Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_9_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 77350922
Single-end alignments with a unique best hit 843
Sequences without alignments under any condition 77350061
Sequences that did not map uniquely 18
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 5974
Methylated C's in CpG context 307
Methylated C's in CHG context 105
Methylated C's in CHH context 924
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 368
Unmethylated C's in CHG context 609
Unmethylated C's in CHH context 3661
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 45.5%
Percentage methylation (CHG context) 14.7%
Percentage methylation (CHH context) 20.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 144 original top strand
CTOT 152 complementary to original top strand
CTOB 311 complementary to original bottom strand
OB 236 original bottom strand

Deduplication

Alignments analysed 843
Unique alignments 504
Duplicates removed 339
Duplicated alignments were found at 117 different positions

Cytosine Methylation after Extraction

Total C's analysed 3665
Methylated C's in CpG context 134
Methylated C's in CHG context 71
Methylated C's in CHH context 647
Unmethylated C's in CpG context 248
Unmethylated C's in CHG context 385
Unmethylated C's in CHH context 2180
Percentage methylation (CpG context) 35.1%
Percentage methylation (CHG context) 15.6%
Percentage methylation (CHH context) 22.9%

M-Bias Plot