Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_11_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 54458572
Single-end alignments with a unique best hit 68
Sequences without alignments under any condition 54458504
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 553
Methylated C's in CpG context 14
Methylated C's in CHG context 8
Methylated C's in CHH context 97
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 20
Unmethylated C's in CHG context 25
Unmethylated C's in CHH context 389
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 41.2%
Percentage methylation (CHG context) 24.2%
Percentage methylation (CHH context) 20.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14 original top strand
CTOT 11 complementary to original top strand
CTOB 28 complementary to original bottom strand
OB 15 original bottom strand

Deduplication

Alignments analysed 68
Unique alignments 41
Duplicates removed 27
Duplicated alignments were found at 10 different positions

Cytosine Methylation after Extraction

Total C's analysed 311
Methylated C's in CpG context 10
Methylated C's in CHG context 7
Methylated C's in CHH context 64
Unmethylated C's in CpG context 17
Unmethylated C's in CHG context 19
Unmethylated C's in CHH context 194
Percentage methylation (CpG context) 37.0%
Percentage methylation (CHG context) 26.9%
Percentage methylation (CHH context) 24.8%

M-Bias Plot