Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq

Data processed at 21:26 on 2022-09-14


Alignment Stats

Sequences analysed in total 47401736
Single-end alignments with a unique best hit 49826
Sequences without alignments under any condition 47302825
Sequences that did not map uniquely 49085
Genomic sequence context not extractable (edges of chromosomes) 0
0.105%99.8%0.104%0%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 450928
Methylated C's in CpG context 8978
Methylated C's in CHG context 7241
Methylated C's in CHH context 111161
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 15009
Unmethylated C's in CHG context 28241
Unmethylated C's in CHH context 280298
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 37.4%
Percentage methylation (CHG context) 20.4%
Percentage methylation (CHH context) 28.4%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 11986 original top strand
CTOT 16666 complementary to original top strand
CTOB 12304 complementary to original bottom strand
OB 8870 original bottom strand
OTCTOTCTOBOB02k4k6k8k10k12k14k16k
Number of Alignments

Deduplication

Alignments analysed 49826
Unique alignments 22195
Duplicates removed 27631
Duplicated alignments were found at 4367 different positions
44.5%55.5%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 165255
Methylated C's in CpG context 5787
Methylated C's in CHG context 3061
Methylated C's in CHH context 38005
Unmethylated C's in CpG context 9997
Unmethylated C's in CHG context 13748
Unmethylated C's in CHH context 94657
Percentage methylation (CpG context) 36.7%
Percentage methylation (CHG context) 18.2%
Percentage methylation (CHH context) 28.6%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

510152025300204060801000100020003000400050006000
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls