Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_16_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 74531056
Single-end alignments with a unique best hit 970
Sequences without alignments under any condition 74530066
Sequences that did not map uniquely 20
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 7182
Methylated C's in CpG context 642
Methylated C's in CHG context 97
Methylated C's in CHH context 860
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 342
Unmethylated C's in CHG context 761
Unmethylated C's in CHH context 4480
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 65.2%
Percentage methylation (CHG context) 11.3%
Percentage methylation (CHH context) 16.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 124 original top strand
CTOT 125 complementary to original top strand
CTOB 382 complementary to original bottom strand
OB 339 original bottom strand

Deduplication

Alignments analysed 970
Unique alignments 501
Duplicates removed 469
Duplicated alignments were found at 91 different positions

Cytosine Methylation after Extraction

Total C's analysed 3557
Methylated C's in CpG context 160
Methylated C's in CHG context 65
Methylated C's in CHH context 629
Unmethylated C's in CpG context 228
Unmethylated C's in CHG context 375
Unmethylated C's in CHH context 2100
Percentage methylation (CpG context) 41.2%
Percentage methylation (CHG context) 14.8%
Percentage methylation (CHH context) 23.0%

M-Bias Plot