Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 87834637
Single-end alignments with a unique best hit 1106
Sequences without alignments under any condition 87833515
Sequences that did not map uniquely 16
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 8310
Methylated C's in CpG context 686
Methylated C's in CHG context 145
Methylated C's in CHH context 1042
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 388
Unmethylated C's in CHG context 839
Unmethylated C's in CHH context 5210
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 63.9%
Percentage methylation (CHG context) 14.7%
Percentage methylation (CHH context) 16.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 155 original top strand
CTOT 167 complementary to original top strand
CTOB 442 complementary to original bottom strand
OB 342 original bottom strand

Deduplication

Alignments analysed 1106
Unique alignments 546
Duplicates removed 560
Duplicated alignments were found at 124 different positions

Cytosine Methylation after Extraction

Total C's analysed 4006
Methylated C's in CpG context 197
Methylated C's in CHG context 96
Methylated C's in CHH context 717
Unmethylated C's in CpG context 234
Unmethylated C's in CHG context 412
Unmethylated C's in CHH context 2350
Percentage methylation (CpG context) 45.7%
Percentage methylation (CHG context) 18.9%
Percentage methylation (CHH context) 23.4%

M-Bias Plot