Alignment Stats
| Sequences analysed in total |
42070546 |
| Single-end alignments with a unique best hit |
41080 |
| Sequences without alignments under any condition |
41994231 |
| Sequences that did not map uniquely |
35235 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
356764 |
| Methylated C's in CpG context |
10515 |
| Methylated C's in CHG context |
6264 |
| Methylated C's in CHH context |
87134 |
| Methylated C's in Unknown context |
0 |
| Unmethylated C's in CpG context |
12210 |
| Unmethylated C's in CHG context |
24224 |
| Unmethylated C's in CHH context |
216417 |
| Unmethylated C's in Unknown context |
0 |
| Percentage methylation (CpG context) |
46.3% |
| Percentage methylation (CHG context) |
20.5% |
| Percentage methylation (CHH context) |
28.7% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
10192 |
original top strand |
| CTOT |
12778 |
complementary to original top strand |
| CTOB |
10256 |
complementary to original bottom strand |
| OB |
7854 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
41080 |
| Unique alignments |
20003 |
| Duplicates removed |
21077 |
| Duplicated alignments were found at 3820 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
147288 |
| Methylated C's in CpG context |
6935 |
| Methylated C's in CHG context |
3064 |
| Methylated C's in CHH context |
34116 |
| Unmethylated C's in CpG context |
8570 |
| Unmethylated C's in CHG context |
12727 |
| Unmethylated C's in CHH context |
81876 |
| Percentage methylation (CpG context) |
44.7% |
| Percentage methylation (CHG context) |
19.4% |
| Percentage methylation (CHH context) |
29.4% |
|
|
M-Bias Plot