Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 59459431
Single-end alignments with a unique best hit 56
Sequences without alignments under any condition 59459375
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 406
Methylated C's in CpG context 16
Methylated C's in CHG context 6
Methylated C's in CHH context 99
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 12
Unmethylated C's in CHG context 18
Unmethylated C's in CHH context 255
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 57.1%
Percentage methylation (CHG context) 25.0%
Percentage methylation (CHH context) 28.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6 original top strand
CTOT 12 complementary to original top strand
CTOB 24 complementary to original bottom strand
OB 14 original bottom strand

Deduplication

Alignments analysed 56
Unique alignments 45
Duplicates removed 11
Duplicated alignments were found at 9 different positions

Cytosine Methylation after Extraction

Total C's analysed 323
Methylated C's in CpG context 13
Methylated C's in CHG context 6
Methylated C's in CHH context 75
Unmethylated C's in CpG context 12
Unmethylated C's in CHG context 17
Unmethylated C's in CHH context 200
Percentage methylation (CpG context) 52.0%
Percentage methylation (CHG context) 26.1%
Percentage methylation (CHH context) 27.3%

M-Bias Plot