Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_9_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 77350922
Single-end alignments with a unique best hit 74
Sequences without alignments under any condition 77350848
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 604
Methylated C's in CpG context 22
Methylated C's in CHG context 16
Methylated C's in CHH context 124
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 30
Unmethylated C's in CHG context 24
Unmethylated C's in CHH context 388
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 42.3%
Percentage methylation (CHG context) 40.0%
Percentage methylation (CHH context) 24.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15 original top strand
CTOT 21 complementary to original top strand
CTOB 22 complementary to original bottom strand
OB 16 original bottom strand

Deduplication

Alignments analysed 74
Unique alignments 58
Duplicates removed 16
Duplicated alignments were found at 8 different positions

Cytosine Methylation after Extraction

Total C's analysed 448
Methylated C's in CpG context 19
Methylated C's in CHG context 15
Methylated C's in CHH context 95
Unmethylated C's in CpG context 30
Unmethylated C's in CHG context 21
Unmethylated C's in CHH context 268
Percentage methylation (CpG context) 38.8%
Percentage methylation (CHG context) 41.7%
Percentage methylation (CHH context) 26.2%

M-Bias Plot