Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_17_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 42070546
Single-end alignments with a unique best hit 58
Sequences without alignments under any condition 42070488
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 415
Methylated C's in CpG context 12
Methylated C's in CHG context 7
Methylated C's in CHH context 98
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 9
Unmethylated C's in CHG context 16
Unmethylated C's in CHH context 273
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 57.1%
Percentage methylation (CHG context) 30.4%
Percentage methylation (CHH context) 26.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 9 original top strand
CTOT 12 complementary to original top strand
CTOB 21 complementary to original bottom strand
OB 16 original bottom strand

Deduplication

Alignments analysed 58
Unique alignments 42
Duplicates removed 16
Duplicated alignments were found at 5 different positions

Cytosine Methylation after Extraction

Total C's analysed 280
Methylated C's in CpG context 12
Methylated C's in CHG context 7
Methylated C's in CHH context 67
Unmethylated C's in CpG context 8
Unmethylated C's in CHG context 13
Unmethylated C's in CHH context 173
Percentage methylation (CpG context) 60.0%
Percentage methylation (CHG context) 35.0%
Percentage methylation (CHH context) 27.9%

M-Bias Plot