Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_10_s456_trimmed.fq

Data processed at 22:02 on 2022-09-14


Alignment Stats

Sequences analysed in total 78221457
Single-end alignments with a unique best hit 100
Sequences without alignments under any condition 78221357
Sequences that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 722
Methylated C's in CpG context 40
Methylated C's in CHG context 25
Methylated C's in CHH context 190
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 42
Unmethylated C's in CHG context 32
Unmethylated C's in CHH context 393
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 48.8%
Percentage methylation (CHG context) 43.9%
Percentage methylation (CHH context) 32.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 16 original top strand
CTOT 34 complementary to original top strand
CTOB 35 complementary to original bottom strand
OB 15 original bottom strand

Deduplication

Alignments analysed 100
Unique alignments 64
Duplicates removed 36
Duplicated alignments were found at 19 different positions

Cytosine Methylation after Extraction

Total C's analysed 442
Methylated C's in CpG context 33
Methylated C's in CHG context 22
Methylated C's in CHH context 130
Unmethylated C's in CpG context 24
Unmethylated C's in CHG context 23
Unmethylated C's in CHH context 210
Percentage methylation (CpG context) 57.9%
Percentage methylation (CHG context) 48.9%
Percentage methylation (CHH context) 38.2%

M-Bias Plot