Alignment Stats
| Sequences analysed in total |
49730113 |
| Single-end alignments with a unique best hit |
45063 |
| Sequences without alignments under any condition |
49642436 |
| Sequences that did not map uniquely |
42614 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
397458 |
| Methylated C's in CpG context |
9993 |
| Methylated C's in CHG context |
6260 |
| Methylated C's in CHH context |
82765 |
| Methylated C's in Unknown context |
0 |
| Unmethylated C's in CpG context |
14084 |
| Unmethylated C's in CHG context |
27734 |
| Unmethylated C's in CHH context |
256622 |
| Unmethylated C's in Unknown context |
0 |
| Percentage methylation (CpG context) |
41.5% |
| Percentage methylation (CHG context) |
18.4% |
| Percentage methylation (CHH context) |
24.4% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
11023 |
original top strand |
| CTOT |
14490 |
complementary to original top strand |
| CTOB |
11147 |
complementary to original bottom strand |
| OB |
8403 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
45063 |
| Unique alignments |
21865 |
| Duplicates removed |
23198 |
| Duplicated alignments were found at 3997 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
162283 |
| Methylated C's in CpG context |
6840 |
| Methylated C's in CHG context |
3096 |
| Methylated C's in CHH context |
34369 |
| Unmethylated C's in CpG context |
9616 |
| Unmethylated C's in CHG context |
14059 |
| Unmethylated C's in CHH context |
94303 |
| Percentage methylation (CpG context) |
41.6% |
| Percentage methylation (CHG context) |
18.0% |
| Percentage methylation (CHH context) |
26.7% |
|
|
M-Bias Plot