Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 46105140
Single-end alignments with a unique best hit 55
Sequences without alignments under any condition 46105085
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0
0.000119%100%0%0%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 395
Methylated C's in CpG context 19
Methylated C's in CHG context 17
Methylated C's in CHH context 114
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 20
Unmethylated C's in CHG context 16
Unmethylated C's in CHH context 209
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 48.7%
Percentage methylation (CHG context) 51.5%
Percentage methylation (CHH context) 35.3%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 12 original top strand
CTOT 14 complementary to original top strand
CTOB 17 complementary to original bottom strand
OB 12 original bottom strand
OTCTOTCTOBOB0246810121416
Number of Alignments

Deduplication

Alignments analysed 55
Unique alignments 48
Duplicates removed 7
Duplicated alignments were found at 4 different positions
87.3%12.7%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 343
Methylated C's in CpG context 19
Methylated C's in CHG context 17
Methylated C's in CHH context 98
Unmethylated C's in CpG context 20
Unmethylated C's in CHG context 16
Unmethylated C's in CHH context 173
Percentage methylation (CpG context) 48.7%
Percentage methylation (CHG context) 51.5%
Percentage methylation (CHH context) 36.2%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

510152025300204060801000246810121416
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls