Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 55048106
Single-end alignments with a unique best hit 641
Sequences without alignments under any condition 55047455
Sequences that did not map uniquely 10
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4730
Methylated C's in CpG context 260
Methylated C's in CHG context 93
Methylated C's in CHH context 700
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 298
Unmethylated C's in CHG context 501
Unmethylated C's in CHH context 2878
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 46.6%
Percentage methylation (CHG context) 15.7%
Percentage methylation (CHH context) 19.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 93 original top strand
CTOT 110 complementary to original top strand
CTOB 267 complementary to original bottom strand
OB 171 original bottom strand

Deduplication

Alignments analysed 641
Unique alignments 386
Duplicates removed 255
Duplicated alignments were found at 82 different positions

Cytosine Methylation after Extraction

Total C's analysed 2839
Methylated C's in CpG context 104
Methylated C's in CHG context 55
Methylated C's in CHH context 509
Unmethylated C's in CpG context 185
Unmethylated C's in CHG context 294
Unmethylated C's in CHH context 1692
Percentage methylation (CpG context) 36.0%
Percentage methylation (CHG context) 15.8%
Percentage methylation (CHH context) 23.1%

M-Bias Plot