Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_16_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/phiX174/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 74531056 Number of alignments with a unique best hit from the different alignments: 99 Mapping efficiency: 0.0% Sequences with no alignments under any condition: 74530957 Sequences did not map uniquely: 0 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 12 ((converted) top strand) CT/GA: 18 ((converted) bottom strand) GA/CT: 39 (complementary to (converted) top strand) GA/GA: 30 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 800 Total methylated C's in CpG context: 24 Total methylated C's in CHG context: 27 Total methylated C's in CHH context: 204 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 21 Total unmethylated C's in CHG context: 25 Total unmethylated C's in CHH context: 499 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 53.3% C methylated in CHG context: 51.9% C methylated in CHH context: 29.0% Can't determine percentage of methylated Cs in Unknown context (CN or CHN) if value was 0 Bismark completed in 0d 0h 28m 58s