Exception in thread "main" java.awt.HeadlessException: No X11 DISPLAY variable was set, but this program performed an operation which requires it. at java.awt.GraphicsEnvironment.checkHeadless(GraphicsEnvironment.java:204) at java.awt.Window.(Window.java:536) at java.awt.Frame.(Frame.java:420) at java.awt.Frame.(Frame.java:385) at javax.swing.JFrame.(JFrame.java:189) at uk.ac.babraham.FastQC.FastQCApplication.(FastQCApplication.java:63) at uk.ac.babraham.FastQC.FastQCApplication.main(FastQCApplication.java:338) *** Bismark methylation extractor version v0.22.3 *** Trying to determine the type of mapping from the SAM header line of file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Treating file(s) as paired-end data (as extracted from @PG line) Setting option '--no_overlap' since this is (normally) the right thing to do for paired-end data Core usage currently set to more than 20 threads. Let's see how this goes... (set value: 28) Summarising Bismark methylation extractor parameters: =============================================================== Bismark paired-end SAM format specified (default) Number of cores to be used: 28 Strand-specific outputs will be skipped. Separate output files for cytosines in CpG, CHG and CHH context will be generated Merge CHG and CHH context to non-CpG context specified Output will be written to the current directory ('/gscratch/scrubbed/sr320/071521-cvBS') Summarising bedGraph parameters: =============================================================== Generating additional output in bedGraph and coverage format bedGraph format: coverage format: Using a cutoff of 1 read(s) to report cytosine positions Reporting and sorting cytosine methylation information in CpG context only (default) The bedGraph UNIX sort command will use the following memory setting: '75%'. Temporary directory used for sorting is the output directory Checking file >>12M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >12M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/12M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/12M_R2_val_2.fq.gz" Now reading in Bismark result file 12M_R1_val_1_bismark_bt2_pe.deduplicated.bam Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 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Processed lines: 99500000 Processed lines: 97000000 Processed lines: 100000000 Processed lines: 96000000 Processed lines: 95500000 Processed lines: 95000000 Processed lines: 96500000 Processed lines: 94500000 Processed lines: 100500000 Processed lines: 97500000 Processed lines: 95000000 Processed lines: 98500000 Processed lines: 101000000 Processed lines: 95000000 Processed lines: 99500000 Processed lines: 96500000 Processed lines: 99000000 Processed lines: 98500000 Processed lines: 98000000 Processed lines: 97000000 Processed lines: 96000000 Processed lines: 101000000 Processed lines: 100500000 Now waiting for all child processes to complete Processed lines: 99000000 Processed lines: 95500000 Processed lines: 100500000 Processed lines: 97500000 Processed lines: 95000000 Processed lines: 100000000 Finished processing child process. Exiting.. Processed lines: 97500000 Processed lines: 100500000 Processed lines: 96500000 Processed lines: 96000000 Processed lines: 95500000 Processed lines: 97000000 Processed lines: 95000000 Processed lines: 101000000 Processed lines: 98000000 Processed lines: 99000000 Processed lines: 95500000 Processed lines: 100000000 Processed lines: 99500000 Processed lines: 95500000 Processed lines: 97000000 Processed lines: 99000000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 97500000 Processed lines: 96500000 Processed lines: 101000000 Processed lines: 99500000 Processed lines: 96000000 Processed lines: 101000000 Processed lines: 98000000 Processed lines: 100500000 Processed lines: 95500000 Finished processing child process. Exiting.. Processed lines: 101000000 Processed lines: 98000000 Finished processing child process. Exiting.. Processed lines: 96500000 Processed lines: 97000000 Processed lines: 97500000 Processed lines: 96000000 Processed lines: 95500000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 99500000 Processed lines: 96000000 Processed lines: 100500000 Processed lines: 100000000 Processed lines: 97500000 Processed lines: 99500000 Processed lines: 96000000 Processed lines: 99000000 Processed lines: 98000000 Processed lines: 100000000 Processed lines: 97000000 Processed lines: 96500000 Processed lines: 98500000 Processed lines: 101000000 Processed lines: 96000000 Processed lines: 98500000 Processed lines: 97000000 Finished processing child process. Exiting.. Processed lines: 97500000 Processed lines: 98000000 Processed lines: 96500000 Processed lines: 96000000 Processed lines: 99000000 Processed lines: 100000000 Processed lines: 96500000 Processed lines: 101000000 Processed lines: 100500000 Processed lines: 98000000 Processed lines: 100000000 Processed lines: 99500000 Processed lines: 96500000 Processed lines: 98500000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 97500000 Processed lines: 97000000 Processed lines: 99000000 Processed lines: 96500000 Processed lines: 99000000 Processed lines: 97500000 Processed lines: 98500000 Processed lines: 98000000 Processed lines: 97000000 Processed lines: 99500000 Processed lines: 100500000 Processed lines: 96500000 Processed lines: 97000000 Processed lines: 101000000 Processed lines: 98500000 Processed lines: 100500000 Processed lines: 100000000 Processed lines: 97000000 Processed lines: 99000000 Processed lines: 101000000 Finished processing child process. Exiting.. Processed lines: 98000000 Processed lines: 97500000 Processed lines: 99500000 Finished processing child process. Exiting.. Processed lines: 97000000 Processed lines: 99500000 Processed lines: 98000000 Processed lines: 98500000 Processed lines: 99000000 Processed lines: 97500000 Processed lines: 100000000 Processed lines: 101000000 Processed lines: 97000000 Processed lines: 97500000 Processed lines: 99000000 Processed lines: 101000000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 97500000 Processed lines: 99500000 Processed lines: 98500000 Finished processing child process. Exiting.. Processed lines: 98000000 Processed lines: 100000000 Processed lines: 97500000 Processed lines: 100000000 Processed lines: 98500000 Processed lines: 99000000 Processed lines: 99500000 Processed lines: 100500000 Processed lines: 98000000 Processed lines: 97500000 Processed lines: 98000000 Processed lines: 99500000 Processed lines: 101000000 Processed lines: 98000000 Processed lines: 100000000 Processed lines: 99000000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 98000000 Processed lines: 100500000 Processed lines: 99000000 Processed lines: 99500000 Processed lines: 100000000 Processed lines: 101000000 Processed lines: 98500000 Processed lines: 98000000 Processed lines: 98500000 Processed lines: 100000000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 100500000 Processed lines: 99500000 Processed lines: 101000000 Processed lines: 99000000 Processed lines: 98500000 Processed lines: 101000000 Finished processing child process. Exiting.. Processed lines: 99500000 Processed lines: 100000000 Processed lines: 100500000 Processed lines: 99000000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 99000000 Processed lines: 100500000 Processed lines: 99000000 Processed lines: 101000000 Processed lines: 100000000 Processed lines: 99500000 Finished processing child process. Exiting.. Processed lines: 99000000 Processed lines: 100500000 Processed lines: 101000000 Processed lines: 100000000 Processed lines: 99500000 Processed lines: 99000000 Processed lines: 101000000 Finished processing child process. Exiting.. Processed lines: 99500000 Processed lines: 99500000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 100000000 Processed lines: 99500000 Processed lines: 101000000 Processed lines: 100500000 Processed lines: 100000000 Finished processing child process. Exiting.. Processed lines: 99500000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 101000000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 100000000 Processed lines: 101000000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 100500000 Processed lines: 100000000 Processed lines: 100500000 Processed lines: 101000000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 101000000 Processed lines: 100500000 Processed lines: 101000000 Processed lines: 101000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 101000000 Finished processing child process. Exiting.. Processed lines: 101000000 Finished processing child process. Exiting.. Merging individual splitting reports into overall report: '12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 101143882 lines in total Total number of methylation call strings processed: 202287764 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2725400659 Total methylated C's in CpG context: 47153262 Total methylated C's in CHG context: 1834477 Total methylated C's in CHH context: 7382974 Total C to T conversions in CpG context: 302909324 Total C to T conversions in CHG context: 481680820 Total C to T conversions in CHH context: 1884439802 C methylated in CpG context: 13.5% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 12M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 12M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 12M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_12M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>13M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >13M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 13M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/13M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/13M_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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lines: 109500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 111500000 Processed lines: 109000000 Processed lines: 106500000 Processed lines: 104500000 Processed lines: 111500000 Processed lines: 107000000 Processed lines: 110500000 Processed lines: 112000000 Processed lines: 108000000 Processed lines: 111000000 Processed lines: 108500000 Processed lines: 110000000 Processed lines: 107500000 Processed lines: 105500000 Processed lines: 110500000 Processed lines: 109000000 Processed lines: 111500000 Processed lines: 106000000 Processed lines: 108000000 Processed lines: 112500000 Processed lines: 112500000 Processed lines: 106500000 Processed lines: 106000000 Processed lines: 106500000 Processed lines: 107500000 Processed lines: 110000000 Processed lines: 112000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 109500000 Processed lines: 107000000 Processed lines: 112000000 Processed lines: 105000000 Processed lines: 111000000 Processed lines: 112500000 Processed lines: 107500000 Processed lines: 111500000 Processed lines: 108500000 Finished processing child process. Exiting.. Processed lines: 109000000 Finished processing child process. Exiting.. Processed lines: 110500000 Processed lines: 108000000 Processed lines: 111000000 Processed lines: 106000000 Processed lines: 109500000 Processed lines: 112000000 Processed lines: 108500000 Processed lines: 106500000 Processed lines: 107000000 Processed lines: 110500000 Processed lines: 106500000 Processed lines: 108000000 Processed lines: 107000000 Processed lines: 112500000 Finished processing child process. Exiting.. Processed lines: 106500000 Processed lines: 110000000 Processed lines: 106500000 Processed lines: 107500000 Processed lines: 112500000 Processed lines: 105500000 Processed lines: 111500000 Processed lines: 112000000 Processed lines: 109000000 Processed lines: 108000000 Processed lines: 109500000 Processed lines: 111000000 Processed lines: 108500000 Processed lines: 110000000 Processed lines: 111500000 Finished processing child process. Exiting.. Processed lines: 106500000 Processed lines: 112500000 Processed lines: 109000000 Processed lines: 107000000 Processed lines: 107500000 Processed lines: 111000000 Finished processing child process. Exiting.. Processed lines: 108500000 Processed lines: 107500000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 110500000 Processed lines: 108000000 Processed lines: 107000000 Processed lines: 112000000 Finished processing child process. Exiting.. Processed lines: 112500000 Processed lines: 109500000 Processed lines: 106000000 Processed lines: 108500000 Processed lines: 110000000 Processed lines: 111500000 Processed lines: 109000000 Processed lines: 110500000 Processed lines: 112000000 Processed lines: 107000000 Processed lines: 109500000 Processed lines: 111500000 Processed lines: 107500000 Processed lines: 108000000 Processed lines: 109000000 Processed lines: 107500000 Processed lines: 108000000 Finished processing child process. Exiting.. Processed lines: 111000000 Processed lines: 107500000 Processed lines: 108500000 Processed lines: 107500000 Processed lines: 112500000 Processed lines: 110000000 Processed lines: 109000000 Processed lines: 106500000 Processed lines: 110500000 Processed lines: 112000000 Processed lines: 109500000 Processed lines: 111000000 Processed lines: 112500000 Processed lines: 110000000 Processed lines: 107500000 Processed lines: 112000000 Processed lines: 108000000 Processed lines: 108500000 Finished processing child process. Exiting.. Processed lines: 109500000 Processed lines: 108000000 Processed lines: 108500000 Processed lines: 111500000 Processed lines: 108000000 Processed lines: 109000000 Processed lines: 108000000 Finished processing child process. Exiting.. Processed lines: 110500000 Processed lines: 109500000 Processed lines: 107000000 Processed lines: 112500000 Processed lines: 111000000 Processed lines: 110000000 Processed lines: 111500000 Processed lines: 110500000 Processed lines: 108000000 Processed lines: 112500000 Processed lines: 108500000 Processed lines: 109000000 Processed lines: 110000000 Processed lines: 108500000 Processed lines: 109000000 Processed lines: 112000000 Finished processing child process. Exiting.. Processed lines: 108500000 Processed lines: 109500000 Processed lines: 108500000 Processed lines: 111000000 Processed lines: 110000000 Processed lines: 107500000 Processed lines: 111500000 Finished processing child process. Exiting.. Processed lines: 112000000 Processed lines: 110500000 Processed lines: 111000000 Processed lines: 108500000 Processed lines: 109500000 Processed lines: 110500000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109500000 Processed lines: 112500000 Processed lines: 110000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 111500000 Processed lines: 110500000 Processed lines: 112000000 Processed lines: 108000000 Processed lines: 112500000 Processed lines: 111000000 Processed lines: 111500000 Finished processing child process. Exiting.. Processed lines: 109000000 Processed lines: 111000000 Processed lines: 110000000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 110000000 Processed lines: 110500000 Processed lines: 109500000 Finished processing child process. Exiting.. Processed lines: 109500000 Processed lines: 112000000 Processed lines: 111000000 Processed lines: 112500000 Processed lines: 108500000 Processed lines: 111500000 Processed lines: 112000000 Processed lines: 109500000 Processed lines: 111500000 Processed lines: 110500000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110500000 Finished processing child process. Exiting.. Processed lines: 111000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 112500000 Processed lines: 111500000 Processed lines: 112000000 Processed lines: 109000000 Processed lines: 112500000 Processed lines: 110000000 Processed lines: 112000000 Processed lines: 111000000 Processed lines: 110500000 Finished processing child process. Exiting.. Processed lines: 110500000 Processed lines: 111000000 Processed lines: 111500000 Processed lines: 110500000 Processed lines: 110500000 Finished processing child process. Exiting.. Processed lines: 112000000 Processed lines: 112500000 Processed lines: 109500000 Processed lines: 110500000 Processed lines: 112500000 Processed lines: 111500000 Processed lines: 111000000 Processed lines: 111000000 Processed lines: 111500000 Processed lines: 112000000 Processed lines: 111000000 Processed lines: 111000000 Finished processing child process. Exiting.. Processed lines: 112500000 Finished processing child process. Exiting.. Processed lines: 110000000 Processed lines: 111000000 Processed lines: 111500000 Processed lines: 112000000 Processed lines: 112000000 Processed lines: 111500000 Finished processing child process. Exiting.. Processed lines: 112500000 Processed lines: 111500000 Processed lines: 111500000 Processed lines: 110500000 Processed lines: 111500000 Finished processing child process. Exiting.. Processed lines: 112000000 Processed lines: 112500000 Processed lines: 112500000 Processed lines: 112000000 Processed lines: 112000000 Processed lines: 112000000 Processed lines: 111000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 112000000 Processed lines: 112500000 Processed lines: 112500000 Processed lines: 112500000 Processed lines: 112500000 Finished processing child process. Exiting.. Processed lines: 111500000 Processed lines: 112500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 112000000 Processed lines: 112500000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 112840017 lines in total Total number of methylation call strings processed: 225680034 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2895601370 Total methylated C's in CpG context: 49622161 Total methylated C's in CHG context: 2041725 Total methylated C's in CHH context: 8365379 Total C to T conversions in CpG context: 336438857 Total C to T conversions in CHG context: 523821535 Total C to T conversions in CHH context: 1975311713 C methylated in CpG context: 12.9% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 13M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 13M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 13M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_13M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>16F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >16F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 16F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/16F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/16F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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Processed lines: 88500000 Processed lines: 93000000 Processed lines: 92000000 Processed lines: 88000000 Processed lines: 86500000 Processed lines: 91500000 Processed lines: 90500000 Processed lines: 89000000 Processed lines: 87500000 Processed lines: 86500000 Processed lines: 88500000 Processed lines: 89500000 Processed lines: 86500000 Processed lines: 90000000 Processed lines: 91000000 Processed lines: 87000000 Processed lines: 91500000 Processed lines: 86000000 Processed lines: 92500000 Processed lines: 88000000 Processed lines: 91500000 Processed lines: 90500000 Processed lines: 87500000 Processed lines: 87000000 Processed lines: 93000000 Processed lines: 86000000 Processed lines: 90500000 Processed lines: 86500000 Processed lines: 89000000 Processed lines: 93500000 Processed lines: 92500000 Processed lines: 92000000 Processed lines: 88500000 Processed lines: 91000000 Processed lines: 87000000 Processed lines: 89500000 Processed lines: 88000000 Processed lines: 87000000 Processed lines: 89000000 Processed lines: 90000000 Processed lines: 90500000 Processed lines: 87000000 Processed lines: 91500000 Processed lines: 92000000 Processed lines: 87500000 Processed lines: 93000000 Processed lines: 86500000 Processed lines: 88500000 Processed lines: 91000000 Processed lines: 92000000 Processed lines: 88000000 Processed lines: 93500000 Processed lines: 87500000 Processed lines: 94000000 Processed lines: 91000000 Processed lines: 86500000 Processed lines: 87000000 Processed lines: 89500000 Processed lines: 93000000 Processed lines: 92500000 Processed lines: 91500000 Processed lines: 89000000 Processed lines: 87500000 Processed lines: 90000000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 87500000 Processed lines: 90500000 Processed lines: 89500000 Processed lines: 91000000 Processed lines: 92000000 Processed lines: 87500000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 88000000 Processed lines: 87000000 Processed lines: 92500000 Processed lines: 91500000 Processed lines: 89000000 Processed lines: 94000000 Processed lines: 88500000 Processed lines: 88000000 Processed lines: 91500000 Processed lines: 93500000 Processed lines: 90000000 Processed lines: 87500000 Processed lines: 87000000 Processed lines: 93000000 Processed lines: 92000000 Processed lines: 89500000 Finished processing child process. Exiting.. Processed lines: 88000000 Processed lines: 90500000 Processed lines: 89000000 Processed lines: 91000000 Processed lines: 91500000 Processed lines: 90000000 Processed lines: 92500000 Processed lines: 88000000 Processed lines: 93000000 Processed lines: 94000000 Processed lines: 88000000 Processed lines: 88500000 Processed lines: 92000000 Processed lines: 93000000 Processed lines: 87500000 Processed lines: 89500000 Processed lines: 89000000 Finished processing child process. Exiting.. Processed lines: 88500000 Processed lines: 94000000 Processed lines: 92000000 Processed lines: 90500000 Processed lines: 93500000 Processed lines: 88000000 Processed lines: 87500000 Processed lines: 92500000 Processed lines: 90000000 Processed lines: 91000000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 92000000 Processed lines: 91500000 Processed lines: 93000000 Processed lines: 90500000 Processed lines: 93500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 89000000 Processed lines: 90000000 Processed lines: 88000000 Processed lines: 89500000 Processed lines: 92500000 Processed lines: 89000000 Processed lines: 94000000 Processed lines: 91000000 Processed lines: 93000000 Processed lines: 88500000 Processed lines: 88000000 Processed lines: 90500000 Processed lines: 91500000 Processed lines: 89000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 93500000 Processed lines: 92000000 Processed lines: 94000000 Processed lines: 91000000 Processed lines: 89000000 Processed lines: 93000000 Processed lines: 94000000 Processed lines: 89000000 Processed lines: 89500000 Processed lines: 90500000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 90000000 Processed lines: 93000000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 91500000 Processed lines: 93500000 Processed lines: 89000000 Processed lines: 91000000 Processed lines: 88500000 Processed lines: 92000000 Processed lines: 89500000 Processed lines: 90500000 Processed lines: 93000000 Processed lines: 94000000 Processed lines: 92500000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 93500000 Processed lines: 89500000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 91000000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 93500000 Processed lines: 92000000 Processed lines: 90000000 Processed lines: 94000000 Processed lines: 91500000 Processed lines: 92500000 Processed lines: 89000000 Processed lines: 89500000 Processed lines: 90000000 Processed lines: 93500000 Finished processing child process. Exiting.. Processed lines: 91000000 Processed lines: 93000000 Processed lines: 92000000 Processed lines: 94000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90500000 Processed lines: 91500000 Processed lines: 94000000 Processed lines: 89500000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 90500000 Processed lines: 92000000 Processed lines: 93000000 Processed lines: 90000000 Processed lines: 89500000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 94000000 Processed lines: 91500000 Processed lines: 93500000 Processed lines: 92500000 Processed lines: 90500000 Processed lines: 90500000 Finished processing child process. Exiting.. Processed lines: 92000000 Processed lines: 91000000 Processed lines: 91500000 Processed lines: 90000000 Processed lines: 93000000 Processed lines: 91000000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 90500000 Processed lines: 90000000 Processed lines: 91000000 Processed lines: 92000000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 91000000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 91500000 Processed lines: 92000000 Processed lines: 90500000 Processed lines: 93500000 Processed lines: 91500000 Processed lines: 93000000 Processed lines: 94000000 Processed lines: 91000000 Processed lines: 90500000 Processed lines: 91500000 Processed lines: 92500000 Processed lines: 93500000 Finished processing child process. Exiting.. Processed lines: 91500000 Processed lines: 91500000 Processed lines: 93000000 Processed lines: 92000000 Processed lines: 92500000 Processed lines: 94000000 Processed lines: 91000000 Processed lines: 92000000 Processed lines: 93500000 Processed lines: 91500000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 92000000 Processed lines: 93000000 Processed lines: 94000000 Processed lines: 92000000 Processed lines: 92000000 Processed lines: 93500000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 93000000 Processed lines: 91500000 Processed lines: 92500000 Processed lines: 94000000 Processed lines: 92000000 Processed lines: 93500000 Processed lines: 91500000 Processed lines: 92500000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 92500000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 93500000 Processed lines: 92000000 Processed lines: 93000000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 92000000 Processed lines: 93000000 Processed lines: 93000000 Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 94000000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 93000000 Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 93500000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 94000000 Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 94000000 Processed lines: 93000000 Finished processing child process. Exiting.. Processed lines: 94000000 Finished processing child process. Exiting.. Processed lines: 94000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 94000000 Processed lines: 93500000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 94000000 Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 94179724 lines in total Total number of methylation call strings processed: 188359448 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2377770409 Total methylated C's in CpG context: 37346078 Total methylated C's in CHG context: 1784502 Total methylated C's in CHH context: 7425685 Total C to T conversions in CpG context: 268205283 Total C to T conversions in CHG context: 418913612 Total C to T conversions in CHH context: 1644095249 C methylated in CpG context: 12.2% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 16F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 16F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 16F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_16F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>19F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >19F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 19F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/19F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/19F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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Processed lines: 96500000 Processed lines: 94000000 Processed lines: 91500000 Processed lines: 91500000 Processed lines: 91500000 Processed lines: 95500000 Processed lines: 97000000 Processed lines: 95000000 Processed lines: 94000000 Processed lines: 96500000 Processed lines: 92000000 Processed lines: 92000000 Processed lines: 96000000 Processed lines: 93500000 Processed lines: 96500000 Processed lines: 95500000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 96500000 Processed lines: 98000000 Processed lines: 94500000 Processed lines: 94000000 Processed lines: 96500000 Processed lines: 92500000 Processed lines: 92500000 Processed lines: 93000000 Processed lines: 93500000 Processed lines: 95000000 Processed lines: 96000000 Processed lines: 97000000 Processed lines: 97500000 Processed lines: 94500000 Processed lines: 92000000 Processed lines: 92000000 Processed lines: 92000000 Processed lines: 95500000 Processed lines: 97000000 Processed lines: 94500000 Processed lines: 96500000 Processed lines: 97000000 Processed lines: 94000000 Processed lines: 92500000 Processed lines: 92500000 Processed lines: 96000000 Processed lines: 97500000 Processed lines: 97500000 Processed lines: 97000000 Processed lines: 98500000 Processed lines: 97000000 Processed lines: 95000000 Processed lines: 94500000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 95500000 Processed lines: 96500000 Processed lines: 97500000 Processed lines: 94000000 Processed lines: 93500000 Processed lines: 98000000 Processed lines: 95000000 Processed lines: 96000000 Processed lines: 92500000 Processed lines: 92500000 Processed lines: 92500000 Processed lines: 97500000 Processed lines: 95000000 Processed lines: 97500000 Processed lines: 97000000 Processed lines: 94500000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 96500000 Processed lines: 97500000 Processed lines: 99000000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 97500000 Processed lines: 95000000 Processed lines: 95500000 Processed lines: 93500000 Processed lines: 98000000 Processed lines: 93500000 Processed lines: 96000000 Processed lines: 97000000 Processed lines: 98500000 Processed lines: 94500000 Processed lines: 95500000 Processed lines: 94000000 Processed lines: 96500000 Processed lines: 98000000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 98000000 Processed lines: 97500000 Processed lines: 95500000 Processed lines: 95000000 Processed lines: 97000000 Processed lines: 93500000 Processed lines: 93500000 Processed lines: 99500000 Processed lines: 98000000 Processed lines: 98500000 Processed lines: 98500000 Finished processing child process. Exiting.. Processed lines: 98000000 Processed lines: 95500000 Processed lines: 98500000 