Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_11_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 54458572
Single-end alignments with a unique best hit 741
Sequences without alignments under any condition 54457818
Sequences that did not map uniquely 13
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 5463
Methylated C's in CpG context 539
Methylated C's in CHG context 110
Methylated C's in CHH context 624
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 284
Unmethylated C's in CHG context 583
Unmethylated C's in CHH context 3323
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 65.5%
Percentage methylation (CHG context) 15.9%
Percentage methylation (CHH context) 15.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 93 original top strand
CTOT 114 complementary to original top strand
CTOB 281 complementary to original bottom strand
OB 253 original bottom strand

Deduplication

Alignments analysed 741
Unique alignments 382
Duplicates removed 359
Duplicated alignments were found at 72 different positions

Cytosine Methylation after Extraction

Total C's analysed 2748
Methylated C's in CpG context 152
Methylated C's in CHG context 79
Methylated C's in CHH context 458
Unmethylated C's in CpG context 177
Unmethylated C's in CHG context 305
Unmethylated C's in CHH context 1577
Percentage methylation (CpG context) 46.2%
Percentage methylation (CHG context) 20.6%
Percentage methylation (CHH context) 22.5%

M-Bias Plot