Alignment Stats
| Sequences analysed in total |
59459431 |
| Single-end alignments with a unique best hit |
56093 |
| Sequences without alignments under any condition |
59346303 |
| Sequences that did not map uniquely |
57035 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
504554 |
| Methylated C's in CpG context |
10126 |
| Methylated C's in CHG context |
6957 |
| Methylated C's in CHH context |
107706 |
| Methylated C's in Unknown context |
0 |
| Unmethylated C's in CpG context |
17416 |
| Unmethylated C's in CHG context |
33622 |
| Unmethylated C's in CHH context |
328727 |
| Unmethylated C's in Unknown context |
0 |
| Percentage methylation (CpG context) |
36.8% |
| Percentage methylation (CHG context) |
17.1% |
| Percentage methylation (CHH context) |
24.7% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
13926 |
original top strand |
| CTOT |
18047 |
complementary to original top strand |
| CTOB |
13674 |
complementary to original bottom strand |
| OB |
10446 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
56093 |
| Unique alignments |
24805 |
| Duplicates removed |
31288 |
| Duplicated alignments were found at 4831 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
184349 |
| Methylated C's in CpG context |
6654 |
| Methylated C's in CHG context |
3105 |
| Methylated C's in CHH context |
39493 |
| Unmethylated C's in CpG context |
11236 |
| Unmethylated C's in CHG context |
15761 |
| Unmethylated C's in CHH context |
108100 |
| Percentage methylation (CpG context) |
37.2% |
| Percentage methylation (CHG context) |
16.5% |
| Percentage methylation (CHH context) |
26.8% |
|
|
M-Bias Plot