Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 47401736
Single-end alignments with a unique best hit 607
Sequences without alignments under any condition 47401115
Sequences that did not map uniquely 14
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4306
Methylated C's in CpG context 204
Methylated C's in CHG context 100
Methylated C's in CHH context 733
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 275
Unmethylated C's in CHG context 440
Unmethylated C's in CHH context 2554
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 42.6%
Percentage methylation (CHG context) 18.5%
Percentage methylation (CHH context) 22.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 110 original top strand
CTOT 113 complementary to original top strand
CTOB 232 complementary to original bottom strand
OB 152 original bottom strand

Deduplication

Alignments analysed 607
Unique alignments 395
Duplicates removed 212
Duplicated alignments were found at 81 different positions

Cytosine Methylation after Extraction

Total C's analysed 2833
Methylated C's in CpG context 102
Methylated C's in CHG context 60
Methylated C's in CHH context 538
Unmethylated C's in CpG context 177
Unmethylated C's in CHG context 300
Unmethylated C's in CHH context 1656
Percentage methylation (CpG context) 36.6%
Percentage methylation (CHG context) 16.7%
Percentage methylation (CHH context) 24.5%

M-Bias Plot