Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_7_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 49730113
Single-end alignments with a unique best hit 659
Sequences without alignments under any condition 49729438
Sequences that did not map uniquely 16
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4800
Methylated C's in CpG context 311
Methylated C's in CHG context 71
Methylated C's in CHH context 670
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 317
Unmethylated C's in CHG context 538
Unmethylated C's in CHH context 2893
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 49.5%
Percentage methylation (CHG context) 11.7%
Percentage methylation (CHH context) 18.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 116 original top strand
CTOT 94 complementary to original top strand
CTOB 262 complementary to original bottom strand
OB 187 original bottom strand

Deduplication

Alignments analysed 659
Unique alignments 390
Duplicates removed 269
Duplicated alignments were found at 72 different positions

Cytosine Methylation after Extraction

Total C's analysed 2860
Methylated C's in CpG context 134
Methylated C's in CHG context 50
Methylated C's in CHH context 467
Unmethylated C's in CpG context 201
Unmethylated C's in CHG context 336
Unmethylated C's in CHH context 1672
Percentage methylation (CpG context) 40.0%
Percentage methylation (CHG context) 13.0%
Percentage methylation (CHH context) 21.8%

M-Bias Plot