Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_15_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 67984205
Single-end alignments with a unique best hit 92
Sequences without alignments under any condition 67984113
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 734
Methylated C's in CpG context 18
Methylated C's in CHG context 9
Methylated C's in CHH context 167
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 25
Unmethylated C's in CHG context 30
Unmethylated C's in CHH context 485
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 41.9%
Percentage methylation (CHG context) 23.1%
Percentage methylation (CHH context) 25.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 13 original top strand
CTOT 26 complementary to original top strand
CTOB 32 complementary to original bottom strand
OB 21 original bottom strand

Deduplication

Alignments analysed 92
Unique alignments 55
Duplicates removed 37
Duplicated alignments were found at 9 different positions

Cytosine Methylation after Extraction

Total C's analysed 397
Methylated C's in CpG context 16
Methylated C's in CHG context 9
Methylated C's in CHH context 108
Unmethylated C's in CpG context 22
Unmethylated C's in CHG context 23
Unmethylated C's in CHH context 219
Percentage methylation (CpG context) 42.1%
Percentage methylation (CHG context) 28.1%
Percentage methylation (CHH context) 33.0%

M-Bias Plot