Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_8_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 38697189
Single-end alignments with a unique best hit 33
Sequences without alignments under any condition 38697156
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 244
Methylated C's in CpG context 8
Methylated C's in CHG context 8
Methylated C's in CHH context 51
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 5
Unmethylated C's in CHG context 10
Unmethylated C's in CHH context 162
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 61.5%
Percentage methylation (CHG context) 44.4%
Percentage methylation (CHH context) 23.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 9 original top strand
CTOT 6 complementary to original top strand
CTOB 13 complementary to original bottom strand
OB 5 original bottom strand

Deduplication

Alignments analysed 33
Unique alignments 28
Duplicates removed 5
Duplicated alignments were found at 4 different positions

Cytosine Methylation after Extraction

Total C's analysed 195
Methylated C's in CpG context 8
Methylated C's in CHG context 7
Methylated C's in CHH context 45
Unmethylated C's in CpG context 5
Unmethylated C's in CHG context 9
Unmethylated C's in CHH context 121
Percentage methylation (CpG context) 61.5%
Percentage methylation (CHG context) 43.8%
Percentage methylation (CHH context) 27.1%

M-Bias Plot