Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_3_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 48376729
Single-end alignments with a unique best hit 59
Sequences without alignments under any condition 48376670
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 444
Methylated C's in CpG context 15
Methylated C's in CHG context 22
Methylated C's in CHH context 118
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 27
Unmethylated C's in CHG context 19
Unmethylated C's in CHH context 243
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 35.7%
Percentage methylation (CHG context) 53.7%
Percentage methylation (CHH context) 32.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8 original top strand
CTOT 23 complementary to original top strand
CTOB 13 complementary to original bottom strand
OB 15 original bottom strand

Deduplication

Alignments analysed 59
Unique alignments 52
Duplicates removed 7
Duplicated alignments were found at 4 different positions

Cytosine Methylation after Extraction

Total C's analysed 378
Methylated C's in CpG context 14
Methylated C's in CHG context 22
Methylated C's in CHH context 111
Unmethylated C's in CpG context 24
Unmethylated C's in CHG context 17
Unmethylated C's in CHH context 190
Percentage methylation (CpG context) 36.8%
Percentage methylation (CHG context) 56.4%
Percentage methylation (CHH context) 36.9%

M-Bias Plot