Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-170_S13_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-170_S13_L002_R2_001_val_2.fq.gz

Data processed at 11:37 on 2019-08-07


Alignment Stats

Sequence pairs analysed in total 27996279
Paired-end alignments with a unique best hit 10233767
Pairs without alignments under any condition 15243065
Pairs that did not map uniquely 2519447
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 295230497
Methylated C's in CpG context 10743620
Methylated C's in CHG context 1476561
Methylated C's in CHH context 3272606
Methylated C's in Unknown context 54419
Unmethylated C's in CpG context 31907626
Unmethylated C's in CHG context 62815831
Unmethylated C's in CHH context 185014253
Unmethylated C's in Unknown context 638215
Percentage methylation (CpG context) 25.2%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 1.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5017772 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5215988 original bottom strand

Deduplication

Alignments analysed 10233760
Unique alignments 7797078
Duplicates removed 2436682
Duplicated alignments were found at 1417870 different positions

Cytosine Methylation after Extraction

Total C's analysed 151781122
Methylated C's in CpG context 5915753
Methylated C's in CHG context 746213
Methylated C's in CHH context 1797219
Unmethylated C's in CpG context 15089108
Unmethylated C's in CHG context 31172597
Unmethylated C's in CHH context 97060232
Percentage methylation (CpG context) 28.2%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 1.8%

M-Bias Plot