Bismark report for: /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-103_S27_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-103_S27_L005_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 20349659 Number of paired-end alignments with a unique best hit: 9233772 Mapping efficiency: 45.4% Sequence pairs with no alignments under any condition: 9350302 Sequence pairs did not map uniquely: 1765585 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4473147 ((converted) top strand) GA/CT/CT: 105161 (complementary to (converted) top strand) GA/CT/GA: 105157 (complementary to (converted) bottom strand) CT/GA/GA: 4550306 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 297460805 Total methylated C's in CpG context: 9677744 Total methylated C's in CHG context: 1380876 Total methylated C's in CHH context: 7299226 Total methylated C's in Unknown context: 88063 Total unmethylated C's in CpG context: 31148064 Total unmethylated C's in CHG context: 59966133 Total unmethylated C's in CHH context: 187988762 Total unmethylated C's in Unknown context: 637696 C methylated in CpG context: 23.7% C methylated in CHG context: 2.3% C methylated in CHH context: 3.7% C methylated in unknown context (CN or CHN): 12.1% Bismark completed in 0d 2h 6m 21s