Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 87834637
Single-end alignments with a unique best hit 96
Sequences without alignments under any condition 87834541
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 809
Methylated C's in CpG context 27
Methylated C's in CHG context 16
Methylated C's in CHH context 153
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 30
Unmethylated C's in CHG context 28
Unmethylated C's in CHH context 555
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 47.4%
Percentage methylation (CHG context) 36.4%
Percentage methylation (CHH context) 21.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14 original top strand
CTOT 37 complementary to original top strand
CTOB 28 complementary to original bottom strand
OB 17 original bottom strand

Deduplication

Alignments analysed 96
Unique alignments 55
Duplicates removed 41
Duplicated alignments were found at 11 different positions

Cytosine Methylation after Extraction

Total C's analysed 417
Methylated C's in CpG context 24
Methylated C's in CHG context 15
Methylated C's in CHH context 93
Unmethylated C's in CpG context 24
Unmethylated C's in CHG context 25
Unmethylated C's in CHH context 236
Percentage methylation (CpG context) 50.0%
Percentage methylation (CHG context) 37.5%
Percentage methylation (CHH context) 28.3%

M-Bias Plot