Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 46105140
Single-end alignments with a unique best hit 573
Sequences without alignments under any condition 46104559
Sequences that did not map uniquely 8
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4068
Methylated C's in CpG context 248
Methylated C's in CHG context 104
Methylated C's in CHH context 684
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 239
Unmethylated C's in CHG context 394
Unmethylated C's in CHH context 2399
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 50.9%
Percentage methylation (CHG context) 20.9%
Percentage methylation (CHH context) 22.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 78 original top strand
CTOT 103 complementary to original top strand
CTOB 232 complementary to original bottom strand
OB 160 original bottom strand

Deduplication

Alignments analysed 573
Unique alignments 352
Duplicates removed 221
Duplicated alignments were found at 79 different positions

Cytosine Methylation after Extraction

Total C's analysed 2573
Methylated C's in CpG context 128
Methylated C's in CHG context 62
Methylated C's in CHH context 495
Unmethylated C's in CpG context 154
Unmethylated C's in CHG context 254
Unmethylated C's in CHH context 1480
Percentage methylation (CpG context) 45.4%
Percentage methylation (CHG context) 19.6%
Percentage methylation (CHH context) 25.1%

M-Bias Plot