Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-199_S24_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-199_S24_L003_R2_001_val_2.fq.gz

Data processed at 08:15 on 2019-08-08


Alignment Stats

Sequence pairs analysed in total 15913069
Paired-end alignments with a unique best hit 6267321
Pairs without alignments under any condition 8113691
Pairs that did not map uniquely 1532057
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 181304638
Methylated C's in CpG context 8091308
Methylated C's in CHG context 993527
Methylated C's in CHH context 3522698
Methylated C's in Unknown context 63898
Unmethylated C's in CpG context 17646077
Unmethylated C's in CHG context 38210008
Unmethylated C's in CHH context 112841020
Unmethylated C's in Unknown context 386634
Percentage methylation (CpG context) 31.4%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 3.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3085988 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3181333 original bottom strand

Deduplication

Alignments analysed 6267321
Unique alignments 4367077
Duplicates removed 1900244
Duplicated alignments were found at 1204077 different positions

Cytosine Methylation after Extraction

Total C's analysed 88024093
Methylated C's in CpG context 4047323
Methylated C's in CHG context 479419
Methylated C's in CHH context 1871691
Unmethylated C's in CpG context 8070663
Unmethylated C's in CHG context 17975035
Unmethylated C's in CHH context 55579962
Percentage methylation (CpG context) 33.4%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 3.3%

M-Bias Plot