Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_17_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 42070546
Single-end alignments with a unique best hit 578
Sequences without alignments under any condition 42069954
Sequences that did not map uniquely 14
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4219
Methylated C's in CpG context 278
Methylated C's in CHG context 72
Methylated C's in CHH context 645
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 243
Unmethylated C's in CHG context 473
Unmethylated C's in CHH context 2508
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 53.4%
Percentage methylation (CHG context) 13.2%
Percentage methylation (CHH context) 20.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 70 original top strand
CTOT 106 complementary to original top strand
CTOB 243 complementary to original bottom strand
OB 159 original bottom strand

Deduplication

Alignments analysed 578
Unique alignments 368
Duplicates removed 210
Duplicated alignments were found at 70 different positions

Cytosine Methylation after Extraction

Total C's analysed 2714
Methylated C's in CpG context 117
Methylated C's in CHG context 54
Methylated C's in CHH context 510
Unmethylated C's in CpG context 173
Unmethylated C's in CHG context 309
Unmethylated C's in CHH context 1551
Percentage methylation (CpG context) 40.3%
Percentage methylation (CHG context) 14.9%
Percentage methylation (CHH context) 24.7%

M-Bias Plot