Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_1_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 46792286
Single-end alignments with a unique best hit 51
Sequences without alignments under any condition 46792235
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 404
Methylated C's in CpG context 13
Methylated C's in CHG context 10
Methylated C's in CHH context 97
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 25
Unmethylated C's in CHG context 32
Unmethylated C's in CHH context 227
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 34.2%
Percentage methylation (CHG context) 23.8%
Percentage methylation (CHH context) 29.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6 original top strand
CTOT 21 complementary to original top strand
CTOB 17 complementary to original bottom strand
OB 7 original bottom strand

Deduplication

Alignments analysed 51
Unique alignments 38
Duplicates removed 13
Duplicated alignments were found at 8 different positions

Cytosine Methylation after Extraction

Total C's analysed 285
Methylated C's in CpG context 11
Methylated C's in CHG context 10
Methylated C's in CHH context 66
Unmethylated C's in CpG context 22
Unmethylated C's in CHG context 30
Unmethylated C's in CHH context 146
Percentage methylation (CpG context) 33.3%
Percentage methylation (CHG context) 25.0%
Percentage methylation (CHH context) 31.1%

M-Bias Plot