Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_all_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 605809374
Single-end alignments with a unique best hit 7659
Sequences without alignments under any condition 605801586
Sequences that did not map uniquely 129
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 56435
Methylated C's in CpG context 4281
Methylated C's in CHG context 995
Methylated C's in CHH context 7649
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 3045
Unmethylated C's in CHG context 5999
Unmethylated C's in CHH context 34466
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 58.4%
Percentage methylation (CHG context) 14.2%
Percentage methylation (CHH context) 18.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1008 original top strand
CTOT 1303 complementary to original top strand
CTOB 3078 complementary to original bottom strand
OB 2270 original bottom strand

Deduplication

Alignments analysed 7659
Unique alignments 2424
Duplicates removed 5235
Duplicated alignments were found at 788 different positions

Cytosine Methylation after Extraction

Total C's analysed 16875
Methylated C's in CpG context 782
Methylated C's in CHG context 404
Methylated C's in CHH context 3040
Unmethylated C's in CpG context 1132
Unmethylated C's in CHG context 1965
Unmethylated C's in CHH context 9552
Percentage methylation (CpG context) 40.9%
Percentage methylation (CHG context) 17.1%
Percentage methylation (CHH context) 24.1%

M-Bias Plot