Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 59459431
Single-end alignments with a unique best hit 715
Sequences without alignments under any condition 59458705
Sequences that did not map uniquely 11
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 5132
Methylated C's in CpG context 266
Methylated C's in CHG context 96
Methylated C's in CHH context 849
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 309
Unmethylated C's in CHG context 522
Unmethylated C's in CHH context 3090
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 46.3%
Percentage methylation (CHG context) 15.5%
Percentage methylation (CHH context) 21.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 102 original top strand
CTOT 143 complementary to original top strand
CTOB 266 complementary to original bottom strand
OB 204 original bottom strand

Deduplication

Alignments analysed 715
Unique alignments 465
Duplicates removed 250
Duplicated alignments were found at 88 different positions

Cytosine Methylation after Extraction

Total C's analysed 3375
Methylated C's in CpG context 132
Methylated C's in CHG context 67
Methylated C's in CHH context 592
Unmethylated C's in CpG context 225
Unmethylated C's in CHG context 361
Unmethylated C's in CHH context 1998
Percentage methylation (CpG context) 37.0%
Percentage methylation (CHG context) 15.7%
Percentage methylation (CHH context) 22.9%

M-Bias Plot