Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_1_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 46792286
Single-end alignments with a unique best hit 605
Sequences without alignments under any condition 46791676
Sequences that did not map uniquely 5
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4335
Methylated C's in CpG context 272
Methylated C's in CHG context 104
Methylated C's in CHH context 698
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 245
Unmethylated C's in CHG context 427
Unmethylated C's in CHH context 2589
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 52.6%
Percentage methylation (CHG context) 19.6%
Percentage methylation (CHH context) 21.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 109 original top strand
CTOT 105 complementary to original top strand
CTOB 220 complementary to original bottom strand
OB 171 original bottom strand

Deduplication

Alignments analysed 605
Unique alignments 399
Duplicates removed 206
Duplicated alignments were found at 77 different positions

Cytosine Methylation after Extraction

Total C's analysed 2858
Methylated C's in CpG context 126
Methylated C's in CHG context 70
Methylated C's in CHH context 567
Unmethylated C's in CpG context 162
Unmethylated C's in CHG context 286
Unmethylated C's in CHH context 1647
Percentage methylation (CpG context) 43.8%
Percentage methylation (CHG context) 19.7%
Percentage methylation (CHH context) 25.6%

M-Bias Plot