Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_7_s456_trimmed.fq

Data processed at 21:26 on 2022-09-14


Alignment Stats

Sequences analysed in total 49730113
Single-end alignments with a unique best hit 45063
Sequences without alignments under any condition 49642436
Sequences that did not map uniquely 42614
Genomic sequence context not extractable (edges of chromosomes) 0
0.0906%99.8%0.0857%0%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 397458
Methylated C's in CpG context 9993
Methylated C's in CHG context 6260
Methylated C's in CHH context 82765
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 14084
Unmethylated C's in CHG context 27734
Unmethylated C's in CHH context 256622
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 41.5%
Percentage methylation (CHG context) 18.4%
Percentage methylation (CHH context) 24.4%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 11023 original top strand
CTOT 14490 complementary to original top strand
CTOB 11147 complementary to original bottom strand
OB 8403 original bottom strand
OTCTOTCTOBOB02k4k6k8k10k12k14k
Number of Alignments

Deduplication

Alignments analysed 45063
Unique alignments 21865
Duplicates removed 23198
Duplicated alignments were found at 3997 different positions
48.5%51.5%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 162283
Methylated C's in CpG context 6840
Methylated C's in CHG context 3096
Methylated C's in CHH context 34369
Unmethylated C's in CpG context 9616
Unmethylated C's in CHG context 14059
Unmethylated C's in CHH context 94303
Percentage methylation (CpG context) 41.6%
Percentage methylation (CHG context) 18.0%
Percentage methylation (CHH context) 26.7%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

51015202530020406080100010002000300040005000
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls