Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 47401736
Single-end alignments with a unique best hit 64
Sequences without alignments under any condition 47401672
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 497
Methylated C's in CpG context 21
Methylated C's in CHG context 25
Methylated C's in CHH context 156
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 18
Unmethylated C's in CHG context 21
Unmethylated C's in CHH context 256
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 53.8%
Percentage methylation (CHG context) 54.3%
Percentage methylation (CHH context) 37.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8 original top strand
CTOT 14 complementary to original top strand
CTOB 30 complementary to original bottom strand
OB 12 original bottom strand

Deduplication

Alignments analysed 64
Unique alignments 53
Duplicates removed 11
Duplicated alignments were found at 6 different positions

Cytosine Methylation after Extraction

Total C's analysed 394
Methylated C's in CpG context 21
Methylated C's in CHG context 24
Methylated C's in CHH context 133
Unmethylated C's in CpG context 12
Unmethylated C's in CHG context 15
Unmethylated C's in CHH context 189
Percentage methylation (CpG context) 63.6%
Percentage methylation (CHG context) 61.5%
Percentage methylation (CHH context) 41.3%

M-Bias Plot