Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/lambda/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 46105140 Number of alignments with a unique best hit from the different alignments: 573 Mapping efficiency: 0.0% Sequences with no alignments under any condition: 46104559 Sequences did not map uniquely: 8 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 78 ((converted) top strand) CT/GA: 160 ((converted) bottom strand) GA/CT: 103 (complementary to (converted) top strand) GA/GA: 232 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 4068 Total methylated C's in CpG context: 248 Total methylated C's in CHG context: 104 Total methylated C's in CHH context: 684 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 239 Total unmethylated C's in CHG context: 394 Total unmethylated C's in CHH context: 2399 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 50.9% C methylated in CHG context: 20.9% C methylated in CHH context: 22.2% Can't determine percentage of methylated Cs in Unknown context (CN or CHN) if value was 0 Bismark completed in 0d 0h 17m 58s