Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_6_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 46455148
Single-end alignments with a unique best hit 49
Sequences without alignments under any condition 46455099
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 386
Methylated C's in CpG context 26
Methylated C's in CHG context 20
Methylated C's in CHH context 110
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 13
Unmethylated C's in CHG context 15
Unmethylated C's in CHH context 202
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 66.7%
Percentage methylation (CHG context) 57.1%
Percentage methylation (CHH context) 35.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5 original top strand
CTOT 12 complementary to original top strand
CTOB 21 complementary to original bottom strand
OB 11 original bottom strand

Deduplication

Alignments analysed 49
Unique alignments 39
Duplicates removed 10
Duplicated alignments were found at 5 different positions

Cytosine Methylation after Extraction

Total C's analysed 289
Methylated C's in CpG context 26
Methylated C's in CHG context 19
Methylated C's in CHH context 82
Unmethylated C's in CpG context 13
Unmethylated C's in CHG context 14
Unmethylated C's in CHH context 135
Percentage methylation (CpG context) 66.7%
Percentage methylation (CHG context) 57.6%
Percentage methylation (CHH context) 37.8%

M-Bias Plot