Processed lines: 96000000 Processed lines: 99000000 Processed lines: 96500000 Processed lines: 97500000 Processed lines: 94000000 Processed lines: 94000000 Processed lines: 95000000 Processed lines: 96000000 Processed lines: 97000000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 94500000 Processed lines: 93500000 Processed lines: 93500000 Processed lines: 93500000 Processed lines: 98000000 Processed lines: 96000000 Processed lines: 95500000 Processed lines: 97500000 Processed lines: 98500000 Processed lines: 94000000 Processed lines: 94000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 98500000 Processed lines: 99500000 Processed lines: 99000000 Processed lines: 96500000 Processed lines: 96000000 Processed lines: 97000000 Processed lines: 98000000 Processed lines: 94500000 Finished processing child process. Exiting.. Processed lines: 95500000 Processed lines: 99000000 Processed lines: 94500000 Processed lines: 97500000 Processed lines: 96500000 Processed lines: 99000000 Processed lines: 98500000 Processed lines: 94000000 Processed lines: 94000000 Processed lines: 94000000 Processed lines: 95000000 Processed lines: 96500000 Processed lines: 98000000 Processed lines: 96000000 Processed lines: 99000000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 94500000 Processed lines: 94500000 Processed lines: 99000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 99500000 Processed lines: 97000000 Processed lines: 96500000 Processed lines: 97500000 Processed lines: 98500000 Finished processing child process. Exiting.. Processed lines: 99500000 Processed lines: 96000000 Processed lines: 95000000 Processed lines: 98000000 Processed lines: 95000000 Processed lines: 97000000 Finished processing child process. Exiting.. Processed lines: 99500000 Processed lines: 99000000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 94500000 Processed lines: 94500000 Processed lines: 94500000 Processed lines: 96500000 Processed lines: 95500000 Processed lines: 97000000 Processed lines: 99500000 Finished processing child process. Exiting.. Processed lines: 99500000 Processed lines: 95000000 Processed lines: 95000000 Finished processing child process. Exiting.. Processed lines: 97500000 Processed lines: 97000000 Processed lines: 98000000 Processed lines: 99000000 Processed lines: 96500000 Processed lines: 98500000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 97500000 Processed lines: 99500000 Processed lines: 99000000 Finished processing child process. Exiting.. Processed lines: 97000000 Processed lines: 97500000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 96000000 Processed lines: 98000000 Processed lines: 97500000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 98500000 Processed lines: 99500000 Processed lines: 99000000 Processed lines: 97000000 Finished processing child process. Exiting.. Processed lines: 96000000 Processed lines: 96000000 Processed lines: 98000000 Processed lines: 99500000 Processed lines: 97500000 Processed lines: 98000000 Finished processing child process. Exiting.. Processed lines: 95500000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 96500000 Processed lines: 98500000 Processed lines: 98000000 Processed lines: 99000000 Processed lines: 96000000 Processed lines: 96000000 Processed lines: 99500000 Processed lines: 97500000 Finished processing child process. Exiting.. Processed lines: 96500000 Processed lines: 96500000 Processed lines: 98000000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 96000000 Processed lines: 96000000 Processed lines: 96000000 Processed lines: 99000000 Processed lines: 97000000 Processed lines: 98500000 Processed lines: 99500000 Finished processing child process. Exiting.. Processed lines: 98000000 Processed lines: 96500000 Processed lines: 96500000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 98500000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 96500000 Processed lines: 96500000 Processed lines: 96500000 Processed lines: 99500000 Processed lines: 99000000 Processed lines: 97500000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 97500000 Processed lines: 97500000 Processed lines: 99000000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 97000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 99500000 Finished processing child process. Exiting.. Processed lines: 98000000 Processed lines: 99000000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 99500000 Processed lines: 97500000 Processed lines: 97500000 Finished processing child process. Exiting.. Processed lines: 97500000 Processed lines: 97500000 Processed lines: 97500000 Processed lines: 99500000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 99571767 lines in total Total number of methylation call strings processed: 199143534 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2486290912 Total methylated C's in CpG context: 38715437 Total methylated C's in CHG context: 1881993 Total methylated C's in CHH context: 8347696 Total C to T conversions in CpG context: 284892928 Total C to T conversions in CHG context: 443141610 Total C to T conversions in CHH context: 1709311248 C methylated in CpG context: 12.0% C methylated in non-CpG context: 0.5% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 19F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 19F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 19F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_19F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Finished BedGraph conversion ... Checking file >>22F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >22F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 22F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/22F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/22F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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60500000 Processed lines: 60500000 Processed lines: 60500000 Processed lines: 60500000 Processed lines: 62500000 Processed lines: 64000000 Processed lines: 64500000 Processed lines: 65000000 Processed lines: 66000000 Processed lines: 62000000 Processed lines: 65000000 Processed lines: 61000000 Processed lines: 65500000 Processed lines: 64000000 Processed lines: 63500000 Processed lines: 63000000 Processed lines: 64500000 Processed lines: 61500000 Processed lines: 61500000 Processed lines: 64000000 Processed lines: 65500000 Processed lines: 66000000 Processed lines: 65000000 Processed lines: 63000000 Processed lines: 63500000 Processed lines: 60000000 Processed lines: 65500000 Processed lines: 64500000 Processed lines: 63000000 Processed lines: 65000000 Processed lines: 66500000 Processed lines: 61000000 Processed lines: 61000000 Processed lines: 61000000 Processed lines: 61000000 Processed lines: 61000000 Processed lines: 61000000 Processed lines: 65500000 Processed lines: 62500000 Processed lines: 66000000 Processed lines: 61500000 Processed lines: 64500000 Finished processing child process. Exiting.. Processed lines: 64000000 Processed lines: 63500000 Processed lines: 65000000 Processed lines: 64500000 Processed lines: 66000000 Processed lines: 66500000 Processed lines: 62000000 Processed lines: 62000000 Processed lines: 65500000 Processed lines: 63500000 Processed lines: 64000000 Processed lines: 66000000 Processed lines: 65000000 Processed lines: 65500000 Processed lines: 63500000 Processed lines: 66000000 Processed lines: 60500000 Processed lines: 61500000 Processed lines: 61500000 Processed lines: 61500000 Processed lines: 61500000 Processed lines: 61500000 Processed lines: 61500000 Finished processing child process. Exiting.. Processed lines: 63000000 Processed lines: 66500000 Processed lines: 65000000 Processed lines: 62000000 Processed lines: 64500000 Processed lines: 65500000 Processed lines: 64000000 Processed lines: 65000000 Processed lines: 66500000 Processed lines: 66000000 Finished processing child process. Exiting.. Processed lines: 64500000 Processed lines: 62500000 Processed lines: 62500000 Processed lines: 64000000 Processed lines: 66500000 Processed lines: 65500000 Processed lines: 66000000 Processed lines: 66500000 Processed lines: 64000000 Finished processing child process. Exiting.. Processed lines: 61000000 Processed lines: 62000000 Processed lines: 62000000 Processed lines: 62000000 Processed lines: 62000000 Processed lines: 62000000 Processed lines: 62000000 Processed lines: 63500000 Processed lines: 65500000 Finished processing child process. Exiting.. Processed lines: 62500000 Processed lines: 65000000 Finished processing child process. Exiting.. Processed lines: 66000000 Processed lines: 64500000 Processed lines: 65500000 Processed lines: 66500000 Processed lines: 65000000 Processed lines: 64500000 Processed lines: 63000000 Processed lines: 63000000 Processed lines: 66000000 Processed lines: 66500000 Processed lines: 64500000 Finished processing child process. Exiting.. Processed lines: 61500000 Processed lines: 62500000 Processed lines: 62500000 Processed lines: 62500000 Processed lines: 64000000 Processed lines: 62500000 Processed lines: 62500000 Processed lines: 62500000 Processed lines: 66000000 Finished processing child process. Exiting.. Processed lines: 65500000 Processed lines: 63000000 Processed lines: 66500000 Processed lines: 66000000 Processed lines: 65000000 Processed lines: 65500000 Processed lines: 65000000 Processed lines: 66500000 Finished processing child process. Exiting.. Processed lines: 63500000 Processed lines: 63500000 Processed lines: 65000000 Processed lines: 62000000 Processed lines: 66500000 Processed lines: 64500000 Finished processing child process. Exiting.. Processed lines: 63000000 Processed lines: 63000000 Processed lines: 63000000 Processed lines: 63000000 Processed lines: 63000000 Processed lines: 63000000 Processed lines: 66000000 Processed lines: 63500000 Processed lines: 66500000 Processed lines: 65500000 Processed lines: 66000000 Finished processing child process. Exiting.. Processed lines: 65500000 Finished processing child process. Exiting.. Processed lines: 65500000 Processed lines: 64000000 Processed lines: 64000000 Processed lines: 62500000 Processed lines: 65000000 Processed lines: 66500000 Processed lines: 63500000 Processed lines: 63500000 Processed lines: 63500000 Processed lines: 63500000 Processed lines: 63500000 Processed lines: 63500000 Processed lines: 64000000 Processed lines: 66000000 Processed lines: 66500000 Processed lines: 66000000 Finished processing child process. Exiting.. Processed lines: 66000000 Processed lines: 64500000 Processed lines: 64500000 Finished processing child process. Exiting.. Processed lines: 65500000 Processed lines: 63000000 Processed lines: 64500000 Processed lines: 66500000 Processed lines: 64000000 Processed lines: 64000000 Processed lines: 64000000 Processed lines: 64000000 Processed lines: 64000000 Processed lines: 64000000 Processed lines: 66500000 Processed lines: 66500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 65000000 Processed lines: 65000000 Processed lines: 66000000 Processed lines: 65000000 Finished processing child process. Exiting.. Processed lines: 63500000 Processed lines: 64500000 Processed lines: 64500000 Processed lines: 64500000 Processed lines: 64500000 Processed lines: 64500000 Processed lines: 64500000 Processed lines: 65500000 Processed lines: 65500000 Processed lines: 65500000 Processed lines: 66500000 Processed lines: 64000000 Processed lines: 65000000 Processed lines: 65000000 Processed lines: 65000000 Processed lines: 65000000 Processed lines: 65000000 Processed lines: 65000000 Finished processing child process. Exiting.. Processed lines: 65500000 Processed lines: 65500000 Processed lines: 65500000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 65500000 Processed lines: 65500000 Processed lines: 65500000 Processed lines: 64500000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 65000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 66500000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 65500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 66000000 Processed lines: 66500000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 66746852 lines in total Total number of methylation call strings processed: 133493704 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1932255721 Total methylated C's in CpG context: 28515624 Total methylated C's in CHG context: 1366744 Total methylated C's in CHH context: 5635575 Total C to T conversions in CpG context: 210931753 Total C to T conversions in CHG context: 334520859 Total C to T conversions in CHH context: 1351285166 C methylated in CpG context: 11.9% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 22F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 22F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 22F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_22F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Finished BedGraph conversion ... Checking file >>23M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >23M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 23M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/23M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/23M_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 110500000 Processed lines: 101500000 Processed lines: 104500000 Processed lines: 107500000 Finished processing child process. Exiting.. Processed lines: 106500000 Processed lines: 106000000 Processed lines: 105000000 Processed lines: 107000000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 100000000 Processed lines: 105500000 Processed lines: 106000000 Processed lines: 102500000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 107000000 Processed lines: 103500000 Processed lines: 106500000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 102000000 Processed lines: 105000000 Processed lines: 108000000 Processed lines: 107000000 Processed lines: 106500000 Processed lines: 105500000 Processed lines: 107500000 Processed lines: 106000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 100500000 Processed lines: 106500000 Processed lines: 107500000 Processed lines: 103000000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 104000000 Processed lines: 107000000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 102500000 Processed lines: 105500000 Processed lines: 108500000 Processed lines: 107500000 Processed lines: 107000000 Processed lines: 106000000 Processed lines: 108000000 Processed lines: 106500000 Processed lines: 107000000 Processed lines: 102500000 Processed lines: 101000000 Processed lines: 108000000 Processed lines: 102500000 Processed lines: 101000000 Processed lines: 107500000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 103500000 Processed lines: 104500000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 106000000 Processed lines: 103000000 Processed lines: 109000000 Processed lines: 108000000 Processed lines: 107500000 Processed lines: 106500000 Processed lines: 108500000 Processed lines: 107000000 Processed lines: 107500000 Processed lines: 108500000 Processed lines: 101500000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 108000000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 105000000 Processed lines: 104000000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 106500000 Processed lines: 109500000 Processed lines: 108500000 Processed lines: 103500000 Processed lines: 108000000 Processed lines: 107000000 Processed lines: 109000000 Processed lines: 107500000 Processed lines: 109000000 Processed lines: 108000000 Processed lines: 102000000 Processed lines: 108500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 105500000 Processed lines: 107000000 Processed lines: 104500000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 110000000 Processed lines: 109000000 Processed lines: 104000000 Processed lines: 108500000 Processed lines: 107500000 Processed lines: 109500000 Processed lines: 108000000 Processed lines: 109500000 Processed lines: 108500000 Processed lines: 109000000 Processed lines: 102500000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 107500000 Processed lines: 110500000 Processed lines: 106000000 Processed lines: 109500000 Processed lines: 101500000 Processed lines: 105000000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 109000000 Processed lines: 104500000 Processed lines: 108000000 Processed lines: 110000000 Processed lines: 108500000 Finished processing child process. Exiting.. Processed lines: 110000000 Processed lines: 109500000 Processed lines: 103000000 Processed lines: 109000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 108000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 110000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 109500000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 105500000 Processed lines: 106500000 Processed lines: 108500000 Processed lines: 110500000 Processed lines: 109000000 Processed lines: 105000000 Processed lines: 110500000 Finished processing child process. Exiting.. Processed lines: 110000000 Finished processing child process. Exiting.. Processed lines: 103500000 Processed lines: 109500000 Processed lines: 108500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 110500000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 110000000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 109000000 Processed lines: 107000000 Processed lines: 106000000 Processed lines: 109500000 Finished processing child process. Exiting.. Processed lines: 105500000 Processed lines: 110500000 Processed lines: 104000000 Processed lines: 110000000 Finished processing child process. Exiting.. Processed lines: 109000000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 110500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 109500000 Processed lines: 110000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 107500000 Processed lines: 106500000 Finished processing child process. Exiting.. Processed lines: 106000000 Processed lines: 104500000 Processed lines: 110500000 Processed lines: 109500000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 110500000 Finished processing child process. Exiting.. Processed lines: 110000000 Processed lines: 108000000 Processed lines: 107000000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Finished processing child process. Exiting.. Processed lines: 106500000 Processed lines: 110000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 110500000 Processed lines: 108500000 Finished processing child process. Exiting.. Processed lines: 107500000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 104000000 Processed lines: 107000000 Processed lines: 110500000 Processed lines: 107000000 Processed lines: 107000000 Finished processing child process. Exiting.. Processed lines: 105500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 109000000 Processed lines: 108000000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 104500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 109500000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 105000000 Processed lines: 108500000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 110000000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 105500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 109000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 110500000 Finished processing child process. Exiting.. Processed lines: 109000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 106000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 106500000 Processed lines: 110000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 107000000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 110500000 Processed lines: 110500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Processed lines: 107500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 108000000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 108500000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 109000000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 110500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 109500000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110000000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 110500000 Processed lines: 110500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 110649723 lines in total Total number of methylation call strings processed: 221299446 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2894655837 Total methylated C's in CpG context: 51270609 Total methylated C's in CHG context: 2002961 Total methylated C's in CHH context: 8414346 Total C to T conversions in CpG context: 325736336 Total C to T conversions in CHG context: 516029177 Total C to T conversions in CHH context: 1991202408 C methylated in CpG context: 13.6% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 23M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 23M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 23M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_23M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>29F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >29F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/29F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/29F_R2_val_2.fq.gz" Now reading in Bismark result file 29F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. 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65500000 Processed lines: 67500000 Processed lines: 67000000 Processed lines: 69500000 Processed lines: 65500000 Processed lines: 65500000 Processed lines: 65500000 Processed lines: 65500000 Processed lines: 70500000 Processed lines: 68500000 Processed lines: 64500000 Processed lines: 66500000 Processed lines: 69000000 Processed lines: 67500000 Processed lines: 70000000 Processed lines: 67500000 Processed lines: 68500000 Processed lines: 68000000 Processed lines: 70000000 Processed lines: 68000000 Processed lines: 68500000 Processed lines: 72000000 Processed lines: 70500000 Processed lines: 67000000 Processed lines: 69000000 Processed lines: 71000000 Processed lines: 67000000 Processed lines: 70000000 Processed lines: 67500000 Processed lines: 68500000 Processed lines: 71000000 Processed lines: 66000000 Processed lines: 68000000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 66000000 Processed lines: 69000000 Processed lines: 66000000 Processed lines: 69500000 Processed lines: 65000000 Processed lines: 67000000 Processed lines: 70500000 Processed lines: 68000000 Processed lines: 68000000 Processed lines: 69000000 Processed lines: 70500000 Processed lines: 68500000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 69000000 Processed lines: 68500000 Processed lines: 67500000 Processed lines: 71500000 Processed lines: 69500000 Processed lines: 70500000 Processed lines: 67500000 Processed lines: 71500000 Processed lines: 68000000 Processed lines: 69500000 Processed lines: 69000000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 68500000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 66500000 Processed lines: 70000000 Processed lines: 67500000 Processed lines: 71000000 Finished processing child process. Exiting.. Processed lines: 68500000 Processed lines: 65500000 Processed lines: 71000000 Processed lines: 68500000 Processed lines: 69500000 Processed lines: 69000000 Processed lines: 71500000 Processed lines: 69000000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 68000000 Processed lines: 71000000 Processed lines: 70000000 Processed lines: 72000000 Processed lines: 68500000 Processed lines: 70000000 Processed lines: 68000000 Processed lines: 69500000 Processed lines: 70500000 Processed lines: 67000000 Processed lines: 67000000 Processed lines: 67000000 Processed lines: 67000000 Processed lines: 67000000 Processed lines: 69000000 Processed lines: 71500000 Processed lines: 68000000 Processed lines: 69000000 Processed lines: 71500000 Processed lines: 70000000 Processed lines: 69500000 Processed lines: 66000000 Processed lines: 69000000 Processed lines: 72000000 Processed lines: 72500000 Processed lines: 69500000 Processed lines: 68500000 Processed lines: 70000000 Processed lines: 71500000 Processed lines: 70500000 Processed lines: 72500000 Processed lines: 70500000 Processed lines: 69000000 Processed lines: 68500000 Processed lines: 71000000 Processed lines: 70000000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 67500000 Processed lines: 67500000 Processed lines: 67500000 Processed lines: 67500000 Processed lines: 67500000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 68500000 Processed lines: 70500000 Finished processing child process. Exiting.. Processed lines: 70000000 Processed lines: 72500000 Processed lines: 69500000 Processed lines: 66500000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 69000000 Processed lines: 70000000 Processed lines: 71000000 Processed lines: 70500000 Processed lines: 69500000 Processed lines: 71000000 Processed lines: 71500000 Processed lines: 70000000 Processed lines: 72500000 Processed lines: 70500000 Processed lines: 69000000 Finished processing child process. Exiting.. Processed lines: 68000000 Processed lines: 68000000 Processed lines: 68000000 Processed lines: 68000000 Processed lines: 68000000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 70000000 Processed lines: 70500000 Processed lines: 69000000 Processed lines: 70000000 Processed lines: 67000000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 69500000 Processed lines: 70500000 Finished processing child process. Exiting.. Processed lines: 71000000 Processed lines: 70000000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 70500000 Processed lines: 71500000 Processed lines: 71000000 Processed lines: 69500000 Processed lines: 71500000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 71000000 Processed lines: 70500000 Finished processing child process. Exiting.. Processed lines: 69500000 Processed lines: 70500000 Processed lines: 67500000 Processed lines: 72000000 Processed lines: 70000000 Processed lines: 71000000 Processed lines: 70500000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 72000000 Processed lines: 71000000 Processed lines: 71500000 Processed lines: 72000000 Processed lines: 70000000 Processed lines: 71500000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 71000000 Processed lines: 70000000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 70500000 Processed lines: 68000000 Finished processing child process. Exiting.. Processed lines: 71500000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72500000 Finished processing child process. Exiting.. Processed lines: 71500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 71500000 Processed lines: 71000000 Processed lines: 68500000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 71500000 Processed lines: 72500000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 71500000 Processed lines: 71000000 Processed lines: 71000000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 72000000 Processed lines: 69000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 72500000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 72000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 69500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 72500000 Finished processing child process. Exiting.. Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 70000000 Finished processing child process. Exiting.. Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 70500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 71000000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 71500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 72500000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 72910041 lines in total Total number of methylation call strings processed: 145820082 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2061199527 Total methylated C's in CpG context: 29672752 Total methylated C's in CHG context: 1416706 Total methylated C's in CHH context: 5937298 Total C to T conversions in CpG context: 228068733 Total C to T conversions in CHG context: 355765857 Total C to T conversions in CHH context: 1440338181 C methylated in CpG context: 11.5% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 29F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 29F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 29F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_29F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Finished BedGraph conversion ... Checking file >>31M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >31M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 31M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/31M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/31M_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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lines: 73500000 Processed lines: 72500000 Processed lines: 75000000 Processed lines: 72000000 Processed lines: 74500000 Processed lines: 71500000 Processed lines: 73500000 Processed lines: 74000000 Processed lines: 72000000 Processed lines: 72500000 Processed lines: 75500000 Processed lines: 76000000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 74500000 Processed lines: 72000000 Processed lines: 76500000 Processed lines: 71000000 Processed lines: 74000000 Processed lines: 71000000 Processed lines: 76000000 Processed lines: 71000000 Processed lines: 73000000 Processed lines: 73500000 Processed lines: 72000000 Processed lines: 74000000 Processed lines: 75500000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 75000000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 72000000 Processed lines: 76000000 Processed lines: 76500000 Processed lines: 72500000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 73000000 Processed lines: 75500000 Processed lines: 75000000 Processed lines: 73000000 Processed lines: 72000000 Processed lines: 77000000 Processed lines: 76500000 Processed lines: 72500000 Processed lines: 74500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 73500000 Processed lines: 71500000 Processed lines: 74000000 Processed lines: 76000000 Processed lines: 74500000 Processed lines: 72500000 Processed lines: 73500000 Finished processing child process. Exiting.. Processed lines: 73000000 Processed lines: 75500000 Processed lines: 75000000 Processed lines: 74500000 Processed lines: 76500000 Processed lines: 77000000 Processed lines: 72500000 Processed lines: 73000000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 73500000 Processed lines: 76000000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 77000000 Processed lines: 75000000 Processed lines: 73500000 Processed lines: 72500000 Processed lines: 73000000 Processed lines: 74000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 76500000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 73000000 Processed lines: 74000000 Finished processing child process. Exiting.. Processed lines: 73500000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 75000000 Processed lines: 77000000 Processed lines: 73000000 Processed lines: 76000000 Processed lines: 73500000 Processed lines: 76000000 Processed lines: 74000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 76000000 Processed lines: 75500000 Processed lines: 74000000 Processed lines: 73500000 Processed lines: 73000000 Processed lines: 74500000 Processed lines: 77000000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 75000000 Processed lines: 75500000 Processed lines: 73500000 Processed lines: 74500000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 74000000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 76500000 Processed lines: 73500000 Processed lines: 76500000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 77000000 Processed lines: 76500000 Processed lines: 76000000 Processed lines: 74500000 Processed lines: 75000000 Finished processing child process. Exiting.. Processed lines: 74000000 Processed lines: 73500000 Processed lines: 75500000 Processed lines: 76000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 75000000 Processed lines: 74000000 Processed lines: 76500000 Processed lines: 76000000 Processed lines: 74500000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 77000000 Processed lines: 76500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 75500000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 76000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 74500000 Processed lines: 77000000 Processed lines: 76500000 Processed lines: 75000000 Processed lines: 74500000 Processed lines: 75500000 Processed lines: 75000000 Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 75500000 Processed lines: 76000000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 76500000 Processed lines: 77000000 Finished processing child process. Exiting.. Processed lines: 76000000 Processed lines: 77000000 Processed lines: 75000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 75500000 Finished processing child process. Exiting.. Processed lines: 76000000 Processed lines: 75000000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 76000000 Processed lines: 76500000 Processed lines: 75000000 Processed lines: 75500000 Processed lines: 77000000 Processed lines: 76500000 Processed lines: 75500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 76000000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 75500000 Processed lines: 76000000 Processed lines: 76500000 Processed lines: 77000000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 77000000 Processed lines: 76000000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 76500000 Processed lines: 77000000 Processed lines: 76000000 Finished processing child process. Exiting.. Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 76500000 Processed lines: 76000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 76500000 Processed lines: 77000000 Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 77000000 Finished processing child process. Exiting.. Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76000000 Finished processing child process. Exiting.. Processed lines: 77000000 Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 76500000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 77000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 77191386 lines in total Total number of methylation call strings processed: 154382772 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1930776015 Total methylated C's in CpG context: 34183478 Total methylated C's in CHG context: 1349709 Total methylated C's in CHH context: 5922078 Total C to T conversions in CpG context: 220646744 Total C to T conversions in CHG context: 346999276 Total C to T conversions in CHH context: 1321674730 C methylated in CpG context: 13.4% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 31M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 31M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 31M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_31M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>35F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >35F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 35F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/35F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/35F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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lines: 77000000 Processed lines: 77000000 Processed lines: 81500000 Processed lines: 82500000 Processed lines: 76000000 Processed lines: 80000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 78500000 Processed lines: 79000000 Processed lines: 82000000 Processed lines: 80500000 Processed lines: 82500000 Processed lines: 78500000 Processed lines: 80500000 Processed lines: 83000000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 81500000 Processed lines: 82000000 Processed lines: 79500000 Processed lines: 75500000 Processed lines: 82500000 Processed lines: 80000000 Processed lines: 81500000 Processed lines: 80500000 Processed lines: 77500000 Processed lines: 77500000 Processed lines: 77500000 Processed lines: 82000000 Processed lines: 83000000 Processed lines: 77500000 Processed lines: 80500000 Processed lines: 79000000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 82500000 Processed lines: 81000000 Processed lines: 79500000 Processed lines: 83000000 Processed lines: 83500000 Processed lines: 81000000 Processed lines: 79000000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 82000000 Processed lines: 82500000 Processed lines: 83000000 Processed lines: 80000000 Processed lines: 80500000 Processed lines: 76000000 Processed lines: 82000000 Processed lines: 81000000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 78000000 Processed lines: 78000000 Processed lines: 78000000 Finished processing child process. Exiting.. Processed lines: 81000000 Processed lines: 78000000 Processed lines: 79500000 Processed lines: 83000000 Processed lines: 81500000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 80000000 Processed lines: 83500000 Processed lines: 81500000 Processed lines: 79500000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 82500000 Processed lines: 83000000 Finished processing child process. Exiting.. Processed lines: 83500000 Processed lines: 81000000 Processed lines: 80500000 Processed lines: 76500000 Processed lines: 82500000 Processed lines: 81500000 Finished processing child process. Exiting.. Processed lines: 83000000 Processed lines: 81500000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 83500000 Processed lines: 80000000 Processed lines: 82000000 Processed lines: 80500000 Processed lines: 77500000 Processed lines: 77500000 Processed lines: 77500000 Finished processing child process. Exiting.. Processed lines: 82000000 Processed lines: 81000000 Processed lines: 80000000 Processed lines: 83000000 Processed lines: 83500000 Processed lines: 81000000 Processed lines: 81500000 Processed lines: 81000000 Processed lines: 83000000 Processed lines: 77000000 Finished processing child process. Exiting.. Processed lines: 82000000 Processed lines: 83500000 Processed lines: 82000000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 82500000 Processed lines: 79000000 Processed lines: 80500000 Processed lines: 81000000 Finished processing child process. Exiting.. Processed lines: 78000000 Processed lines: 78000000 Processed lines: 78000000 Processed lines: 82500000 Processed lines: 81500000 Processed lines: 83500000 Processed lines: 80500000 Finished processing child process. Exiting.. Processed lines: 81500000 Processed lines: 82000000 Processed lines: 81500000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 77500000 Processed lines: 82500000 Processed lines: 83000000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 79500000 Finished processing child process. Exiting.. Processed lines: 81000000 Processed lines: 79500000 Processed lines: 81500000 Processed lines: 83000000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 82000000 Processed lines: 81000000 Finished processing child process. Exiting.. Processed lines: 82500000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 83000000 Processed lines: 78000000 Processed lines: 83500000 Processed lines: 81500000 Processed lines: 83000000 Processed lines: 82000000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 81500000 Processed lines: 83000000 Finished processing child process. Exiting.. Processed lines: 82500000 Processed lines: 82500000 Processed lines: 83500000 Finished processing child process. Exiting.. Processed lines: 78500000 Processed lines: 82000000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 83000000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 83500000 Processed lines: 83000000 Processed lines: 83000000 Finished processing child process. Exiting.. Processed lines: 82500000 Processed lines: 79000000 Processed lines: 83000000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 81000000 Finished processing child process. Exiting.. Processed lines: 83500000 Processed lines: 82500000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 83500000 Processed lines: 83500000 Finished processing child process. Exiting.. Processed lines: 83000000 Processed lines: 83500000 Processed lines: 79500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 83000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 80500000 Processed lines: 80500000 Processed lines: 80500000 Finished processing child process. Exiting.. Processed lines: 83500000 Processed lines: 80000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 83500000 Finished processing child process. Exiting.. Processed lines: 81000000 Processed lines: 81000000 Processed lines: 81000000 Finished processing child process. Exiting.. Processed lines: 80500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 81500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 82500000 Processed lines: 83000000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 83500000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 83853098 lines in total Total number of methylation call strings processed: 167706196 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2298440911 Total methylated C's in CpG context: 34690212 Total methylated C's in CHG context: 1663625 Total methylated C's in CHH context: 6720771 Total C to T conversions in CpG context: 254538680 Total C to T conversions in CHG context: 397912346 Total C to T conversions in CHH context: 1602915277 C methylated in CpG context: 12.0% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 35F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 35F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 35F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_35F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>36F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >36F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 36F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/36F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/36F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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Processed lines: 80500000 Processed lines: 79000000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 81000000 Processed lines: 83500000 Processed lines: 79500000 Processed lines: 82000000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 83000000 Processed lines: 80000000 Processed lines: 79000000 Processed lines: 82500000 Processed lines: 81000000 Processed lines: 80000000 Processed lines: 81500000 Processed lines: 80500000 Processed lines: 79500000 Processed lines: 82000000 Processed lines: 83500000 Processed lines: 78500000 Processed lines: 84500000 Processed lines: 79000000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 81000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 79500000 Processed lines: 84000000 Processed lines: 81500000 Processed lines: 80000000 Processed lines: 82500000 Processed lines: 83500000 Processed lines: 80500000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 79500000 Processed lines: 83000000 Processed lines: 81500000 Processed lines: 80500000 Processed lines: 82000000 Processed lines: 81000000 Processed lines: 80000000 Processed lines: 82500000 Processed lines: 84000000 Processed lines: 85000000 Processed lines: 79000000 Processed lines: 84000000 Processed lines: 83000000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 83500000 Processed lines: 81500000 Processed lines: 79500000 Processed lines: 83500000 Processed lines: 80000000 Processed lines: 84500000 Processed lines: 82000000 Processed lines: 80500000 Processed lines: 83000000 Processed lines: 84000000 Finished processing child process. Exiting.. Processed lines: 81000000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 83500000 Processed lines: 80000000 Processed lines: 82000000 Processed lines: 81000000 Processed lines: 82500000 Processed lines: 81500000 Processed lines: 83000000 Processed lines: 84500000 Processed lines: 80500000 Processed lines: 84500000 Processed lines: 83500000 Processed lines: 79500000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 84000000 Processed lines: 82000000 Processed lines: 84000000 Processed lines: 80000000 Processed lines: 85000000 Processed lines: 80500000 Processed lines: 82500000 Processed lines: 81000000 Processed lines: 84500000 Processed lines: 83500000 Processed lines: 81500000 Processed lines: 80000000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 84000000 Processed lines: 82500000 Processed lines: 80500000 Processed lines: 81500000 Processed lines: 83000000 Processed lines: 82000000 Processed lines: 85000000 Processed lines: 83500000 Processed lines: 81000000 Processed lines: 85000000 Processed lines: 84000000 Processed lines: 80000000 Processed lines: 84500000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 82500000 Processed lines: 84500000 Finished processing child process. Exiting.. Processed lines: 80500000 Processed lines: 83000000 Processed lines: 81000000 Processed lines: 85000000 Processed lines: 84000000 Processed lines: 81500000 Finished processing child process. Exiting.. Processed lines: 82000000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 84500000 Processed lines: 83000000 Processed lines: 81000000 Processed lines: 83500000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 82500000 Processed lines: 84000000 Processed lines: 81500000 Processed lines: 84500000 Processed lines: 85000000 Processed lines: 83000000 Processed lines: 85000000 Processed lines: 80500000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 83500000 Processed lines: 81500000 Processed lines: 84500000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 83500000 Processed lines: 84000000 Processed lines: 82500000 Processed lines: 81500000 Processed lines: 83000000 Processed lines: 84500000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 83500000 Processed lines: 81500000 Processed lines: 81000000 Processed lines: 81500000 Processed lines: 84000000 Processed lines: 82000000 Processed lines: 81500000 Processed lines: 85000000 Finished processing child process. Exiting.. Processed lines: 82500000 Processed lines: 83000000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 84000000 Processed lines: 84500000 Processed lines: 83000000 Finished processing child process. Exiting.. Processed lines: 82000000 Processed lines: 85000000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 84000000 Finished processing child process. Exiting.. Processed lines: 82000000 Processed lines: 82000000 Processed lines: 81500000 Processed lines: 84500000 Processed lines: 82500000 Processed lines: 82000000 Processed lines: 83000000 Processed lines: 83500000 Processed lines: 84500000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 85000000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 84000000 Processed lines: 83000000 Finished processing child process. Exiting.. Processed lines: 84500000 Processed lines: 82000000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 85000000 Processed lines: 83000000 Processed lines: 82500000 Processed lines: 83500000 Processed lines: 84000000 Processed lines: 85000000 Processed lines: 82500000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 84000000 Processed lines: 84500000 Processed lines: 83000000 Processed lines: 83500000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 82500000 Processed lines: 83500000 Processed lines: 83000000 Processed lines: 84000000 Finished processing child process. Exiting.. Processed lines: 84500000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 84500000 Processed lines: 85000000 Processed lines: 83500000 Processed lines: 84000000 Finished processing child process. Exiting.. Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83000000 Processed lines: 84000000 Processed lines: 83500000 Processed lines: 84500000 Processed lines: 85000000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 85000000 Processed lines: 84000000 Processed lines: 84500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 84000000 Processed lines: 84500000 Processed lines: 83500000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 85000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84500000 Processed lines: 85000000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 84500000 Processed lines: 84500000 Finished processing child process. Exiting.. Processed lines: 84000000 Processed lines: 84500000 Finished processing child process. Exiting.. Processed lines: 84500000 Processed lines: 84500000 Processed lines: 85000000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 84500000 Processed lines: 85000000 Processed lines: 85000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 85000000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 85207013 lines in total Total number of methylation call strings processed: 170414026 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2328124966 Total methylated C's in CpG context: 34436105 Total methylated C's in CHG context: 1741368 Total methylated C's in CHH context: 7322382 Total C to T conversions in CpG context: 260760867 Total C to T conversions in CHG context: 405189661 Total C to T conversions in CHH context: 1618674583 C methylated in CpG context: 11.7% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 36F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 36F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 36F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_36F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Finished BedGraph conversion ... Checking file >>39F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >39F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 39F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/39F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/39F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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Processed lines: 76500000 Processed lines: 75000000 Processed lines: 74000000 Processed lines: 78500000 Processed lines: 79000000 Processed lines: 80000000 Processed lines: 75500000 Processed lines: 77000000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 80000000 Processed lines: 77500000 Processed lines: 76500000 Processed lines: 74500000 Processed lines: 77500000 Processed lines: 74500000 Processed lines: 74500000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 78000000 Processed lines: 76000000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 76000000 Processed lines: 79500000 Processed lines: 80000000 Processed lines: 75500000 Processed lines: 77000000 Processed lines: 75000000 Finished processing child process. Exiting.. Processed lines: 79000000 Processed lines: 75500000 Processed lines: 74500000 Processed lines: 79500000 Processed lines: 77500000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 78000000 Processed lines: 77000000 Processed lines: 78000000 Processed lines: 80000000 Processed lines: 75000000 Processed lines: 78500000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 79000000 Processed lines: 79000000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 80000000 Processed lines: 76500000 Processed lines: 76000000 Processed lines: 77500000 Processed lines: 75500000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 76000000 Processed lines: 80000000 Processed lines: 75000000 Processed lines: 78000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 78500000 Processed lines: 76000000 Processed lines: 77500000 Processed lines: 78500000 Processed lines: 79000000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 77000000 Processed lines: 75500000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 77000000 Processed lines: 78000000 Processed lines: 76500000 Processed lines: 80000000 Processed lines: 76000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 78500000 Processed lines: 77000000 Processed lines: 79000000 Processed lines: 76500000 Processed lines: 78000000 Processed lines: 79000000 Processed lines: 79500000 Processed lines: 77500000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 80000000 Processed lines: 80000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 76000000 Processed lines: 76000000 Processed lines: 78500000 Processed lines: 77500000 Processed lines: 77000000 Processed lines: 76500000 Processed lines: 77000000 Processed lines: 76000000 Processed lines: 79000000 Processed lines: 77500000 Processed lines: 79500000 Processed lines: 78500000 Processed lines: 79500000 Processed lines: 77000000 Processed lines: 80000000 Processed lines: 78000000 Processed lines: 76500000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 79000000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 78000000 Processed lines: 77500000 Processed lines: 77000000 Processed lines: 77500000 Processed lines: 79500000 Processed lines: 76500000 Processed lines: 78000000 Processed lines: 80000000 Processed lines: 79000000 Processed lines: 80000000 Processed lines: 77500000 Finished processing child process. Exiting.. Processed lines: 78500000 Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 79500000 Processed lines: 78500000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 78000000 Processed lines: 77500000 Processed lines: 78000000 Processed lines: 80000000 Processed lines: 77000000 Processed lines: 78500000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 78000000 Processed lines: 79000000 Processed lines: 77500000 Processed lines: 77500000 Processed lines: 77500000 Processed lines: 80000000 Processed lines: 79000000 Processed lines: 77500000 Processed lines: 77500000 Processed lines: 78500000 Processed lines: 78000000 Finished processing child process. Exiting.. Processed lines: 78500000 Processed lines: 77500000 Processed lines: 79000000 Processed lines: 80000000 Processed lines: 78500000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 78000000 Processed lines: 78000000 Processed lines: 78000000 Processed lines: 79500000 Processed lines: 78000000 Processed lines: 78000000 Processed lines: 79000000 Processed lines: 78500000 Processed lines: 79000000 Processed lines: 79500000 Processed lines: 78000000 Processed lines: 79000000 Processed lines: 80000000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 78500000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 79500000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 79000000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 80000000 Processed lines: 78500000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 80000000 Processed lines: 79000000 Processed lines: 79500000 Processed lines: 79000000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 79000000 Finished processing child process. Exiting.. Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 80000000 Processed lines: 79500000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 80000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 80000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 80000000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 80072194 lines in total Total number of methylation call strings processed: 160144388 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2074276116 Total methylated C's in CpG context: 31536948 Total methylated C's in CHG context: 1496914 Total methylated C's in CHH context: 6317241 Total C to T conversions in CpG context: 233554310 Total C to T conversions in CHG context: 362416138 Total C to T conversions in CHH context: 1438954565 C methylated in CpG context: 11.9% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 39F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 39F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 39F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_39F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>3F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >3F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 3F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/3F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/3F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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lines: 83500000 Processed lines: 85000000 Processed lines: 82000000 Processed lines: 83000000 Finished processing child process. Exiting.. Processed lines: 81500000 Processed lines: 83500000 Processed lines: 82000000 Processed lines: 78000000 Processed lines: 86500000 Processed lines: 82500000 Processed lines: 85000000 Processed lines: 83500000 Processed lines: 80500000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 85500000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 86000000 Processed lines: 78500000 Processed lines: 80000000 Processed lines: 84500000 Processed lines: 85500000 Processed lines: 85000000 Processed lines: 86000000 Processed lines: 84000000 Processed lines: 83000000 Processed lines: 85500000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 84000000 Processed lines: 82000000 Processed lines: 82500000 Processed lines: 83000000 Processed lines: 84000000 Processed lines: 85500000 Processed lines: 78500000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 81000000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 86500000 Processed lines: 85500000 Processed lines: 86000000 Processed lines: 85000000 Processed lines: 79000000 Processed lines: 80500000 Finished processing child process. Exiting.. Processed lines: 86000000 Processed lines: 86500000 Processed lines: 85500000 Processed lines: 84500000 Processed lines: 83500000 Processed lines: 86000000 Finished processing child process. Exiting.. Processed lines: 84000000 Processed lines: 83000000 Processed lines: 84500000 Processed lines: 82500000 Processed lines: 83000000 Processed lines: 83500000 Processed lines: 84500000 Processed lines: 86000000 Processed lines: 79000000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 81500000 Processed lines: 86500000 Processed lines: 86000000 Processed lines: 85500000 Processed lines: 79500000 Processed lines: 81000000 Processed lines: 86500000 Finished processing child process. Exiting.. Processed lines: 86000000 Processed lines: 85000000 Processed lines: 86500000 Processed lines: 84000000 Finished processing child process. Exiting.. Processed lines: 84500000 Processed lines: 83500000 Processed lines: 85000000 Finished processing child process. Exiting.. Processed lines: 83000000 Processed lines: 83500000 Processed lines: 86500000 Processed lines: 84000000 Processed lines: 85000000 Processed lines: 86000000 Finished processing child process. Exiting.. Processed lines: 86500000 Processed lines: 79500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 82000000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 86500000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 85500000 Processed lines: 84500000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 84000000 Processed lines: 85500000 Processed lines: 83500000 Processed lines: 84000000 Processed lines: 84500000 Processed lines: 85500000 Processed lines: 86500000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 82000000 Processed lines: 82500000 Processed lines: 80000000 Processed lines: 86000000 Processed lines: 85000000 Processed lines: 80500000 Processed lines: 85500000 Processed lines: 84500000 Processed lines: 86000000 Processed lines: 84000000 Processed lines: 84500000 Processed lines: 85000000 Processed lines: 86000000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 86500000 Processed lines: 85500000 Processed lines: 80500000 Processed lines: 83000000 Processed lines: 86000000 Processed lines: 81000000 Processed lines: 85000000 Finished processing child process. Exiting.. Processed lines: 86500000 Processed lines: 84500000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 85500000 Processed lines: 86500000 Finished processing child process. Exiting.. Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 86000000 Processed lines: 86500000 Processed lines: 85500000 Processed lines: 81000000 Processed lines: 81500000 Processed lines: 83500000 Processed lines: 85000000 Finished processing child process. Exiting.. Processed lines: 86000000 Processed lines: 85500000 Processed lines: 83500000 Processed lines: 86500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 86000000 Finished processing child process. Exiting.. Processed lines: 85500000 Processed lines: 82000000 Processed lines: 81500000 Processed lines: 86000000 Processed lines: 84000000 Processed lines: 86500000 Finished processing child process. Exiting.. Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 86500000 Processed lines: 86000000 Finished processing child process. Exiting.. Processed lines: 86500000 Processed lines: 82500000 Processed lines: 84500000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 86500000 Finished processing child process. Exiting.. Processed lines: 82500000 Processed lines: 85000000 Processed lines: 83000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 83000000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 84500000 Processed lines: 84500000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86500000 Processed lines: 86500000 Now waiting for all child processes to complete Finished processing child process. Exiting.. Merging individual splitting reports into overall report: '3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 86601366 lines in total Total number of methylation call strings processed: 173202732 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2245598302 Total methylated C's in CpG context: 33278296 Total methylated C's in CHG context: 1581182 Total methylated C's in CHH context: 6889997 Total C to T conversions in CpG context: 258732657 Total C to T conversions in CHG context: 397571420 Total C to T conversions in CHH context: 1547544750 C methylated in CpG context: 11.4% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 3F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 3F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 3F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_3F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>41F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >41F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 41F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/41F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/41F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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lines: 51500000 Processed lines: 50000000 Processed lines: 53000000 Processed lines: 52500000 Processed lines: 51000000 Processed lines: 54000000 Processed lines: 50500000 Processed lines: 50500000 Processed lines: 53500000 Processed lines: 50000000 Processed lines: 54000000 Processed lines: 53500000 Processed lines: 52000000 Processed lines: 51500000 Processed lines: 52500000 Processed lines: 52500000 Processed lines: 50500000 Processed lines: 54500000 Processed lines: 51000000 Processed lines: 51000000 Processed lines: 50500000 Processed lines: 54000000 Processed lines: 53500000 Processed lines: 50500000 Processed lines: 52000000 Processed lines: 54500000 Processed lines: 51500000 Processed lines: 54000000 Processed lines: 52000000 Processed lines: 53500000 Processed lines: 53000000 Processed lines: 54500000 Processed lines: 50500000 Processed lines: 54000000 Processed lines: 51500000 Processed lines: 51000000 Processed lines: 51000000 Processed lines: 54500000 Processed lines: 50500000 Processed lines: 54000000 Processed lines: 52500000 Processed lines: 52000000 Processed lines: 55000000 Processed lines: 53000000 Processed lines: 53000000 Processed lines: 51000000 Processed lines: 54500000 Processed lines: 51500000 Processed lines: 51500000 Processed lines: 54000000 Processed lines: 55000000 Processed lines: 51000000 Processed lines: 52500000 Processed lines: 51000000 Processed lines: 54500000 Processed lines: 52000000 Processed lines: 55000000 Processed lines: 54000000 Processed lines: 53500000 Processed lines: 54500000 Processed lines: 52500000 Processed lines: 51000000 Processed lines: 52000000 Processed lines: 55000000 Processed lines: 51500000 Processed lines: 51500000 Processed lines: 54500000 Processed lines: 51000000 Processed lines: 53000000 Processed lines: 55500000 Processed lines: 52500000 Processed lines: 53500000 Processed lines: 53500000 Processed lines: 55000000 Processed lines: 51500000 Processed lines: 55500000 Processed lines: 54500000 Processed lines: 52000000 Processed lines: 52000000 Processed lines: 53000000 Processed lines: 51500000 Processed lines: 55000000 Processed lines: 51500000 Processed lines: 55500000 Processed lines: 52500000 Processed lines: 54500000 Processed lines: 54000000 Processed lines: 55000000 Processed lines: 53000000 Processed lines: 55500000 Processed lines: 52500000 Processed lines: 51500000 Processed lines: 52000000 Processed lines: 52000000 Processed lines: 55000000 Processed lines: 56000000 Processed lines: 53500000 Processed lines: 51500000 Finished processing child process. Exiting.. Processed lines: 53000000 Processed lines: 54000000 Processed lines: 54000000 Processed lines: 55500000 Processed lines: 56000000 Processed lines: 55000000 Processed lines: 52000000 Finished processing child process. Exiting.. Processed lines: 52500000 Processed lines: 52500000 Processed lines: 55500000 Processed lines: 53500000 Processed lines: 56000000 Processed lines: 52000000 Processed lines: 52000000 Processed lines: 55000000 Finished processing child process. Exiting.. Processed lines: 55500000 Processed lines: 54500000 Processed lines: 53000000 Processed lines: 56000000 Processed lines: 53500000 Finished processing child process. Exiting.. Processed lines: 53000000 Processed lines: 52000000 Processed lines: 55500000 Processed lines: 52500000 Processed lines: 52500000 Processed lines: 54000000 Processed lines: 52000000 Processed lines: 53500000 Processed lines: 56000000 Processed lines: 54500000 Processed lines: 54500000 Processed lines: 55500000 Finished processing child process. Exiting.. Processed lines: 52500000 Processed lines: 53000000 Processed lines: 56000000 Processed lines: 54000000 Processed lines: 53000000 Finished processing child process. Exiting.. Processed lines: 52500000 Processed lines: 55500000 Processed lines: 52500000 Processed lines: 56000000 Processed lines: 55000000 Processed lines: 53500000 Finished processing child process. Exiting.. Processed lines: 56000000 Processed lines: 54000000 Processed lines: 53500000 Processed lines: 52500000 Finished processing child process. Exiting.. Processed lines: 53000000 Processed lines: 53000000 Processed lines: 54500000 Processed lines: 52500000 Processed lines: 54000000 Processed lines: 55000000 Processed lines: 55000000 Processed lines: 56000000 Finished processing child process. Exiting.. Processed lines: 53000000 Processed lines: 53500000 Processed lines: 54500000 Processed lines: 53500000 Processed lines: 56000000 Processed lines: 55500000 Processed lines: 53000000 Processed lines: 53000000 Finished processing child process. Exiting.. Processed lines: 54000000 Processed lines: 54500000 Processed lines: 54000000 Processed lines: 53000000 Processed lines: 53500000 Processed lines: 53500000 Processed lines: 55000000 Processed lines: 54500000 Processed lines: 53000000 Processed lines: 55500000 Processed lines: 55500000 Processed lines: 55000000 Processed lines: 54000000 Processed lines: 53500000 Processed lines: 54000000 Processed lines: 56000000 Processed lines: 53500000 Processed lines: 53500000 Finished processing child process. Exiting.. Processed lines: 54500000 Processed lines: 55000000 Processed lines: 54500000 Processed lines: 53500000 Processed lines: 54000000 Processed lines: 55500000 Processed lines: 54000000 Processed lines: 55000000 Processed lines: 56000000 Processed lines: 56000000 Processed lines: 53500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 55500000 Processed lines: 54500000 Processed lines: 54000000 Processed lines: 54500000 Processed lines: 55000000 Processed lines: 54000000 Processed lines: 54000000 Processed lines: 55000000 Processed lines: 55500000 Processed lines: 54000000 Processed lines: 56000000 Processed lines: 54500000 Processed lines: 54500000 Finished processing child process. Exiting.. Processed lines: 55500000 Processed lines: 54000000 Processed lines: 56000000 Processed lines: 55000000 Processed lines: 54500000 Finished processing child process. Exiting.. Processed lines: 55000000 Processed lines: 55500000 Processed lines: 54500000 Processed lines: 54500000 Processed lines: 55500000 Processed lines: 56000000 Finished processing child process. Exiting.. Processed lines: 54500000 Processed lines: 55000000 Processed lines: 55000000 Processed lines: 56000000 Finished processing child process. Exiting.. Processed lines: 54500000 Processed lines: 55500000 Processed lines: 55000000 Processed lines: 55500000 Processed lines: 56000000 Processed lines: 55000000 Processed lines: 55000000 Finished processing child process. Exiting.. Processed lines: 56000000 Finished processing child process. Exiting.. Processed lines: 55000000 Processed lines: 55500000 Processed lines: 55500000 Processed lines: 55000000 Processed lines: 56000000 Processed lines: 55500000 Processed lines: 56000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 55500000 Processed lines: 55500000 Processed lines: 56000000 Processed lines: 56000000 Processed lines: 55500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 55500000 Processed lines: 56000000 Finished processing child process. Exiting.. Processed lines: 56000000 Processed lines: 56000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 56000000 Finished processing child process. Exiting.. Processed lines: 56000000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 56050400 lines in total Total number of methylation call strings processed: 112100800 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1402364113 Total methylated C's in CpG context: 21603092 Total methylated C's in CHG context: 1050441 Total methylated C's in CHH context: 4458912 Total C to T conversions in CpG context: 158790370 Total C to T conversions in CHG context: 248626025 Total C to T conversions in CHH context: 967835273 C methylated in CpG context: 12.0% C methylated in non-CpG context: 0.5% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 41F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 41F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 41F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_41F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Finished BedGraph conversion ... Checking file >>44F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >44F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 44F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/44F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/44F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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Processed lines: 137000000 Processed lines: 137000000 Processed lines: 137000000 Processed lines: 146500000 Processed lines: 137000000 Processed lines: 137000000 Processed lines: 137000000 Processed lines: 137000000 Processed lines: 144500000 Processed lines: 142500000 Processed lines: 145000000 Processed lines: 150000000 Processed lines: 150500000 Processed lines: 148000000 Processed lines: 131500000 Processed lines: 149000000 Processed lines: 151000000 Processed lines: 146000000 Processed lines: 149500000 Processed lines: 143000000 Processed lines: 144500000 Processed lines: 151000000 Processed lines: 148500000 Processed lines: 149000000 Processed lines: 147500000 Processed lines: 142500000 Processed lines: 143500000 Processed lines: 147000000 Processed lines: 145000000 Processed lines: 145500000 Processed lines: 143000000 Processed lines: 150500000 Processed lines: 140000000 Processed lines: 137500000 Processed lines: 137500000 Processed lines: 137500000 Processed lines: 137500000 Processed lines: 137500000 Processed lines: 137500000 Processed lines: 137500000 Processed lines: 151000000 Processed lines: 148500000 Processed lines: 149500000 Processed lines: 151500000 Processed lines: 146500000 Processed lines: 150000000 Processed lines: 132000000 Processed lines: 145000000 Processed lines: 151500000 Processed lines: 143500000 Processed lines: 149000000 Processed lines: 149500000 Processed lines: 148000000 Processed lines: 144000000 Processed lines: 143000000 Processed lines: 147500000 Processed lines: 145500000 Processed lines: 146000000 Processed lines: 143500000 Processed lines: 151000000 Processed lines: 151500000 Processed lines: 149000000 Processed lines: 138000000 Processed lines: 138000000 Processed lines: 138000000 Processed lines: 138000000 Processed lines: 138000000 Processed lines: 138000000 Processed lines: 138000000 Processed lines: 152000000 Processed lines: 150000000 Processed lines: 140500000 Processed lines: 147000000 Processed lines: 150500000 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Processed lines: 147000000 Processed lines: 143000000 Processed lines: 148500000 Processed lines: 149000000 Processed lines: 146000000 Processed lines: 155000000 Processed lines: 152000000 Processed lines: 153000000 Processed lines: 146500000 Processed lines: 153500000 Processed lines: 150000000 Processed lines: 155000000 Processed lines: 152500000 Processed lines: 135000000 Processed lines: 153000000 Processed lines: 148500000 Processed lines: 155000000 Processed lines: 151500000 Processed lines: 151000000 Processed lines: 154500000 Processed lines: 141000000 Processed lines: 141000000 Processed lines: 141000000 Processed lines: 141000000 Processed lines: 141000000 Processed lines: 141000000 Processed lines: 141000000 Processed lines: 147000000 Processed lines: 147500000 Processed lines: 155500000 Processed lines: 149000000 Processed lines: 149500000 Processed lines: 152500000 Processed lines: 146500000 Processed lines: 153500000 Processed lines: 147000000 Processed lines: 143500000 Processed lines: 154000000 Processed lines: 150500000 Processed lines: 155500000 Processed lines: 153000000 Processed lines: 153500000 Processed lines: 149000000 Processed lines: 135500000 Processed lines: 155500000 Processed lines: 152000000 Processed lines: 151500000 Processed lines: 155000000 Processed lines: 156000000 Processed lines: 147500000 Processed lines: 148000000 Processed lines: 149500000 Processed lines: 153000000 Processed lines: 154000000 Processed lines: 150000000 Processed lines: 141500000 Processed lines: 141500000 Processed lines: 141500000 Processed lines: 141500000 Processed lines: 141500000 Processed lines: 141500000 Processed lines: 141500000 Processed lines: 147000000 Processed lines: 147500000 Processed lines: 154500000 Processed lines: 156000000 Processed lines: 144000000 Processed lines: 151000000 Processed lines: 153500000 Processed lines: 154000000 Processed lines: 149500000 Processed lines: 156000000 Processed lines: 152500000 Processed lines: 152000000 Processed lines: 155500000 Processed lines: 136000000 Processed lines: 156500000 Processed lines: 153500000 Processed lines: 150000000 Processed lines: 148500000 Processed lines: 154500000 Processed lines: 148000000 Processed lines: 150500000 Processed lines: 147500000 Processed lines: 155000000 Processed lines: 142000000 Processed lines: 142000000 Processed lines: 142000000 Processed lines: 142000000 Processed lines: 142000000 Processed lines: 142000000 Processed lines: 142000000 Processed lines: 148000000 Processed lines: 156500000 Processed lines: 151500000 Processed lines: 154000000 Processed lines: 144500000 Processed lines: 154500000 Processed lines: 150000000 Processed lines: 156500000 Processed lines: 153000000 Processed lines: 156000000 Processed lines: 152500000 Processed lines: 157000000 Processed lines: 154000000 Processed lines: 155000000 Processed lines: 150500000 Processed lines: 149000000 Processed lines: 148500000 Processed lines: 151000000 Processed lines: 155500000 Processed lines: 136500000 Processed lines: 148000000 Finished processing child process. Exiting.. Processed lines: 157000000 Processed lines: 148500000 Processed lines: 152000000 Processed lines: 142500000 Processed lines: 142500000 Processed lines: 142500000 Processed lines: 142500000 Processed lines: 142500000 Processed lines: 142500000 Processed lines: 142500000 Processed lines: 154500000 Processed lines: 155000000 Finished processing child process. Exiting.. Processed lines: 150500000 Processed lines: 145000000 Processed lines: 157000000 Processed lines: 156500000 Processed lines: 153000000 Processed lines: 153500000 Finished processing child process. Exiting.. Processed lines: 154500000 Processed lines: 155500000 Processed lines: 151000000 Processed lines: 149000000 Processed lines: 149500000 Processed lines: 151500000 Processed lines: 156000000 Processed lines: 148500000 Processed lines: 152500000 Processed lines: 137000000 Processed lines: 155000000 Processed lines: 155500000 Processed lines: 149000000 Processed lines: 143000000 Processed lines: 143000000 Processed lines: 143000000 Processed lines: 143000000 Processed lines: 143000000 Processed lines: 143000000 Processed lines: 143000000 Processed lines: 151000000 Processed lines: 157000000 Processed lines: 153500000 Processed lines: 154000000 Processed lines: 145500000 Processed lines: 156000000 Processed lines: 155000000 Processed lines: 151500000 Finished processing child process. Exiting.. Processed lines: 149500000 Processed lines: 150000000 Processed lines: 152000000 Processed lines: 156500000 Processed lines: 149000000 Processed lines: 153000000 Processed lines: 156000000 Processed lines: 155500000 Processed lines: 137500000 Processed lines: 149500000 Processed lines: 151500000 Processed lines: 143500000 Processed lines: 143500000 Processed lines: 143500000 Processed lines: 143500000 Processed lines: 143500000 Processed lines: 143500000 Processed lines: 143500000 Processed lines: 154000000 Processed lines: 154500000 Processed lines: 156500000 Processed lines: 155500000 Processed lines: 152000000 Processed lines: 157000000 Processed lines: 152500000 Processed lines: 150000000 Processed lines: 146000000 Processed lines: 150500000 Processed lines: 149500000 Processed lines: 153500000 Finished processing child process. Exiting.. Processed lines: 156000000 Processed lines: 156500000 Processed lines: 152000000 Processed lines: 150000000 Processed lines: 154500000 Processed lines: 155000000 Processed lines: 157000000 Processed lines: 138000000 Processed lines: 156000000 Processed lines: 144000000 Processed lines: 144000000 Processed lines: 144000000 Processed lines: 144000000 Processed lines: 144000000 Processed lines: 144000000 Processed lines: 144000000 Processed lines: 152500000 Processed lines: 153000000 Processed lines: 150500000 Processed lines: 151000000 Finished processing child process. Exiting.. Processed lines: 150000000 Processed lines: 154000000 Processed lines: 146500000 Processed lines: 156500000 Processed lines: 157000000 Processed lines: 152500000 Finished processing child process. Exiting.. Processed lines: 155500000 Processed lines: 155000000 Processed lines: 150500000 Processed lines: 156500000 Processed lines: 153000000 Processed lines: 153500000 Processed lines: 151000000 Processed lines: 151500000 Processed lines: 144500000 Processed lines: 144500000 Processed lines: 144500000 Processed lines: 144500000 Processed lines: 144500000 Processed lines: 144500000 Processed lines: 138500000 Processed lines: 144500000 Processed lines: 154500000 Processed lines: 150500000 Processed lines: 157000000 Processed lines: 147000000 Finished processing child process. Exiting.. Processed lines: 153000000 Processed lines: 156000000 Processed lines: 155500000 Processed lines: 157000000 Processed lines: 151000000 Processed lines: 153500000 Processed lines: 154000000 Processed lines: 151500000 Processed lines: 152000000 Finished processing child process. Exiting.. Processed lines: 155000000 Processed lines: 151000000 Processed lines: 145000000 Processed lines: 145000000 Processed lines: 145000000 Processed lines: 145000000 Processed lines: 145000000 Processed lines: 145000000 Processed lines: 145000000 Processed lines: 139000000 Processed lines: 153500000 Processed lines: 156000000 Processed lines: 156500000 Processed lines: 147500000 Processed lines: 154000000 Processed lines: 154500000 Processed lines: 152000000 Processed lines: 151500000 Processed lines: 152500000 Processed lines: 155500000 Processed lines: 151500000 Processed lines: 154000000 Processed lines: 145500000 Processed lines: 145500000 Processed lines: 145500000 Processed lines: 145500000 Processed lines: 145500000 Processed lines: 145500000 Processed lines: 145500000 Processed lines: 156500000 Processed lines: 157000000 Processed lines: 139500000 Finished processing child process. Exiting.. Processed lines: 154500000 Processed lines: 155000000 Processed lines: 152500000 Processed lines: 152000000 Processed lines: 153000000 Processed lines: 148000000 Processed lines: 156000000 Processed lines: 152000000 Processed lines: 154500000 Processed lines: 157000000 Finished processing child process. Exiting.. Processed lines: 146000000 Processed lines: 146000000 Processed lines: 146000000 Processed lines: 146000000 Processed lines: 146000000 Processed lines: 146000000 Processed lines: 146000000 Processed lines: 155000000 Processed lines: 155500000 Processed lines: 140000000 Processed lines: 153000000 Processed lines: 152500000 Processed lines: 153500000 Processed lines: 156500000 Processed lines: 148500000 Processed lines: 155000000 Processed lines: 152500000 Processed lines: 156000000 Processed lines: 155500000 Processed lines: 153500000 Processed lines: 153000000 Processed lines: 154000000 Processed lines: 157000000 Processed lines: 146500000 Processed lines: 146500000 Processed lines: 146500000 Processed lines: 146500000 Processed lines: 146500000 Processed lines: 146500000 Processed lines: 146500000 Processed lines: 140500000 Finished processing child process. Exiting.. Processed lines: 155500000 Processed lines: 153000000 Processed lines: 149000000 Processed lines: 156500000 Processed lines: 156000000 Processed lines: 153500000 Processed lines: 154000000 Processed lines: 154500000 Processed lines: 147000000 Processed lines: 147000000 Processed lines: 147000000 Processed lines: 147000000 Processed lines: 147000000 Processed lines: 147000000 Processed lines: 147000000 Processed lines: 156000000 Processed lines: 141000000 Processed lines: 157000000 Processed lines: 153500000 Processed lines: 156500000 Processed lines: 154000000 Processed lines: 149500000 Processed lines: 154500000 Processed lines: 155000000 Finished processing child process. Exiting.. Processed lines: 156500000 Processed lines: 147500000 Processed lines: 147500000 Processed lines: 147500000 Processed lines: 147500000 Processed lines: 147500000 Processed lines: 147500000 Processed lines: 147500000 Processed lines: 141500000 Processed lines: 157000000 Processed lines: 154500000 Processed lines: 155000000 Processed lines: 155500000 Processed lines: 154000000 Finished processing child process. Exiting.. Processed lines: 150000000 Processed lines: 157000000 Finished processing child process. Exiting.. Processed lines: 155500000 Processed lines: 155000000 Processed lines: 156000000 Processed lines: 148000000 Processed lines: 148000000 Processed lines: 148000000 Processed lines: 148000000 Processed lines: 148000000 Processed lines: 148000000 Processed lines: 148000000 Processed lines: 142000000 Processed lines: 154500000 Processed lines: 150500000 Processed lines: 156000000 Processed lines: 155500000 Processed lines: 156500000 Processed lines: 155000000 Processed lines: 148500000 Processed lines: 148500000 Processed lines: 148500000 Processed lines: 148500000 Processed lines: 148500000 Processed lines: 148500000 Processed lines: 148500000 Processed lines: 142500000 Processed lines: 156500000 Processed lines: 151000000 Processed lines: 157000000 Processed lines: 156000000 Finished processing child process. Exiting.. Processed lines: 155500000 Processed lines: 149000000 Processed lines: 149000000 Processed lines: 149000000 Processed lines: 149000000 Processed lines: 149000000 Processed lines: 149000000 Processed lines: 149000000 Processed lines: 143000000 Processed lines: 157000000 Processed lines: 156500000 Finished processing child process. Exiting.. Processed lines: 151500000 Processed lines: 149500000 Processed lines: 149500000 Processed lines: 149500000 Processed lines: 149500000 Processed lines: 149500000 Processed lines: 149500000 Processed lines: 149500000 Processed lines: 156000000 Processed lines: 157000000 Processed lines: 143500000 Finished processing child process. Exiting.. Processed lines: 152000000 Processed lines: 150000000 Processed lines: 150000000 Processed lines: 150000000 Processed lines: 150000000 Processed lines: 150000000 Processed lines: 150000000 Processed lines: 150000000 Processed lines: 156500000 Processed lines: 144000000 Processed lines: 150500000 Processed lines: 150500000 Processed lines: 150500000 Processed lines: 150500000 Processed lines: 150500000 Processed lines: 150500000 Processed lines: 150500000 Processed lines: 152500000 Processed lines: 157000000 Finished processing child process. Exiting.. Processed lines: 151000000 Processed lines: 151000000 Processed lines: 151000000 Processed lines: 151000000 Processed lines: 144500000 Processed lines: 151000000 Processed lines: 151000000 Processed lines: 151000000 Processed lines: 153000000 Processed lines: 151500000 Processed lines: 151500000 Processed lines: 151500000 Processed lines: 151500000 Processed lines: 151500000 Processed lines: 151500000 Processed lines: 151500000 Processed lines: 145000000 Processed lines: 153500000 Processed lines: 152000000 Processed lines: 152000000 Processed lines: 152000000 Processed lines: 152000000 Processed lines: 152000000 Processed lines: 152000000 Processed lines: 152000000 Processed lines: 145500000 Processed lines: 152500000 Processed lines: 152500000 Processed lines: 152500000 Processed lines: 154000000 Processed lines: 152500000 Processed lines: 152500000 Processed lines: 152500000 Processed lines: 152500000 Processed lines: 153000000 Processed lines: 146000000 Processed lines: 154500000 Processed lines: 153000000 Processed lines: 153000000 Processed lines: 153000000 Processed lines: 153000000 Processed lines: 153000000 Processed lines: 153000000 Processed lines: 153500000 Processed lines: 146500000 Processed lines: 155000000 Processed lines: 153500000 Processed lines: 153500000 Processed lines: 153500000 Processed lines: 153500000 Processed lines: 153500000 Processed lines: 154000000 Processed lines: 153500000 Processed lines: 154500000 Processed lines: 147000000 Processed lines: 155500000 Processed lines: 154000000 Processed lines: 154000000 Processed lines: 154000000 Processed lines: 154000000 Processed lines: 154000000 Processed lines: 154000000 Processed lines: 155000000 Processed lines: 147500000 Processed lines: 156000000 Processed lines: 154500000 Processed lines: 154500000 Processed lines: 154500000 Processed lines: 154500000 Processed lines: 154500000 Processed lines: 154500000 Processed lines: 155500000 Processed lines: 148000000 Processed lines: 155000000 Processed lines: 155000000 Processed lines: 155000000 Processed lines: 155000000 Processed lines: 155000000 Processed lines: 155000000 Processed lines: 156500000 Processed lines: 156000000 Processed lines: 156500000 Processed lines: 148500000 Processed lines: 155500000 Processed lines: 155500000 Processed lines: 155500000 Processed lines: 155500000 Processed lines: 155500000 Processed lines: 155500000 Processed lines: 157000000 Finished processing child process. Exiting.. Processed lines: 157000000 Finished processing child process. Exiting.. Processed lines: 149000000 Processed lines: 156000000 Processed lines: 156000000 Processed lines: 156000000 Processed lines: 156000000 Processed lines: 156000000 Processed lines: 156000000 Processed lines: 149500000 Processed lines: 156500000 Processed lines: 156500000 Processed lines: 156500000 Processed lines: 156500000 Processed lines: 156500000 Processed lines: 156500000 Processed lines: 150000000 Processed lines: 157000000 Processed lines: 157000000 Processed lines: 157000000 Processed lines: 157000000 Processed lines: 157000000 Processed lines: 157000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 150500000 Processed lines: 151000000 Processed lines: 151500000 Processed lines: 152000000 Processed lines: 152500000 Processed lines: 153000000 Processed lines: 153500000 Processed lines: 154000000 Processed lines: 154500000 Processed lines: 155000000 Processed lines: 155500000 Processed lines: 156000000 Processed lines: 156500000 Processed lines: 157000000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 157156371 lines in total Total number of methylation call strings processed: 314312742 Final Cytosine Methylation Report ================================= Total number of C's analysed: 4359634563 Total methylated C's in CpG context: 62164671 Total methylated C's in CHG context: 2974806 Total methylated C's in CHH context: 12301418 Total C to T conversions in CpG context: 486787004 Total C to T conversions in CHG context: 754995811 Total C to T conversions in CHH context: 3040410853 C methylated in CpG context: 11.3% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 44F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 44F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 44F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_44F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>48M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >48M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/48M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/48M_R2_val_2.fq.gz" Now reading in Bismark result file 48M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. 