Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_7_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 49730113
Single-end alignments with a unique best hit 52
Sequences without alignments under any condition 49730061
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0
0.000105%100%0%0%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 398
Methylated C's in CpG context 9
Methylated C's in CHG context 6
Methylated C's in CHH context 84
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 20
Unmethylated C's in CHG context 17
Unmethylated C's in CHH context 262
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 31.0%
Percentage methylation (CHG context) 26.1%
Percentage methylation (CHH context) 24.3%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 13 original top strand
CTOT 11 complementary to original top strand
CTOB 13 complementary to original bottom strand
OB 15 original bottom strand
OTCTOTCTOBOB02468101214
Number of Alignments

Deduplication

Alignments analysed 52
Unique alignments 43
Duplicates removed 9
Duplicated alignments were found at 7 different positions
82.7%17.3%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 320
Methylated C's in CpG context 9
Methylated C's in CHG context 6
Methylated C's in CHH context 71
Unmethylated C's in CpG context 16
Unmethylated C's in CHG context 13
Unmethylated C's in CHH context 205
Percentage methylation (CpG context) 36.0%
Percentage methylation (CHG context) 31.6%
Percentage methylation (CHH context) 25.7%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

51015202530020406080100051015
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls