand
OB 139 original bottom strand

Deduplication

Alignments analysed 713
Unique alignments 306
Duplicates removed 407
Duplicated alignments were found at 79 different positions

Cytosine Methylation after Extraction

Total C's analysed 2096
Methylated C's in CpG context 142
Methylated C's in CHG context 107
Methylated C's in CHH context 580
Unmethylated C's in CpG context 96
Unmethylated C's in CHG context 144
Unmethylated C's in CHH context 1027
Percentage methylation (CpG context) 59.7%
Percentage methylation (CHG context) 42.6%
Percentage methylation (CHH context) 36.1%

M-Bias Plot