Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 44905078
Single-end alignments with a unique best hit 70
Sequences without alignments under any condition 44905008
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 562
Methylated C's in CpG context 12
Methylated C's in CHG context 13
Methylated C's in CHH context 123
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 21
Unmethylated C's in CHG context 19
Unmethylated C's in CHH context 374
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 36.4%
Percentage methylation (CHG context) 40.6%
Percentage methylation (CHH context) 24.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 17 original top strand
CTOT 19 complementary to original top strand
CTOB 25 complementary to original bottom strand
OB 9 original bottom strand

Deduplication

Alignments analysed 70
Unique alignments 47
Duplicates removed 23
Duplicated alignments were found at 10 different positions

Cytosine Methylation after Extraction

Total C's analysed 347
Methylated C's in CpG context 12
Methylated C's in CHG context 9
Methylated C's in CHH context 80
Unmethylated C's in CpG context 19
Unmethylated C's in CHG context 14
Unmethylated C's in CHH context 213
Percentage methylation (CpG context) 38.7%
Percentage methylation (CHG context) 39.1%
Percentage methylation (CHH context) 27.3%

M-Bias Plot