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97500000 Processed lines: 100000000 Processed lines: 94500000 Processed lines: 94500000 Processed lines: 98000000 Processed lines: 101500000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 100500000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 98500000 Processed lines: 96500000 Processed lines: 99000000 Processed lines: 102000000 Processed lines: 100000000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 101500000 Processed lines: 102000000 Processed lines: 100500000 Processed lines: 100000000 Processed lines: 99500000 Processed lines: 99000000 Processed lines: 98000000 Processed lines: 98500000 Processed lines: 100000000 Processed lines: 98000000 Processed lines: 102000000 Processed lines: 100500000 Processed lines: 101000000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 98500000 Processed lines: 99000000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 102500000 Processed lines: 99500000 Processed lines: 97000000 Processed lines: 100500000 Processed lines: 102000000 Processed lines: 101000000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 100500000 Processed lines: 102500000 Processed lines: 100000000 Processed lines: 99500000 Processed lines: 98500000 Processed lines: 100500000 Processed lines: 99000000 Processed lines: 102500000 Processed lines: 98500000 Processed lines: 101500000 Processed lines: 101000000 Processed lines: 99000000 Processed lines: 99500000 Processed lines: 96000000 Processed lines: 96000000 Processed lines: 103000000 Processed lines: 96000000 Processed lines: 96000000 Processed lines: 95500000 Processed lines: 95500000 Processed lines: 96000000 Processed lines: 100000000 Processed lines: 101000000 Processed lines: 102500000 Processed lines: 97500000 Processed lines: 101500000 Processed lines: 101000000 Processed lines: 96000000 Processed lines: 96000000 Processed lines: 103000000 Processed lines: 100500000 Processed lines: 100000000 Processed lines: 99000000 Processed lines: 101000000 Processed lines: 99500000 Processed lines: 103000000 Processed lines: 99000000 Processed lines: 102000000 Processed lines: 101500000 Processed lines: 99500000 Processed lines: 103500000 Processed lines: 100000000 Processed lines: 96500000 Processed lines: 96500000 Processed lines: 96500000 Finished processing child process. Exiting.. Processed lines: 96500000 Processed lines: 100500000 Processed lines: 96000000 Processed lines: 96000000 Processed lines: 96500000 Processed lines: 101500000 Processed lines: 103000000 Processed lines: 102000000 Processed lines: 98000000 Processed lines: 101500000 Processed lines: 101000000 Processed lines: 100500000 Processed lines: 96500000 Processed lines: 96500000 Processed lines: 103500000 Processed lines: 99500000 Finished processing child process. Exiting.. Processed lines: 101500000 Processed lines: 103500000 Processed lines: 100000000 Processed lines: 102500000 Finished processing child process. Exiting.. Processed lines: 99500000 Processed lines: 102000000 Processed lines: 100000000 Processed lines: 100500000 Processed lines: 101000000 Processed lines: 102000000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 103500000 Processed lines: 96500000 Processed lines: 97000000 Processed lines: 96500000 Processed lines: 102500000 Finished processing child process. Exiting.. Processed lines: 102000000 Processed lines: 98500000 Processed lines: 101500000 Processed lines: 101000000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 100000000 Processed lines: 102000000 Processed lines: 100500000 Processed lines: 103000000 Processed lines: 100000000 Processed lines: 102500000 Processed lines: 101000000 Processed lines: 100500000 Processed lines: 101500000 Processed lines: 102500000 Processed lines: 97500000 Processed lines: 97500000 Processed lines: 97500000 Processed lines: 97500000 Processed lines: 103000000 Processed lines: 97500000 Processed lines: 97000000 Processed lines: 97000000 Processed lines: 102500000 Processed lines: 102000000 Processed lines: 101500000 Processed lines: 99000000 Processed lines: 100500000 Processed lines: 97500000 Processed lines: 97500000 Processed lines: 102500000 Processed lines: 101000000 Processed lines: 103500000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 103000000 Processed lines: 101500000 Processed lines: 101000000 Processed lines: 102000000 Processed lines: 103500000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 103000000 Processed lines: 98000000 Processed lines: 98000000 Finished processing child process. Exiting.. Processed lines: 97500000 Processed lines: 97500000 Processed lines: 103000000 Processed lines: 102500000 Processed lines: 102000000 Processed lines: 101000000 Processed lines: 99500000 Processed lines: 103000000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 101500000 Processed lines: 101000000 Processed lines: 103500000 Processed lines: 102000000 Finished processing child process. Exiting.. Processed lines: 101500000 Processed lines: 102500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 98500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 98000000 Processed lines: 98000000 Processed lines: 103000000 Processed lines: 102500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 101500000 Processed lines: 103500000 Processed lines: 100000000 Finished processing child process. Exiting.. Processed lines: 98500000 Processed lines: 98500000 Processed lines: 102000000 Processed lines: 101500000 Processed lines: 102500000 Processed lines: 103000000 Processed lines: 102000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 103500000 Processed lines: 103000000 Processed lines: 98500000 Processed lines: 98500000 Finished processing child process. Exiting.. Processed lines: 102000000 Processed lines: 100500000 Processed lines: 102500000 Processed lines: 99000000 Processed lines: 99000000 Processed lines: 102000000 Processed lines: 103000000 Processed lines: 103500000 Processed lines: 102500000 Finished processing child process. Exiting.. Processed lines: 103500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 99500000 Finished processing child process. Exiting.. Processed lines: 99000000 Processed lines: 99000000 Processed lines: 102500000 Processed lines: 103000000 Processed lines: 101000000 Processed lines: 102500000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 103500000 Finished processing child process. Exiting.. Processed lines: 103000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 100000000 Processed lines: 103000000 Processed lines: 99500000 Processed lines: 99500000 Processed lines: 103500000 Finished processing child process. Exiting.. Processed lines: 101500000 Processed lines: 103000000 Processed lines: 103500000 Processed lines: 100000000 Processed lines: 100000000 Finished processing child process. Exiting.. Processed lines: 103500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Finished processing child process. Exiting.. Processed lines: 100000000 Processed lines: 100000000 Processed lines: 102000000 Processed lines: 103500000 Finished processing child process. Exiting.. Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 100500000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 102500000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101000000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 103000000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 101500000 Processed lines: 103500000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Finished processing child process. Exiting.. Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102000000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 102500000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103000000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Processed lines: 103500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Finished processing child process. Exiting.. Merging individual splitting reports into overall report: '48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 103543900 lines in total Total number of methylation call strings processed: 207087800 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2570349030 Total methylated C's in CpG context: 46430852 Total methylated C's in CHG context: 1955189 Total methylated C's in CHH context: 9333350 Total C to T conversions in CpG context: 302893637 Total C to T conversions in CHG context: 487842570 Total C to T conversions in CHH context: 1721893432 C methylated in CpG context: 13.3% C methylated in non-CpG context: 0.5% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 48M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 48M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 48M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_48M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>50F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >50F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 50F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/50F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/50F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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73000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 74000000 Processed lines: 75000000 Processed lines: 72000000 Processed lines: 75500000 Processed lines: 74500000 Processed lines: 76000000 Processed lines: 72000000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 70000000 Processed lines: 75000000 Processed lines: 70000000 Processed lines: 69500000 Processed lines: 73000000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 74000000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 73500000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 74500000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 75500000 Processed lines: 72500000 Processed lines: 76000000 Processed lines: 75000000 Processed lines: 76500000 Processed lines: 73500000 Processed lines: 72500000 Processed lines: 75500000 Processed lines: 73000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 73500000 Processed lines: 74500000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 70000000 Processed lines: 74000000 Processed lines: 70000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 75000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 76000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 76500000 Processed lines: 73000000 Processed lines: 75500000 Processed lines: 76000000 Processed lines: 74000000 Processed lines: 73000000 Processed lines: 73500000 Finished processing child process. Exiting.. Processed lines: 71000000 Processed lines: 71000000 Processed lines: 75000000 Processed lines: 74000000 Processed lines: 73500000 Processed lines: 74500000 Processed lines: 73000000 Processed lines: 73500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 75500000 Processed lines: 76500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 76000000 Processed lines: 73500000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 74500000 Processed lines: 73500000 Processed lines: 74000000 Processed lines: 75500000 Processed lines: 71500000 Processed lines: 75000000 Processed lines: 74500000 Processed lines: 71500000 Processed lines: 74000000 Processed lines: 73500000 Processed lines: 74000000 Finished processing child process. Exiting.. Processed lines: 71000000 Processed lines: 76000000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 76500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 74000000 Processed lines: 75000000 Processed lines: 74000000 Processed lines: 74500000 Finished processing child process. Exiting.. Processed lines: 76000000 Processed lines: 75500000 Processed lines: 75000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 76500000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 74500000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 75000000 Processed lines: 74500000 Processed lines: 76500000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 74500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 72000000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 75000000 Processed lines: 76500000 Processed lines: 76000000 Processed lines: 75000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 74000000 Processed lines: 74000000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 72500000 Processed lines: 75500000 Processed lines: 76500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 73000000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 76000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 76500000 Processed lines: 76000000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 76000000 Finished processing child process. Exiting.. Processed lines: 74000000 Processed lines: 74000000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 76500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 74500000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 74500000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 75500000 Processed lines: 74000000 Processed lines: 74000000 Finished processing child process. Exiting.. Processed lines: 74500000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 75000000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75000000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 75000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 75500000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76000000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 76500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 76500000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 76500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 76725340 lines in total Total number of methylation call strings processed: 153450680 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2234334784 Total methylated C's in CpG context: 33203460 Total methylated C's in CHG context: 1600748 Total methylated C's in CHH context: 6728111 Total C to T conversions in CpG context: 244518398 Total C to T conversions in CHG context: 386893249 Total C to T conversions in CHH context: 1561390818 C methylated in CpG context: 12.0% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 50F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 50F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 50F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_50F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>52F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >52F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 52F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/52F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/52F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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lines: 88000000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 87500000 Processed lines: 84500000 Processed lines: 89000000 Processed lines: 86000000 Processed lines: 87000000 Processed lines: 88000000 Processed lines: 86500000 Processed lines: 81500000 Processed lines: 84000000 Processed lines: 80500000 Processed lines: 85500000 Processed lines: 84500000 Processed lines: 87500000 Processed lines: 88500000 Processed lines: 89000000 Processed lines: 89500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 88500000 Processed lines: 87000000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 88000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 85000000 Processed lines: 89500000 Processed lines: 86500000 Processed lines: 87500000 Processed lines: 88500000 Processed lines: 87000000 Processed lines: 86000000 Processed lines: 84500000 Processed lines: 88000000 Processed lines: 82000000 Processed lines: 85000000 Processed lines: 81000000 Processed lines: 89000000 Processed lines: 89500000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 89000000 Processed lines: 87500000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 88500000 Processed lines: 90000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 86500000 Processed lines: 86500000 Processed lines: 85500000 Processed lines: 88000000 Processed lines: 87000000 Processed lines: 89000000 Processed lines: 87500000 Processed lines: 86500000 Processed lines: 88500000 Processed lines: 85000000 Processed lines: 82500000 Processed lines: 85500000 Processed lines: 89500000 Processed lines: 90000000 Processed lines: 81500000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 88000000 Processed lines: 89000000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 88500000 Processed lines: 87500000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 86000000 Processed lines: 89500000 Processed lines: 88000000 Processed lines: 89000000 Processed lines: 87000000 Processed lines: 85500000 Processed lines: 86000000 Processed lines: 83000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 90000000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 88500000 Processed lines: 89500000 Processed lines: 89000000 Processed lines: 88000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 86500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 90000000 Processed lines: 83000000 Processed lines: 83000000 Finished processing child process. Exiting.. Processed lines: 88500000 Processed lines: 89500000 Processed lines: 87500000 Processed lines: 86000000 Processed lines: 86500000 Processed lines: 83500000 Processed lines: 82500000 Processed lines: 89000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 88500000 Processed lines: 87000000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 89000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 88000000 Processed lines: 86500000 Processed lines: 87000000 Processed lines: 84000000 Processed lines: 89500000 Processed lines: 83000000 Processed lines: 90000000 Processed lines: 89000000 Finished processing child process. Exiting.. Processed lines: 87500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 89500000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 88500000 Processed lines: 87000000 Processed lines: 87500000 Processed lines: 84500000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 83500000 Processed lines: 89500000 Processed lines: 88000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 89000000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 87500000 Processed lines: 88000000 Processed lines: 85000000 Processed lines: 84000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 88500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 89500000 Processed lines: 88000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 88500000 Processed lines: 85500000 Processed lines: 84500000 Processed lines: 89000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 90000000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 89000000 Processed lines: 85500000 Processed lines: 89500000 Processed lines: 86000000 Processed lines: 85000000 Processed lines: 89000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 89500000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 86500000 Processed lines: 85500000 Processed lines: 89500000 Processed lines: 90000000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Finished processing child process. Exiting.. Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87000000 Processed lines: 86000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 87500000 Processed lines: 86500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 87000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 87500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 88000000 Processed lines: 88500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 90000000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 90021414 lines in total Total number of methylation call strings processed: 180042828 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2568366686 Total methylated C's in CpG context: 37267533 Total methylated C's in CHG context: 1750935 Total methylated C's in CHH context: 7241846 Total C to T conversions in CpG context: 280880651 Total C to T conversions in CHG context: 440074373 Total C to T conversions in CHH context: 1801151348 C methylated in CpG context: 11.7% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 52F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 52F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 52F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_52F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Finished BedGraph conversion ... Checking file >>53F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >53F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 53F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/53F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/53F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 82500000 Processed lines: 87000000 Processed lines: 84500000 Processed lines: 89500000 Processed lines: 86500000 Processed lines: 82000000 Processed lines: 87500000 Processed lines: 85500000 Processed lines: 88000000 Processed lines: 87500000 Processed lines: 89000000 Processed lines: 85500000 Processed lines: 87500000 Processed lines: 87000000 Processed lines: 86000000 Processed lines: 88500000 Processed lines: 90000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 87500000 Processed lines: 81500000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 89500000 Processed lines: 84000000 Processed lines: 87500000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 83000000 Processed lines: 90000000 Processed lines: 85000000 Processed lines: 87000000 Processed lines: 88000000 Processed lines: 86000000 Processed lines: 82500000 Processed lines: 88500000 Processed lines: 88000000 Processed lines: 89500000 Processed lines: 86000000 Processed lines: 88000000 Processed lines: 89000000 Processed lines: 86500000 Finished processing child process. Exiting.. Processed lines: 87500000 Processed lines: 88000000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 90000000 Processed lines: 82000000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 88000000 Finished processing child process. Exiting.. Processed lines: 83500000 Processed lines: 83500000 Processed lines: 85500000 Processed lines: 83500000 Processed lines: 83500000 Processed lines: 87500000 Processed lines: 88500000 Processed lines: 86500000 Processed lines: 89000000 Processed lines: 90000000 Processed lines: 88500000 Processed lines: 83000000 Processed lines: 86500000 Processed lines: 89500000 Processed lines: 88000000 Processed lines: 88500000 Processed lines: 87000000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 82500000 Processed lines: 88500000 Processed lines: 88000000 Processed lines: 89000000 Processed lines: 86000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 84000000 Processed lines: 87000000 Processed lines: 89500000 Finished processing child process. Exiting.. Processed lines: 89000000 Processed lines: 83500000 Processed lines: 87000000 Processed lines: 90000000 Processed lines: 88500000 Processed lines: 89000000 Processed lines: 87500000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 83000000 Processed lines: 88500000 Processed lines: 89500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 84500000 Processed lines: 86500000 Processed lines: 84500000 Processed lines: 90000000 Processed lines: 87500000 Finished processing child process. Exiting.. Processed lines: 87500000 Processed lines: 89500000 Processed lines: 89000000 Processed lines: 89500000 Processed lines: 84000000 Processed lines: 88000000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 90000000 Processed lines: 89000000 Finished processing child process. Exiting.. Processed lines: 83500000 Processed lines: 85000000 Processed lines: 85000000 Processed lines: 88000000 Processed lines: 85000000 Processed lines: 87000000 Processed lines: 85000000 Processed lines: 88000000 Processed lines: 89500000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 88500000 Processed lines: 84500000 Processed lines: 90000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 88500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 85500000 Processed lines: 87500000 Processed lines: 84000000 Processed lines: 88500000 Processed lines: 90000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 89000000 Finished processing child process. Exiting.. Processed lines: 85000000 Finished processing child process. Exiting.. Processed lines: 90000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 89000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 86000000 Processed lines: 84500000 Processed lines: 88000000 Finished processing child process. Exiting.. Processed lines: 89000000 Processed lines: 89500000 Processed lines: 85500000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 86500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 85000000 Processed lines: 90000000 Processed lines: 88500000 Processed lines: 89500000 Processed lines: 86000000 Processed lines: 90000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Finished processing child process. Exiting.. Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 85500000 Processed lines: 89000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 86500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 86000000 Processed lines: 89500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 87000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 90000000 Processed lines: 86500000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 87500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 87000000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 88000000 Processed lines: 87500000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 88000000 Processed lines: 88500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 88500000 Processed lines: 89000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 89000000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 90000000 Processed lines: 90000000 Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 90488807 lines in total Total number of methylation call strings processed: 180977614 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2495589632 Total methylated C's in CpG context: 36895767 Total methylated C's in CHG context: 1730940 Total methylated C's in CHH context: 7042696 Total C to T conversions in CpG context: 273790381 Total C to T conversions in CHG context: 429246884 Total C to T conversions in CHH context: 1746882964 C methylated in CpG context: 11.9% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 53F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 53F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 53F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_53F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>54F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >54F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 54F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/54F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/54F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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Exiting.. Processed lines: 92500000 Processed lines: 90000000 Processed lines: 93500000 Processed lines: 91000000 Processed lines: 92000000 Processed lines: 94000000 Processed lines: 90500000 Processed lines: 89000000 Finished processing child process. Exiting.. Processed lines: 91500000 Processed lines: 90000000 Processed lines: 89500000 Processed lines: 93500000 Processed lines: 86500000 Processed lines: 88000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 87000000 Processed lines: 90500000 Processed lines: 92500000 Processed lines: 88500000 Processed lines: 89500000 Processed lines: 92000000 Processed lines: 93500000 Processed lines: 89000000 Processed lines: 88500000 Processed lines: 91500000 Processed lines: 93000000 Processed lines: 94000000 Processed lines: 90500000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 91000000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 92000000 Processed lines: 90500000 Processed lines: 94000000 Processed lines: 90000000 Processed lines: 87000000 Processed lines: 93000000 Processed lines: 88500000 Processed lines: 89000000 Processed lines: 91000000 Processed lines: 92500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 90000000 Processed lines: 94000000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 89000000 Processed lines: 93500000 Processed lines: 92000000 Processed lines: 91000000 Processed lines: 93000000 Processed lines: 91500000 Processed lines: 92500000 Finished processing child process. Exiting.. Processed lines: 90000000 Processed lines: 92000000 Processed lines: 91000000 Processed lines: 90500000 Processed lines: 93500000 Processed lines: 87500000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 93000000 Processed lines: 89000000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 94000000 Processed lines: 90000000 Processed lines: 92500000 Processed lines: 89500000 Processed lines: 91500000 Processed lines: 93500000 Processed lines: 92000000 Processed lines: 93000000 Processed lines: 90500000 Processed lines: 91500000 Processed lines: 92500000 Processed lines: 91000000 Processed lines: 94000000 Processed lines: 92000000 Processed lines: 93500000 Processed lines: 90000000 Processed lines: 88000000 Processed lines: 89500000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 93000000 Processed lines: 90500000 Processed lines: 90000000 Processed lines: 92000000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 94000000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 92000000 Finished processing child process. Exiting.. Processed lines: 91500000 Processed lines: 91000000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 92500000 Processed lines: 90500000 Processed lines: 88500000 Processed lines: 91500000 Processed lines: 90000000 Processed lines: 93500000 Processed lines: 91000000 Processed lines: 92500000 Processed lines: 90500000 Finished processing child process. Exiting.. Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 92500000 Processed lines: 92000000 Finished processing child process. Exiting.. Processed lines: 91500000 Processed lines: 93000000 Processed lines: 91000000 Processed lines: 92000000 Processed lines: 89000000 Processed lines: 93500000 Processed lines: 94000000 Processed lines: 91500000 Processed lines: 93000000 Processed lines: 91000000 Processed lines: 90500000 Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 93000000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 91500000 Processed lines: 92500000 Processed lines: 92000000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 94000000 Processed lines: 93500000 Processed lines: 92000000 Processed lines: 91500000 Processed lines: 91000000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 93500000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 94000000 Processed lines: 92000000 Processed lines: 93000000 Processed lines: 92500000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 92500000 Processed lines: 92000000 Finished processing child process. Exiting.. Processed lines: 91500000 Processed lines: 93500000 Processed lines: 94000000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 90500000 Processed lines: 90500000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 93000000 Processed lines: 90500000 Finished processing child process. Exiting.. Processed lines: 93000000 Processed lines: 92500000 Processed lines: 92000000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 93000000 Processed lines: 94000000 Processed lines: 93500000 Processed lines: 91000000 Processed lines: 91000000 Processed lines: 91000000 Processed lines: 91000000 Processed lines: 93500000 Processed lines: 93000000 Processed lines: 92500000 Finished processing child process. Exiting.. Processed lines: 93500000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 94000000 Processed lines: 93500000 Processed lines: 91500000 Processed lines: 91500000 Processed lines: 91500000 Processed lines: 91500000 Processed lines: 93000000 Finished processing child process. Exiting.. Processed lines: 94000000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 92000000 Processed lines: 92000000 Processed lines: 92000000 Processed lines: 92000000 Processed lines: 93500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 92500000 Processed lines: 92500000 Processed lines: 92500000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 93000000 Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 93500000 Processed lines: 93500000 Processed lines: 93500000 Processed lines: 94000000 Processed lines: 94000000 Processed lines: 94000000 Processed lines: 94000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 94455697 lines in total Total number of methylation call strings processed: 188911394 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2648996482 Total methylated C's in CpG context: 39062781 Total methylated C's in CHG context: 1817935 Total methylated C's in CHH context: 7450923 Total C to T conversions in CpG context: 291237285 Total C to T conversions in CHG context: 459726104 Total C to T conversions in CHH context: 1849701454 C methylated in CpG context: 11.8% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 54F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 54F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 54F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_54F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>59M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >59M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 59M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/59M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/59M_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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Processed lines: 68500000 Processed lines: 67500000 Processed lines: 67500000 Processed lines: 71000000 Processed lines: 68000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 70000000 Processed lines: 70500000 Processed lines: 67500000 Processed lines: 69000000 Processed lines: 71500000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 70000000 Processed lines: 69000000 Processed lines: 72000000 Processed lines: 71000000 Processed lines: 71500000 Processed lines: 69500000 Processed lines: 72500000 Processed lines: 68000000 Processed lines: 68500000 Processed lines: 68000000 Processed lines: 69500000 Processed lines: 73000000 Processed lines: 70500000 Processed lines: 68500000 Processed lines: 69000000 Processed lines: 71500000 Processed lines: 70500000 Processed lines: 68000000 Processed lines: 68000000 Processed lines: 68500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 68000000 Processed lines: 69500000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 71500000 Processed lines: 71500000 Processed lines: 70500000 Processed lines: 72500000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 71500000 Processed lines: 70000000 Processed lines: 73000000 Processed lines: 68500000 Processed lines: 69000000 Processed lines: 68500000 Processed lines: 70000000 Processed lines: 71000000 Processed lines: 69000000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 69500000 Processed lines: 71000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 69000000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 71500000 Processed lines: 70000000 Processed lines: 68500000 Processed lines: 72000000 Processed lines: 72500000 Processed lines: 72000000 Processed lines: 71000000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 70000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 70500000 Processed lines: 72000000 Processed lines: 69500000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 71500000 Processed lines: 70500000 Finished processing child process. Exiting.. Processed lines: 69500000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 70000000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 70500000 Processed lines: 69000000 Processed lines: 72500000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 70500000 Processed lines: 73000000 Processed lines: 71000000 Processed lines: 72500000 Processed lines: 70000000 Processed lines: 69500000 Processed lines: 69500000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 71000000 Processed lines: 70000000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 70500000 Processed lines: 72000000 Processed lines: 70000000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 71000000 Processed lines: 71500000 Processed lines: 73000000 Processed lines: 70500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 70000000 Processed lines: 70000000 Processed lines: 72500000 Processed lines: 70500000 Processed lines: 71500000 Processed lines: 72500000 Processed lines: 71000000 Finished processing child process. Exiting.. Processed lines: 73000000 Processed lines: 70500000 Processed lines: 71500000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 72500000 Processed lines: 71500000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 71000000 Processed lines: 73000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 71000000 Processed lines: 71500000 Processed lines: 72000000 Processed lines: 73000000 Processed lines: 71000000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 73000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 71500000 Finished processing child process. Exiting.. Processed lines: 71000000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 73000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 72500000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 72000000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 72500000 Finished processing child process. Exiting.. Processed lines: 73000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 73000000 Processed lines: 73000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 73000000 Processed lines: 73000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 73233985 lines in total Total number of methylation call strings processed: 146467970 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1689141290 Total methylated C's in CpG context: 31866013 Total methylated C's in CHG context: 1219594 Total methylated C's in CHH context: 5167236 Total C to T conversions in CpG context: 194609831 Total C to T conversions in CHG context: 309497248 Total C to T conversions in CHH context: 1146781368 C methylated in CpG context: 14.1% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 59M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 59M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 59M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_59M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>64M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >64M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/64M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/64M_R2_val_2.fq.gz" Now reading in Bismark result file 64M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. 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Processed lines: 84500000 Processed lines: 85000000 Processed lines: 89500000 Processed lines: 88500000 Processed lines: 90000000 Processed lines: 84500000 Processed lines: 87500000 Processed lines: 85000000 Processed lines: 86500000 Processed lines: 89500000 Processed lines: 88000000 Processed lines: 87000000 Processed lines: 90000000 Processed lines: 85500000 Processed lines: 86000000 Processed lines: 88500000 Processed lines: 89000000 Processed lines: 84500000 Processed lines: 86000000 Processed lines: 85000000 Processed lines: 87000000 Processed lines: 89500000 Processed lines: 88500000 Processed lines: 90500000 Processed lines: 85000000 Processed lines: 85500000 Processed lines: 86500000 Processed lines: 88000000 Processed lines: 85000000 Processed lines: 90000000 Processed lines: 85500000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 88000000 Processed lines: 85500000 Processed lines: 85000000 Processed lines: 87000000 Processed lines: 90000000 Processed lines: 88500000 Processed lines: 90500000 Processed lines: 87500000 Processed lines: 86000000 Processed lines: 89000000 Processed lines: 89500000 Processed lines: 86500000 Processed lines: 85000000 Processed lines: 85500000 Processed lines: 86500000 Processed lines: 87500000 Processed lines: 91000000 Processed lines: 90000000 Processed lines: 89000000 Processed lines: 87000000 Processed lines: 88500000 Processed lines: 86000000 Processed lines: 85500000 Processed lines: 90500000 Processed lines: 85500000 Processed lines: 89500000 Processed lines: 91000000 Processed lines: 86000000 Processed lines: 88500000 Processed lines: 90500000 Processed lines: 87500000 Processed lines: 85500000 Processed lines: 86000000 Processed lines: 91000000 Processed lines: 89000000 Processed lines: 88000000 Processed lines: 89500000 Processed lines: 90000000 Processed lines: 86500000 Processed lines: 87000000 Processed lines: 91500000 Processed lines: 85500000 Processed lines: 90500000 Processed lines: 86000000 Processed lines: 87000000 Processed lines: 88000000 Processed lines: 89500000 Processed lines: 89000000 Processed lines: 87500000 Processed lines: 86500000 Processed lines: 91000000 Processed lines: 86000000 Processed lines: 91500000 Processed lines: 90000000 Processed lines: 86000000 Processed lines: 86500000 Processed lines: 89000000 Processed lines: 91000000 Processed lines: 91500000 Processed lines: 88000000 Processed lines: 86000000 Processed lines: 89500000 Processed lines: 86500000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 90000000 Processed lines: 87000000 Processed lines: 87500000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 88500000 Processed lines: 86000000 Processed lines: 86500000 Processed lines: 90000000 Processed lines: 87500000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 88000000 Processed lines: 87000000 Processed lines: 86500000 Processed lines: 90500000 Finished processing child process. Exiting.. Processed lines: 86500000 Processed lines: 87000000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 88500000 Processed lines: 90000000 Processed lines: 86500000 Processed lines: 87000000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 87500000 Processed lines: 88000000 Processed lines: 91500000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 87000000 Processed lines: 86500000 Processed lines: 88000000 Processed lines: 90000000 Processed lines: 88500000 Processed lines: 87500000 Processed lines: 91000000 Processed lines: 87000000 Finished processing child process. Exiting.. Processed lines: 87000000 Processed lines: 87500000 Processed lines: 90000000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 87000000 Processed lines: 87500000 Processed lines: 89500000 Finished processing child process. Exiting.. Processed lines: 91000000 Processed lines: 91500000 Processed lines: 88000000 Processed lines: 88500000 Processed lines: 91000000 Processed lines: 89500000 Processed lines: 87500000 Processed lines: 87000000 Processed lines: 88500000 Processed lines: 90500000 Processed lines: 89000000 Processed lines: 91500000 Processed lines: 88000000 Processed lines: 87500000 Processed lines: 87500000 Processed lines: 88000000 Processed lines: 90500000 Processed lines: 91000000 Processed lines: 89500000 Finished processing child process. Exiting.. Processed lines: 88000000 Processed lines: 90000000 Processed lines: 87500000 Processed lines: 91500000 Processed lines: 88500000 Processed lines: 89000000 Finished processing child process. Exiting.. Processed lines: 91500000 Processed lines: 90000000 Processed lines: 91000000 Processed lines: 88000000 Processed lines: 89000000 Processed lines: 87500000 Processed lines: 89500000 Processed lines: 88500000 Processed lines: 88000000 Processed lines: 88000000 Processed lines: 88500000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 90000000 Processed lines: 91500000 Processed lines: 90500000 Processed lines: 88000000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 88500000 Processed lines: 88000000 Processed lines: 90000000 Processed lines: 89000000 Finished processing child process. Exiting.. Processed lines: 88500000 Processed lines: 88500000 Processed lines: 89000000 Processed lines: 91500000 Processed lines: 90500000 Processed lines: 91000000 Processed lines: 89000000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 90000000 Processed lines: 91000000 Processed lines: 90000000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 88500000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 89000000 Processed lines: 89000000 Processed lines: 89500000 Processed lines: 91000000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 89000000 Processed lines: 90000000 Processed lines: 90500000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 90500000 Processed lines: 91000000 Processed lines: 89000000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 89500000 Processed lines: 89500000 Processed lines: 90000000 Processed lines: 91500000 Processed lines: 90000000 Processed lines: 89500000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 91000000 Processed lines: 90000000 Processed lines: 91000000 Processed lines: 91500000 Processed lines: 89500000 Processed lines: 90500000 Finished processing child process. Exiting.. Processed lines: 90000000 Processed lines: 90000000 Processed lines: 90500000 Processed lines: 90500000 Processed lines: 90000000 Processed lines: 91000000 Processed lines: 91500000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 91500000 Processed lines: 91000000 Processed lines: 90000000 Processed lines: 90500000 Processed lines: 90500000 Processed lines: 91000000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 91500000 Finished processing child process. Exiting.. Processed lines: 91000000 Processed lines: 91500000 Processed lines: 90500000 Processed lines: 91000000 Processed lines: 91000000 Processed lines: 91500000 Finished processing child process. Exiting.. Processed lines: 91500000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 91500000 Finished processing child process. Exiting.. Processed lines: 91000000 Processed lines: 91500000 Processed lines: 91500000 Finished processing child process. Exiting.. Processed lines: 91500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 91500000 Now waiting for all child processes to complete Finished processing child process. Exiting.. Merging individual splitting reports into overall report: '64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 91896803 lines in total Total number of methylation call strings processed: 183793606 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2479740246 Total methylated C's in CpG context: 42206303 Total methylated C's in CHG context: 1779052 Total methylated C's in CHH context: 7273814 Total C to T conversions in CpG context: 276397190 Total C to T conversions in CHG context: 438750654 Total C to T conversions in CHH context: 1713333233 C methylated in CpG context: 13.2% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 64M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 64M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 64M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_64M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>6M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >6M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/6M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/6M_R2_val_2.fq.gz" Now reading in Bismark result file 6M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. 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lines: 78500000 Processed lines: 79000000 Processed lines: 77500000 Processed lines: 76500000 Processed lines: 78000000 Processed lines: 76000000 Processed lines: 79500000 Processed lines: 75500000 Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 75500000 Processed lines: 79000000 Processed lines: 76000000 Processed lines: 78500000 Processed lines: 80000000 Processed lines: 75500000 Processed lines: 77500000 Processed lines: 79500000 Processed lines: 76000000 Processed lines: 78000000 Processed lines: 77000000 Processed lines: 76000000 Processed lines: 75500000 Processed lines: 78500000 Processed lines: 80000000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 79000000 Processed lines: 76000000 Processed lines: 79000000 Processed lines: 79500000 Processed lines: 78000000 Processed lines: 77000000 Processed lines: 78500000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 76000000 Processed lines: 77500000 Processed lines: 79500000 Processed lines: 76000000 Processed lines: 76500000 Processed lines: 79000000 Processed lines: 76000000 Processed lines: 80000000 Processed lines: 78000000 Processed lines: 78500000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 77500000 Processed lines: 79000000 Processed lines: 76500000 Processed lines: 76000000 Processed lines: 77000000 Processed lines: 79500000 Processed lines: 76500000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 80000000 Processed lines: 78500000 Processed lines: 77500000 Processed lines: 79000000 Processed lines: 77000000 Processed lines: 80000000 Processed lines: 78000000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 77000000 Processed lines: 79500000 Finished processing child process. Exiting.. Processed lines: 76500000 Processed lines: 78500000 Processed lines: 79000000 Processed lines: 77000000 Finished processing child process. Exiting.. Processed lines: 78000000 Processed lines: 79500000 Processed lines: 77000000 Processed lines: 76500000 Processed lines: 80000000 Processed lines: 77500000 Processed lines: 80000000 Processed lines: 77000000 Processed lines: 79000000 Processed lines: 78000000 Processed lines: 79500000 Processed lines: 77500000 Processed lines: 78500000 Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 77000000 Processed lines: 77500000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 79000000 Processed lines: 77000000 Processed lines: 79500000 Processed lines: 77500000 Processed lines: 78500000 Processed lines: 80000000 Processed lines: 77500000 Processed lines: 77000000 Finished processing child process. Exiting.. Processed lines: 78000000 Processed lines: 79500000 Processed lines: 77500000 Finished processing child process. Exiting.. Processed lines: 78500000 Processed lines: 80000000 Processed lines: 78000000 Processed lines: 79000000 Processed lines: 77500000 Processed lines: 78000000 Processed lines: 77500000 Processed lines: 79500000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 77500000 Processed lines: 79000000 Processed lines: 78000000 Processed lines: 78000000 Processed lines: 77500000 Processed lines: 78500000 Processed lines: 80000000 Processed lines: 78000000 Finished processing child process. Exiting.. Processed lines: 79000000 Processed lines: 78500000 Processed lines: 79500000 Processed lines: 78000000 Processed lines: 78500000 Processed lines: 78000000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 78000000 Processed lines: 79500000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 78000000 Processed lines: 79000000 Finished processing child process. Exiting.. Processed lines: 78500000 Processed lines: 79500000 Processed lines: 79000000 Processed lines: 80000000 Processed lines: 78500000 Processed lines: 79000000 Processed lines: 78500000 Processed lines: 80000000 Processed lines: 78500000 Finished processing child process. Exiting.. Processed lines: 79000000 Processed lines: 79000000 Processed lines: 78500000 Processed lines: 79500000 Processed lines: 79000000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 79000000 Processed lines: 79000000 Finished processing child process. Exiting.. Processed lines: 79000000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 79000000 Processed lines: 80000000 Processed lines: 79500000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 79500000 Finished processing child process. Exiting.. Processed lines: 79500000 Processed lines: 79500000 Processed lines: 80000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 79500000 Processed lines: 80000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 80000000 Finished processing child process. Exiting.. Now waiting for all child processes to complete Finished processing child process. Exiting.. Merging individual splitting reports into overall report: '6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 80219444 lines in total Total number of methylation call strings processed: 160438888 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1995315281 Total methylated C's in CpG context: 36932556 Total methylated C's in CHG context: 1621071 Total methylated C's in CHH context: 7648031 Total C to T conversions in CpG context: 241004510 Total C to T conversions in CHG context: 379429880 Total C to T conversions in CHH context: 1328679233 C methylated in CpG context: 13.3% C methylated in non-CpG context: 0.5% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 6M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 6M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 6M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_6M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>76F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >76F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 76F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/76F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/76F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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75500000 Processed lines: 78000000 Processed lines: 80500000 Processed lines: 80000000 Processed lines: 81000000 Processed lines: 75500000 Processed lines: 76000000 Processed lines: 80000000 Processed lines: 76000000 Processed lines: 81000000 Processed lines: 77500000 Processed lines: 81000000 Processed lines: 79500000 Processed lines: 76000000 Processed lines: 76500000 Processed lines: 79000000 Processed lines: 78500000 Processed lines: 75500000 Processed lines: 80500000 Processed lines: 77000000 Processed lines: 79500000 Processed lines: 77500000 Processed lines: 80000000 Processed lines: 77500000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 79000000 Processed lines: 78000000 Processed lines: 78500000 Processed lines: 76000000 Processed lines: 81000000 Processed lines: 80500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 80500000 Processed lines: 80000000 Processed lines: 76000000 Processed lines: 76500000 Processed lines: 76500000 Processed lines: 78000000 Processed lines: 77000000 Processed lines: 76500000 Processed lines: 79500000 Processed lines: 79000000 Processed lines: 81000000 Processed lines: 76000000 Processed lines: 77500000 Processed lines: 80000000 Processed lines: 78000000 Processed lines: 80500000 Processed lines: 78000000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 79500000 Processed lines: 81500000 Processed lines: 78500000 Processed lines: 79000000 Processed lines: 76500000 Processed lines: 82000000 Processed lines: 81000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 81000000 Processed lines: 80500000 Processed lines: 76500000 Processed lines: 77000000 Processed lines: 78500000 Processed lines: 77000000 Processed lines: 77000000 Processed lines: 77500000 Processed lines: 80000000 Processed lines: 81500000 Processed lines: 79500000 Processed lines: 76500000 Processed lines: 78000000 Processed lines: 80500000 Processed lines: 81000000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 77500000 Processed lines: 80000000 Processed lines: 77500000 Processed lines: 82000000 Processed lines: 79000000 Processed lines: 79500000 Processed lines: 77000000 Processed lines: 82500000 Processed lines: 81500000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 82500000 Processed lines: 81500000 Processed lines: 81000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 77000000 Processed lines: 79000000 Processed lines: 77500000 Processed lines: 77500000 Processed lines: 78000000 Processed lines: 77500000 Processed lines: 80500000 Processed lines: 82000000 Processed lines: 80000000 Processed lines: 81000000 Processed lines: 78500000 Processed lines: 77000000 Processed lines: 81500000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 78000000 Processed lines: 80500000 Processed lines: 82500000 Processed lines: 78000000 Processed lines: 79500000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 77500000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 81500000 Processed lines: 79500000 Processed lines: 77500000 Processed lines: 78000000 Processed lines: 81000000 Processed lines: 82500000 Processed lines: 78500000 Processed lines: 78000000 Processed lines: 78000000 Processed lines: 80500000 Finished processing child process. Exiting.. Processed lines: 81500000 Processed lines: 82000000 Processed lines: 79000000 Processed lines: 77500000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 78500000 Processed lines: 81000000 Processed lines: 78500000 Processed lines: 80000000 Processed lines: 80500000 Processed lines: 82500000 Processed lines: 78000000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 81500000 Processed lines: 78000000 Processed lines: 79000000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 78500000 Processed lines: 81000000 Processed lines: 82000000 Processed lines: 82500000 Processed lines: 79500000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 78000000 Processed lines: 80000000 Processed lines: 81500000 Processed lines: 79000000 Processed lines: 81000000 Processed lines: 80500000 Processed lines: 79000000 Processed lines: 78500000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 80500000 Processed lines: 82000000 Processed lines: 78500000 Processed lines: 79500000 Processed lines: 79000000 Processed lines: 81500000 Processed lines: 79000000 Processed lines: 79000000 Processed lines: 82500000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 80500000 Processed lines: 78500000 Processed lines: 80500000 Processed lines: 82000000 Processed lines: 79500000 Processed lines: 81500000 Processed lines: 81000000 Processed lines: 79500000 Processed lines: 79000000 Processed lines: 82500000 Processed lines: 81000000 Processed lines: 79000000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 82000000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 79500000 Processed lines: 80500000 Processed lines: 81000000 Processed lines: 79000000 Processed lines: 81000000 Processed lines: 82500000 Processed lines: 80000000 Processed lines: 82000000 Processed lines: 81500000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 79500000 Processed lines: 81500000 Processed lines: 82500000 Processed lines: 80500000 Processed lines: 79500000 Processed lines: 80000000 Processed lines: 80000000 Processed lines: 80000000 Finished processing child process. Exiting.. Processed lines: 81000000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 79500000 Processed lines: 80500000 Processed lines: 82500000 Processed lines: 82000000 Processed lines: 80500000 Finished processing child process. Exiting.. Processed lines: 80000000 Processed lines: 82000000 Processed lines: 80000000 Processed lines: 81000000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 80500000 Processed lines: 81500000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 80000000 Processed lines: 81000000 Processed lines: 82500000 Processed lines: 81000000 Finished processing child process. Exiting.. Processed lines: 80500000 Processed lines: 82500000 Processed lines: 81500000 Finished processing child process. Exiting.. Processed lines: 80500000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 81000000 Processed lines: 82000000 Processed lines: 82500000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 80500000 Finished processing child process. Exiting.. Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81000000 Processed lines: 82000000 Processed lines: 81000000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 81000000 Processed lines: 82000000 Processed lines: 82000000 Processed lines: 81500000 Processed lines: 81500000 Processed lines: 82000000 Processed lines: 82500000 Processed lines: 82000000 Processed lines: 82000000 Finished processing child process. Exiting.. Processed lines: 81500000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 82000000 Processed lines: 82000000 Processed lines: 82500000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 82500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 82000000 Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 82500000 Finished processing child process. Exiting.. Processed lines: 82500000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 82560622 lines in total Total number of methylation call strings processed: 165121244 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2243661587 Total methylated C's in CpG context: 34487480 Total methylated C's in CHG context: 1538367 Total methylated C's in CHH context: 6745430 Total C to T conversions in CpG context: 252523035 Total C to T conversions in CHG context: 397100054 Total C to T conversions in CHH context: 1551267221 C methylated in CpG context: 12.0% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 76F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 76F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 76F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_76F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>77F_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >77F_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 77F_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/77F_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/77F_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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lines: 88500000 Processed lines: 93000000 Processed lines: 90000000 Processed lines: 93500000 Processed lines: 94500000 Processed lines: 89000000 Processed lines: 94500000 Processed lines: 93500000 Processed lines: 88000000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 89500000 Processed lines: 92000000 Processed lines: 91000000 Processed lines: 90000000 Processed lines: 88500000 Processed lines: 94000000 Processed lines: 92500000 Processed lines: 89000000 Processed lines: 91500000 Processed lines: 88500000 Processed lines: 88500000 Processed lines: 91000000 Processed lines: 93000000 Processed lines: 94500000 Processed lines: 92000000 Processed lines: 93000000 Processed lines: 90000000 Processed lines: 93500000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 94000000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 94000000 Processed lines: 89500000 Processed lines: 91000000 Processed lines: 89500000 Processed lines: 92500000 Processed lines: 88500000 Processed lines: 90000000 Processed lines: 91500000 Processed lines: 90500000 Processed lines: 89000000 Processed lines: 94500000 Processed lines: 93000000 Processed lines: 89500000 Processed lines: 92000000 Processed lines: 89000000 Processed lines: 91500000 Processed lines: 89000000 Processed lines: 95000000 Processed lines: 93500000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 94000000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 94500000 Processed lines: 89500000 Processed lines: 91000000 Finished processing child process. Exiting.. Processed lines: 94500000 Processed lines: 91500000 Processed lines: 93000000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 89000000 Processed lines: 90500000 Processed lines: 92000000 Processed lines: 93500000 Processed lines: 95000000 Processed lines: 91000000 Processed lines: 89500000 Processed lines: 90000000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 92000000 Processed lines: 94000000 Processed lines: 89500000 Processed lines: 89500000 Processed lines: 93000000 Processed lines: 94000000 Processed lines: 94500000 Processed lines: 95000000 Processed lines: 91000000 Processed lines: 90000000 Processed lines: 91500000 Processed lines: 95000000 Processed lines: 93500000 Processed lines: 92000000 Processed lines: 90500000 Processed lines: 90500000 Processed lines: 89500000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 91000000 Processed lines: 94000000 Processed lines: 91500000 Processed lines: 90000000 Processed lines: 90500000 Processed lines: 93000000 Processed lines: 94500000 Processed lines: 92500000 Processed lines: 93500000 Processed lines: 94500000 Processed lines: 90000000 Processed lines: 90000000 Processed lines: 95000000 Finished processing child process. Exiting.. Processed lines: 91500000 Finished processing child process. Exiting.. Processed lines: 90500000 Processed lines: 92000000 Processed lines: 94000000 Processed lines: 92500000 Processed lines: 91000000 Processed lines: 91000000 Processed lines: 93000000 Processed lines: 90000000 Processed lines: 91500000 Processed lines: 94500000 Processed lines: 92000000 Processed lines: 90500000 Processed lines: 91000000 Processed lines: 93500000 Processed lines: 95000000 Processed lines: 93000000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 95000000 Processed lines: 90500000 Processed lines: 90500000 Processed lines: 92000000 Processed lines: 92500000 Processed lines: 91000000 Processed lines: 94500000 Processed lines: 93000000 Processed lines: 93500000 Processed lines: 91500000 Processed lines: 91500000 Processed lines: 92000000 Processed lines: 90500000 Processed lines: 95000000 Processed lines: 92500000 Processed lines: 91000000 Processed lines: 94000000 Processed lines: 91500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 94500000 Processed lines: 93500000 Processed lines: 91000000 Processed lines: 91000000 Processed lines: 92500000 Processed lines: 93000000 Processed lines: 91500000 Processed lines: 95000000 Processed lines: 93500000 Finished processing child process. Exiting.. Processed lines: 92000000 Processed lines: 94000000 Processed lines: 92000000 Processed lines: 92500000 Processed lines: 91000000 Processed lines: 93000000 Processed lines: 91500000 Processed lines: 94500000 Processed lines: 92000000 Processed lines: 95000000 Processed lines: 94000000 Processed lines: 91500000 Processed lines: 91500000 Processed lines: 93000000 Processed lines: 93500000 Processed lines: 92000000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 94500000 Processed lines: 92500000 Processed lines: 93000000 Processed lines: 92500000 Processed lines: 91500000 Processed lines: 93500000 Processed lines: 95000000 Processed lines: 92000000 Finished processing child process. Exiting.. Processed lines: 92500000 Processed lines: 94500000 Processed lines: 92000000 Processed lines: 92000000 Processed lines: 93500000 Processed lines: 94000000 Processed lines: 92500000 Processed lines: 94500000 Processed lines: 95000000 Processed lines: 93500000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 92000000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 93000000 Processed lines: 92500000 Processed lines: 95000000 Processed lines: 92500000 Processed lines: 92500000 Processed lines: 94500000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 95000000 Finished processing child process. Exiting.. Processed lines: 94000000 Processed lines: 93500000 Processed lines: 93500000 Processed lines: 92500000 Processed lines: 94500000 Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 93000000 Processed lines: 95000000 Processed lines: 93500000 Processed lines: 94500000 Finished processing child process. Exiting.. Processed lines: 94500000 Processed lines: 94000000 Processed lines: 94000000 Processed lines: 93000000 Processed lines: 95000000 Processed lines: 94000000 Processed lines: 93500000 Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 93500000 Processed lines: 94000000 Processed lines: 95000000 Processed lines: 95000000 Processed lines: 94500000 Processed lines: 94500000 Finished processing child process. Exiting.. Processed lines: 93500000 Processed lines: 94000000 Processed lines: 94500000 Processed lines: 94000000 Processed lines: 94000000 Finished processing child process. Exiting.. Processed lines: 94500000 Finished processing child process. Exiting.. Processed lines: 95000000 Processed lines: 95000000 Processed lines: 94000000 Processed lines: 94500000 Processed lines: 95000000 Processed lines: 94500000 Processed lines: 94500000 Processed lines: 95000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 94500000 Finished processing child process. Exiting.. Processed lines: 95000000 Processed lines: 95000000 Processed lines: 95000000 Finished processing child process. Exiting.. Processed lines: 95000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Merging individual splitting reports into overall report: '77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 95462715 lines in total Total number of methylation call strings processed: 190925430 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2499600963 Total methylated C's in CpG context: 36760446 Total methylated C's in CHG context: 1702958 Total methylated C's in CHH context: 7092183 Total C to T conversions in CpG context: 283258341 Total C to T conversions in CHG context: 441615989 Total C to T conversions in CHH context: 1729171046 C methylated in CpG context: 11.5% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 77F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 77F_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 77F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_77F_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>7M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >7M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 7M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/7M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/7M_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 1000000 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68500000 Processed lines: 69500000 Processed lines: 74000000 Processed lines: 69500000 Processed lines: 71500000 Processed lines: 70000000 Processed lines: 71000000 Processed lines: 73500000 Processed lines: 70500000 Processed lines: 73000000 Processed lines: 72000000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 72500000 Processed lines: 74500000 Processed lines: 74000000 Processed lines: 72500000 Processed lines: 70500000 Processed lines: 73000000 Processed lines: 71500000 Processed lines: 72000000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 71000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 74500000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 72000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 74000000 Processed lines: 70500000 Processed lines: 71500000 Processed lines: 73500000 Processed lines: 74000000 Processed lines: 71000000 Processed lines: 72500000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 73000000 Processed lines: 75000000 Processed lines: 73000000 Processed lines: 71000000 Processed lines: 73500000 Processed lines: 72000000 Processed lines: 72500000 Processed lines: 75000000 Processed lines: 71500000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 69500000 Processed lines: 72500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 70500000 Processed lines: 74500000 Processed lines: 70500000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 74500000 Processed lines: 74000000 Processed lines: 73000000 Processed lines: 71500000 Processed lines: 75000000 Processed lines: 74500000 Processed lines: 75500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 71500000 Processed lines: 74000000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 75500000 Processed lines: 72000000 Processed lines: 73000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 75000000 Processed lines: 70000000 Processed lines: 70500000 Processed lines: 70000000 Processed lines: 70500000 Processed lines: 70000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71500000 Processed lines: 75000000 Processed lines: 72500000 Processed lines: 74500000 Processed lines: 73500000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 75000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 72000000 Processed lines: 74500000 Finished processing child process. Exiting.. Processed lines: 73500000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 73500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 75000000 Processed lines: 73000000 Processed lines: 74000000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 75500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 72500000 Processed lines: 74000000 Processed lines: 73500000 Finished processing child process. Exiting.. Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 74000000 Processed lines: 71000000 Processed lines: 75500000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 72500000 Processed lines: 72000000 Processed lines: 73500000 Processed lines: 74500000 Processed lines: 71500000 Processed lines: 71500000 Finished processing child process. Exiting.. Processed lines: 73000000 Processed lines: 75000000 Processed lines: 75500000 Processed lines: 75000000 Processed lines: 74500000 Processed lines: 73000000 Processed lines: 74000000 Processed lines: 73500000 Finished processing child process. Exiting.. Processed lines: 74500000 Processed lines: 74000000 Processed lines: 73000000 Processed lines: 71500000 Processed lines: 75000000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 73500000 Processed lines: 75500000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 75000000 Processed lines: 73500000 Processed lines: 74500000 Processed lines: 74000000 Processed lines: 75000000 Processed lines: 74500000 Processed lines: 75500000 Processed lines: 73500000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 74000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 75500000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 75500000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 74000000 Processed lines: 74500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 73000000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 74500000 Processed lines: 75000000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 73500000 Processed lines: 73500000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 75500000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 75500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 73500000 Processed lines: 74000000 Processed lines: 74000000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 75500000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 75000000 Processed lines: 74000000 Processed lines: 74000000 Processed lines: 74000000 Finished processing child process. Exiting.. Processed lines: 74500000 Processed lines: 74500000 Finished processing child process. Exiting.. Processed lines: 75500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 74500000 Processed lines: 75500000 Processed lines: 74500000 Processed lines: 75000000 Processed lines: 75000000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 75000000 Processed lines: 75000000 Finished processing child process. Exiting.. Processed lines: 75000000 Processed lines: 75000000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Processed lines: 75500000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Now waiting for all child processes to complete Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Merging individual splitting reports into overall report: '7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 75968903 lines in total Total number of methylation call strings processed: 151937806 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2148117321 Total methylated C's in CpG context: 38122812 Total methylated C's in CHG context: 1485354 Total methylated C's in CHH context: 6163716 Total C to T conversions in CpG context: 236284094 Total C to T conversions in CHG context: 381726093 Total C to T conversions in CHH context: 1484335252 C methylated in CpG context: 13.9% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 7M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 7M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 7M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_7M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Checking file >>9M_R1_val_1_bismark_bt2_pe.deduplicated.bam<< for signs of file truncation... Now testing Bismark result file >9M_R1_val_1_bismark_bt2_pe.deduplicated.bam< for positional sorting (which would be bad...) ...passed! Writing result file containing methylation information for C in CpG context to CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing result file containing methylation information for C in any other context to Non_CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Now reading in Bismark result file 9M_R1_val_1_bismark_bt2_pe.deduplicated.bam Warning: unable to close filehandle IN properly. skipping SAM header line: @HD VN:1.0 SO:unsorted skipping SAM header line: @SQ SN:NC_035780.1 LN:65668440 skipping SAM header line: @SQ SN:NC_035781.1 LN:61752955 skipping SAM header line: @SQ SN:NC_035782.1 LN:77061148 skipping SAM header line: @SQ SN:NC_035783.1 LN:59691872 skipping SAM header line: @SQ SN:NC_035784.1 LN:98698416 skipping SAM header line: @SQ SN:NC_035785.1 LN:51258098 skipping SAM header line: @SQ SN:NC_035786.1 LN:57830854 skipping SAM header line: @SQ SN:NC_035787.1 LN:75944018 skipping SAM header line: @SQ SN:NC_035788.1 LN:104168038 skipping SAM header line: @SQ SN:NC_035789.1 LN:32650045 skipping SAM header line: @SQ SN:NC_007175.2 LN:17244 skipping SAM header line: @PG ID:Bismark VN:v0.22.3 CL:"bismark --path_to_bowtie /gscratch/srlab/programs/bowtie2-2.3.4.1-linux-x86_64/ -genome /gscratch/srlab/sr320/data/Cvirg-genome/ -p 4 -score_min L,0,-0.6 --non_directional -1 /gscratch/srlab/sr320/data/cvirg/9M_R1_val_1.fq.gz -2 /gscratch/srlab/sr320/data/cvirg/9M_R2_val_2.fq.gz" Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 500000 Processed lines: 1000000 Processed lines: 1000000 Processed lines: 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Processed lines: 69500000 Processed lines: 71000000 Processed lines: 72500000 Processed lines: 68000000 Processed lines: 68000000 Processed lines: 72000000 Processed lines: 68000000 Processed lines: 70000000 Processed lines: 69000000 Processed lines: 70500000 Processed lines: 71000000 Processed lines: 72000000 Processed lines: 71500000 Processed lines: 70500000 Processed lines: 67500000 Processed lines: 68500000 Processed lines: 69500000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 71000000 Processed lines: 72500000 Processed lines: 72000000 Processed lines: 70500000 Processed lines: 71000000 Processed lines: 68000000 Processed lines: 68000000 Processed lines: 70000000 Processed lines: 68000000 Processed lines: 71500000 Processed lines: 69500000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 70500000 Processed lines: 69500000 Processed lines: 71500000 Processed lines: 71000000 Processed lines: 72500000 Processed lines: 72000000 Processed lines: 71000000 Processed lines: 70000000 Finished processing child process. Exiting.. Processed lines: 68000000 Processed lines: 71500000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 71500000 Processed lines: 72000000 Processed lines: 70500000 Processed lines: 70000000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 68500000 Processed lines: 73000000 Processed lines: 71000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 70000000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 73000000 Processed lines: 71500000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 70500000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 68500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 73000000 Processed lines: 71500000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 70500000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 69000000 Processed lines: 71500000 Processed lines: 70500000 Processed lines: 72500000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 69500000 Processed lines: 69500000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 71000000 Processed lines: 72500000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 69000000 Processed lines: 72000000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 71500000 Processed lines: 71000000 Finished processing child process. Exiting.. Processed lines: 69500000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 69500000 Processed lines: 73000000 Processed lines: 71000000 Processed lines: 72500000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 72500000 Finished processing child process. Exiting.. Processed lines: 71500000 Processed lines: 73000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 69500000 Processed lines: 72000000 Processed lines: 71500000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 70000000 Processed lines: 71500000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 73000000 Processed lines: 72000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 70500000 Finished processing child process. Exiting.. Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 72000000 Finished processing child process. Exiting.. Processed lines: 73000000 Processed lines: 70000000 Processed lines: 70500000 Processed lines: 70500000 Processed lines: 72000000 Processed lines: 70500000 Finished processing child process. Exiting.. Processed lines: 72500000 Finished processing child process. Exiting.. Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 71000000 Processed lines: 70500000 Processed lines: 71000000 Processed lines: 71000000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 72000000 Processed lines: 72000000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 72000000 Processed lines: 73000000 Finished processing child process. Exiting.. Processed lines: 71500000 Processed lines: 71500000 Processed lines: 71500000 Processed lines: 73000000 Processed lines: 71000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 72000000 Processed lines: 71500000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72500000 Processed lines: 72000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 72500000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Processed lines: 73000000 Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Finished processing child process. Exiting.. Processed lines: 73000000 Now waiting for all child processes to complete Merging individual splitting reports into overall report: '9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt' Merging from these individual files: 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28 Processed 73301023 lines in total Total number of methylation call strings processed: 146602046 Final Cytosine Methylation Report ================================= Total number of C's analysed: 2060013095 Total methylated C's in CpG context: 34898355 Total methylated C's in CHG context: 1383254 Total methylated C's in CHH context: 5644769 Total C to T conversions in CpG context: 232890147 Total C to T conversions in CHG context: 367770942 Total C to T conversions in CHH context: 1417425628 C methylated in CpG context: 13.0% C methylated in non-CpG context: 0.4% Merging individual M-bias reports into overall M-bias statistics from these 28 individual files: 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.1.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.2.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.3.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.4.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.5.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.6.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.7.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.8.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.9.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.10.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.11.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.12.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.13.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.14.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.15.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.16.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.17.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.18.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.19.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.20.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.21.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.22.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.23.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.24.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.25.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.26.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.27.mbias 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt.28.mbias Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Determining maximum read lengths for M-Bias plots Maximum read length of Read 1: 136 Maximum read length of Read 2: 136 Perl module GD::Graph::lines is not installed, skipping drawing M-bias plots (only writing out M-bias plot table) Deleting unused files ... CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Non_CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt contains data -> kept Using these input files: CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt Non_CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt Summary of parameters for bismark2bedGraph conversion: ====================================================== bedGraph output: 9M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz output directory: >< remove whitespaces: no CX context: no (CpG context only, default) No-header selected: no Sorting method: Unix sort-based (smaller memory footprint, but slower) Sort buffer size: 75% Coverage threshold: 1 ============================================================================= Methylation information will now be written into a bedGraph and coverage file ============================================================================= Using the following files as Input: /gscratch/scrubbed/sr320/071521-cvBS/CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt Writing bedGraph to file: 9M_R1_val_1_bismark_bt2_pe.deduplicated.bedGraph.gz Also writing out a coverage file including counts methylated and unmethylated residues to file: 9M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Now writing methylation information for file >>CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt<< to individual files for each chromosome Finished writing out individual chromosome files for CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt Collecting temporary chromosome file information... Processing the following input file(s): CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035785.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035788.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035784.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035780.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035781.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035782.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035786.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035787.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035783.