Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_16_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 74531056
Single-end alignments with a unique best hit 99
Sequences without alignments under any condition 74530957
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 800
Methylated C's in CpG context 24
Methylated C's in CHG context 27
Methylated C's in CHH context 204
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 21
Unmethylated C's in CHG context 25
Unmethylated C's in CHH context 499
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 53.3%
Percentage methylation (CHG context) 51.9%
Percentage methylation (CHH context) 29.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 12 original top strand
CTOT 39 complementary to original top strand
CTOB 30 complementary to original bottom strand
OB 18 original bottom strand

Deduplication

Alignments analysed 99
Unique alignments 60
Duplicates removed 39
Duplicated alignments were found at 13 different positions

Cytosine Methylation after Extraction

Total C's analysed 442
Methylated C's in CpG context 24
Methylated C's in CHG context 25
Methylated C's in CHH context 134
Unmethylated C's in CpG context 15
Unmethylated C's in CHG context 19
Unmethylated C's in CHH context 225
Percentage methylation (CpG context) 61.5%
Percentage methylation (CHG context) 56.8%
Percentage methylation (CHH context) 37.3%

M-Bias Plot