Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_15_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 67984205
Single-end alignments with a unique best hit 928
Sequences without alignments under any condition 67983264
Sequences that did not map uniquely 13
Genomic sequence context not extractable (edges of chromosomes) 0
0.00137%100%0.0000191%0%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 7043
Methylated C's in CpG context 600
Methylated C's in CHG context 121
Methylated C's in CHH context 857
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 341
Unmethylated C's in CHG context 753
Unmethylated C's in CHH context 4371
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 63.8%
Percentage methylation (CHG context) 13.8%
Percentage methylation (CHH context) 16.4%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 114 original top strand
CTOT 152 complementary to original top strand
CTOB 385 complementary to original bottom strand
OB 277 original bottom strand
OTCTOTCTOBOB050100150200250300350400
Number of Alignments

Deduplication

Alignments analysed 928
Unique alignments 490
Duplicates removed 438
Duplicated alignments were found at 108 different positions
52.8%47.2%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 3529
Methylated C's in CpG context 164
Methylated C's in CHG context 80
Methylated C's in CHH context 597
Unmethylated C's in CpG context 212
Unmethylated C's in CHG context 401
Unmethylated C's in CHH context 2075
Percentage methylation (CpG context) 43.6%
Percentage methylation (CHG context) 16.6%
Percentage methylation (CHH context) 22.3%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

51015202530020406080100020406080100120
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls