Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 64352550
Single-end alignments with a unique best hit 66
Sequences without alignments under any condition 64352484
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 513
Methylated C's in CpG context 17
Methylated C's in CHG context 8
Methylated C's in CHH context 126
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 22
Unmethylated C's in CHG context 23
Unmethylated C's in CHH context 317
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 43.6%
Percentage methylation (CHG context) 25.8%
Percentage methylation (CHH context) 28.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 9 original top strand
CTOT 20 complementary to original top strand
CTOB 28 complementary to original bottom strand
OB 9 original bottom strand

Deduplication

Alignments analysed 66
Unique alignments 44
Duplicates removed 22
Duplicated alignments were found at 12 different positions

Cytosine Methylation after Extraction

Total C's analysed 326
Methylated C's in CpG context 16
Methylated C's in CHG context 8
Methylated C's in CHH context 95
Unmethylated C's in CpG context 12
Unmethylated C's in CHG context 16
Unmethylated C's in CHH context 179
Percentage methylation (CpG context) 57.1%
Percentage methylation (CHG context) 33.3%
Percentage methylation (CHH context) 34.7%

M-Bias Plot