Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 44905078
Single-end alignments with a unique best hit 564
Sequences without alignments under any condition 44904503
Sequences that did not map uniquely 11
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4255
Methylated C's in CpG context 309
Methylated C's in CHG context 105
Methylated C's in CHH context 635
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 266
Unmethylated C's in CHG context 434
Unmethylated C's in CHH context 2506
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 53.7%
Percentage methylation (CHG context) 19.5%
Percentage methylation (CHH context) 20.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 74 original top strand
CTOT 98 complementary to original top strand
CTOB 234 complementary to original bottom strand
OB 158 original bottom strand

Deduplication

Alignments analysed 564
Unique alignments 352
Duplicates removed 212
Duplicated alignments were found at 65 different positions

Cytosine Methylation after Extraction

Total C's analysed 2635
Methylated C's in CpG context 128
Methylated C's in CHG context 67
Methylated C's in CHH context 486
Unmethylated C's in CpG context 178
Unmethylated C's in CHG context 261
Unmethylated C's in CHH context 1515
Percentage methylation (CpG context) 41.8%
Percentage methylation (CHG context) 20.4%
Percentage methylation (CHH context) 24.3%

M-Bias Plot