Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 55048106
Single-end alignments with a unique best hit 53
Sequences without alignments under any condition 55048053
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 411
Methylated C's in CpG context 11
Methylated C's in CHG context 10
Methylated C's in CHH context 98
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 11
Unmethylated C's in CHG context 15
Unmethylated C's in CHH context 266
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 50.0%
Percentage methylation (CHG context) 40.0%
Percentage methylation (CHH context) 26.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7 original top strand
CTOT 16 complementary to original top strand
CTOB 19 complementary to original bottom strand
OB 11 original bottom strand

Deduplication

Alignments analysed 53
Unique alignments 42
Duplicates removed 11
Duplicated alignments were found at 7 different positions

Cytosine Methylation after Extraction

Total C's analysed 308
Methylated C's in CpG context 11
Methylated C's in CHG context 10
Methylated C's in CHH context 85
Unmethylated C's in CpG context 11
Unmethylated C's in CHG context 15
Unmethylated C's in CHH context 176
Percentage methylation (CpG context) 50.0%
Percentage methylation (CHG context) 40.0%
Percentage methylation (CHH context) 32.6%

M-Bias Plot