Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_6_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 46455148
Single-end alignments with a unique best hit 607
Sequences without alignments under any condition 46454530
Sequences that did not map uniquely 11
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4368
Methylated C's in CpG context 220
Methylated C's in CHG context 123
Methylated C's in CHH context 672
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 301
Unmethylated C's in CHG context 453
Unmethylated C's in CHH context 2599
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 42.2%
Percentage methylation (CHG context) 21.4%
Percentage methylation (CHH context) 20.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 119 original top strand
CTOT 98 complementary to original top strand
CTOB 220 complementary to original bottom strand
OB 170 original bottom strand

Deduplication

Alignments analysed 607
Unique alignments 372
Duplicates removed 235
Duplicated alignments were found at 83 different positions

Cytosine Methylation after Extraction

Total C's analysed 2748
Methylated C's in CpG context 105
Methylated C's in CHG context 60
Methylated C's in CHH context 450
Unmethylated C's in CpG context 186
Unmethylated C's in CHG context 319
Unmethylated C's in CHH context 1628
Percentage methylation (CpG context) 36.1%
Percentage methylation (CHG context) 15.8%
Percentage methylation (CHH context) 21.7%

M-Bias Plot