Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 64352550
Single-end alignments with a unique best hit 842
Sequences without alignments under any condition 64351693
Sequences that did not map uniquely 15
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 6280
Methylated C's in CpG context 478
Methylated C's in CHG context 106
Methylated C's in CHH context 828
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 359
Unmethylated C's in CHG context 691
Unmethylated C's in CHH context 3818
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 57.1%
Percentage methylation (CHG context) 13.3%
Percentage methylation (CHH context) 17.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 108 original top strand
CTOT 142 complementary to original top strand
CTOB 338 complementary to original bottom strand
OB 254 original bottom strand

Deduplication

Alignments analysed 842
Unique alignments 438
Duplicates removed 404
Duplicated alignments were found at 107 different positions

Cytosine Methylation after Extraction

Total C's analysed 3236
Methylated C's in CpG context 162
Methylated C's in CHG context 64
Methylated C's in CHH context 595
Unmethylated C's in CpG context 199
Unmethylated C's in CHG context 366
Unmethylated C's in CHH context 1850
Percentage methylation (CpG context) 44.9%
Percentage methylation (CHG context) 14.9%
Percentage methylation (CHH context) 24.3%

M-Bias Plot