Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_3_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 48376729
Single-end alignments with a unique best hit 652
Sequences without alignments under any condition 48376067
Sequences that did not map uniquely 10
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4793
Methylated C's in CpG context 271
Methylated C's in CHG context 108
Methylated C's in CHH context 734
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 281
Unmethylated C's in CHG context 494
Unmethylated C's in CHH context 2905
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 49.1%
Percentage methylation (CHG context) 17.9%
Percentage methylation (CHH context) 20.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 113 original top strand
CTOT 104 complementary to original top strand
CTOB 254 complementary to original bottom strand
OB 181 original bottom strand

Deduplication

Alignments analysed 652
Unique alignments 386
Duplicates removed 266
Duplicated alignments were found at 86 different positions

Cytosine Methylation after Extraction

Total C's analysed 2855
Methylated C's in CpG context 96
Methylated C's in CHG context 66
Methylated C's in CHH context 555
Unmethylated C's in CpG context 171
Unmethylated C's in CHG context 286
Unmethylated C's in CHH context 1681
Percentage methylation (CpG context) 36.0%
Percentage methylation (CHG context) 18.8%
Percentage methylation (CHH context) 24.8%

M-Bias Plot