Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_10_s456_trimmed.fq

Data processed at 21:14 on 2022-09-14


Alignment Stats

Sequences analysed in total 78221457
Single-end alignments with a unique best hit 858
Sequences without alignments under any condition 78220584
Sequences that did not map uniquely 15
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 6062
Methylated C's in CpG context 411
Methylated C's in CHG context 116
Methylated C's in CHH context 879
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 380
Unmethylated C's in CHG context 662
Unmethylated C's in CHH context 3614
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 52.0%
Percentage methylation (CHG context) 14.9%
Percentage methylation (CHH context) 19.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 106 original top strand
CTOT 186 complementary to original top strand
CTOB 374 complementary to original bottom strand
OB 192 original bottom strand

Deduplication

Alignments analysed 858
Unique alignments 468
Duplicates removed 390
Duplicated alignments were found at 117 different positions

Cytosine Methylation after Extraction

Total C's analysed 3259
Methylated C's in CpG context 152
Methylated C's in CHG context 70
Methylated C's in CHH context 570
Unmethylated C's in CpG context 219
Unmethylated C's in CHG context 370
Unmethylated C's in CHH context 1878
Percentage methylation (CpG context) 41.0%
Percentage methylation (CHG context) 15.9%
Percentage methylation (CHH context) 23.3%

M-Bias Plot