Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_8_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 38697189
Single-end alignments with a unique best hit 494
Sequences without alignments under any condition 38696681
Sequences that did not map uniquely 14
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 3546
Methylated C's in CpG context 212
Methylated C's in CHG context 68
Methylated C's in CHH context 553
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 198
Unmethylated C's in CHG context 330
Unmethylated C's in CHH context 2185
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 51.7%
Percentage methylation (CHG context) 17.1%
Percentage methylation (CHH context) 20.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 78 original top strand
CTOT 88 complementary to original top strand
CTOB 203 complementary to original bottom strand
OB 125 original bottom strand

Deduplication

Alignments analysed 494
Unique alignments 312
Duplicates removed 182
Duplicated alignments were found at 56 different positions

Cytosine Methylation after Extraction

Total C's analysed 2240
Methylated C's in CpG context 78
Methylated C's in CHG context 46
Methylated C's in CHH context 441
Unmethylated C's in CpG context 131
Unmethylated C's in CHG context 202
Unmethylated C's in CHH context 1342
Percentage methylation (CpG context) 37.3%
Percentage methylation (CHG context) 18.5%
Percentage methylation (CHH context) 24.7%

M-Bias Plot