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_035789.1.methXtractor.temp Sorting input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp by positions (using -S of 75%) Successfully deleted the temporary input file CpG_context_9M_R1_val_1_bismark_bt2_pe.deduplicated.txt.chrNC_007175.2.methXtractor.temp Finished BedGraph conversion ... Found 26 alignment reports in current directory. Now trying to figure out whether there are corresponding optional reports Writing Bismark HTML report to >> 12M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 12M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 12M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 12M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 12M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 12M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 12M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 12M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 13M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 13M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 13M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 13M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 13M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 13M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 13M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 13M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 16F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 16F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 16F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 16F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 16F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 16F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 16F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 16F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 19F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 19F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 19F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 19F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 19F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 19F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 19F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 19F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 22F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 22F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 22F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 22F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 22F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 22F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 22F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 22F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 23M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 23M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 23M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 23M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 23M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 23M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 23M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 23M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 29F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 29F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 29F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 29F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 29F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 29F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 29F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 29F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 31M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 31M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 31M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 31M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 31M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 31M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 31M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 31M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 35F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 35F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 35F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 35F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 35F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 35F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 35F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 35F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 36F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 36F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 36F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 36F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 36F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 36F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 36F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 36F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 39F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 39F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 39F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 39F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 39F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 39F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 39F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 39F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 3F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 3F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 3F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 3F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 3F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 3F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 3F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 3F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 41F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 41F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 41F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 41F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 41F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 41F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 41F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 41F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 44F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 44F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 44F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 44F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 44F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 44F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 44F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 44F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 48M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 48M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 48M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 48M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 48M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 48M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 48M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 48M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 50F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 50F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 50F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 50F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 50F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 50F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 50F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 50F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 52F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 52F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 52F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 52F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 52F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 52F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 52F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 52F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 53F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 53F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 53F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 53F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 53F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 53F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 53F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 53F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 54F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 54F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 54F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 54F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 54F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 54F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 54F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 54F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 59M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 59M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 59M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 59M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 59M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 59M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 59M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 59M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 64M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 64M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 64M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 64M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 64M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 64M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 64M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 64M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 6M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 6M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 6M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 6M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 6M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 6M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 6M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 6M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 76F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 76F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 76F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 76F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 76F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 76F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 76F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 76F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 77F_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 77F_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 77F_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 77F_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 77F_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 77F_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 77F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 77F_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 7M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 7M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 7M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 7M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 7M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 7M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 7M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 7M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== Writing Bismark HTML report to >> 9M_R1_val_1_bismark_bt2_PE_report.html << ============================================================================================================== Using the following alignment report: > 9M_R1_val_1_bismark_bt2_PE_report.txt < Processing alignment report 9M_R1_val_1_bismark_bt2_PE_report.txt ... Complete Using the following deduplication report: > 9M_R1_val_1_bismark_bt2_pe.deduplication_report.txt < Processing deduplication report 9M_R1_val_1_bismark_bt2_pe.deduplication_report.txt ... Complete Using the following splitting report: > 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt < Processing splitting report 9M_R1_val_1_bismark_bt2_pe.deduplicated_splitting_report.txt ... Complete Using the following M-bias report: > 9M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt < Processing M-bias report 9M_R1_val_1_bismark_bt2_pe.deduplicated.M-bias.txt ... Complete No nucleotide coverage report file specified, skipping this step ============================================================================================================== No Bismark/Bowtie2 single-end BAM files detected Found Bismark/Bowtie2 paired-end files No Bismark/HISAT2 single-end BAM files detected No Bismark/HISAT2 paired-end BAM files detected Generating Bismark summary report from 26 Bismark BAM file(s)... >> Reading from Bismark report: 12M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 13M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 16F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 19F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 22F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 23M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 29F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 31M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 35F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 36F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 39F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 3F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 41F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 44F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 48M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 50F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 52F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 53F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 54F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 59M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 64M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 6M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 76F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 77F_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 7M_R1_val_1_bismark_bt2_PE_report.txt >> Reading from Bismark report: 9M_R1_val_1_bismark_bt2_PE_report.txt Wrote Bismark project summary to >> bismark_summary_report.html << [INFO ] multiqc : This is MultiQC v1.8 [INFO ] multiqc : Template : default [INFO ] multiqc : Searching : /gscratch/scrubbed/sr320/071521-cvBS [INFO ] bismark : Found 26 bismark alignment reports [INFO ] bismark : Found 26 bismark dedup reports [INFO ] bismark : Found 26 bismark methextract reports [INFO ] fastqc : Found 52 reports [INFO ] multiqc : Compressing plot data [INFO ] multiqc : Report : multiqc_report.html [INFO ] multiqc : Data : multiqc_data [INFO ] multiqc : MultiQC complete [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 140 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 140 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 56 files and 28 in-memory blocks... [bam_sort_core] merging from 196 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 112 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... [bam_sort_core] merging from 84 files and 28 in-memory blocks... Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 12M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 12M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035787.1 Writing cytosine report for chromosome NC_035787.1 (stored 2283366 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2250237 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2021624 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1009987 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3347376 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3096730 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2459811 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2017251 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1716154 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1478817 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 947 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>12M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 12M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 13M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 13M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035780.1 Writing cytosine report for chromosome NC_035780.1 (stored 2267880 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2465296 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1582559 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3345680 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3062465 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2011533 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2016963 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1402020 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1024434 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2312370 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 957 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>13M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 13M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 16F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 16F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035787.1 Writing cytosine report for chromosome NC_035787.1 (stored 2320628 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3111818 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2464396 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2269966 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2018030 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3337005 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1486010 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1000933 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1731853 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2007606 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1342 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>16F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 16F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 19F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 19F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035784.1 Writing cytosine report for chromosome NC_035784.1 (stored 3358514 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2022168 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3086512 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2463550 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2295853 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2019939 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1011381 different covered positions) Writing cytosine report for chromosome NC_007175.2 (stored 1757 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1451946 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2264695 different covered positions) Writing cytosine report for last chromosome NC_035786.1 (stored 1693138 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>19F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 19F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 22F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 22F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035786.1 Writing cytosine report for chromosome NC_035786.1 (stored 1654942 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2221195 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3270542 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2418823 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 968796 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2930692 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1982334 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1404609 different covered positions) Writing cytosine report for chromosome NC_007175.2 (stored 1621 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1958446 different covered positions) Writing cytosine report for last chromosome NC_035780.1 (stored 2211220 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>22F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 22F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 23M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 23M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035780.1 Writing cytosine report for chromosome NC_035780.1 (stored 2262295 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3068137 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1712136 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2018067 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3382945 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2034276 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2301547 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2470140 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1482397 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1041473 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 936 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>23M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 23M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 29F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 29F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035784.1 Writing cytosine report for chromosome NC_035784.1 (stored 3318709 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2239860 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3045922 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2281221 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1443303 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2420314 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1990432 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1990712 different covered positions) Writing cytosine report for chromosome NC_007175.2 (stored 1382 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1674522 different covered positions) Writing cytosine report for last chromosome NC_035789.1 (stored 1006870 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>29F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 29F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 31M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 31M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035786.1 Writing cytosine report for chromosome NC_035786.1 (stored 1591087 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2332886 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2903195 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1972932 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3471369 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1936779 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2260712 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1392899 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 938085 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2129790 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 903 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>31M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 31M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 35F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 35F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035785.1 Writing cytosine report for chromosome NC_035785.1 (stored 1426437 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1700145 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2446349 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3340828 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2256165 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3028099 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1007731 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2281384 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2016474 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1996555 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1757 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>35F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 35F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 36F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 36F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035780.1 Writing cytosine report for chromosome NC_035780.1 (stored 2231041 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1709564 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2006036 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2002621 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3056174 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1446714 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2438237 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3313601 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2268610 different covered positions) Writing cytosine report for chromosome NC_007175.2 (stored 1681 different covered positions) Writing cytosine report for last chromosome NC_035789.1 (stored 1028710 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>36F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 36F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 39F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 39F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035786.1 Writing cytosine report for chromosome NC_035786.1 (stored 1665743 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1972719 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2223253 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1006277 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2996548 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3331499 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2231429 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2421543 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1983347 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1431789 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1784 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>39F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 39F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 3F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 3F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035787.1 Writing cytosine report for chromosome NC_035787.1 (stored 2266858 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2398132 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1987641 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1996021 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3365847 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1529082 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2218530 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1690836 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3096603 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1096579 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1630 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>3F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 3F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 41F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 41F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035783.1 Writing cytosine report for chromosome NC_035783.1 (stored 1922861 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2154454 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1372409 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2133796 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1543872 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3228312 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1940753 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2893078 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2320502 different covered positions) Writing cytosine report for chromosome NC_007175.2 (stored 1390 different covered positions) Writing cytosine report for last chromosome NC_035789.1 (stored 936838 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>41F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 41F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 44F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 44F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035784.1 Writing cytosine report for chromosome NC_035784.1 (stored 3521014 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2600554 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1105692 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3295447 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1838961 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2389880 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2132145 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2454438 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1554433 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2117041 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1565 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>44F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 44F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 48M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 48M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035782.1 Writing cytosine report for chromosome NC_035782.1 (stored 2409593 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2987846 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2207955 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1663658 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1970760 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2213204 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1976220 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3271605 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 977735 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1424019 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 996 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>48M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 48M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 50F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 50F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035784.1 Writing cytosine report for chromosome NC_035784.1 (stored 3319024 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2445190 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1028655 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2299695 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2001538 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1724895 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3079333 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1459903 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2248325 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2012085 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1593 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>50F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 50F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 52F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 52F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035782.1 Writing cytosine report for chromosome NC_035782.1 (stored 2465745 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2326505 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2025700 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3375100 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3111708 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2022914 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1024020 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1714239 different covered positions) Writing cytosine report for chromosome NC_007175.2 (stored 1396 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2283206 different covered positions) Writing cytosine report for last chromosome NC_035785.1 (stored 1482645 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>52F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 52F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 53F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 53F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035785.1 Writing cytosine report for chromosome NC_035785.1 (stored 1469356 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1697374 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2286229 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3357023 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2266137 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2023298 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2468371 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3087402 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2020729 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1020920 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1668 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>53F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 53F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 54F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 54F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035782.1 Writing cytosine report for chromosome NC_035782.1 (stored 2476787 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3381733 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 2024609 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2263925 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 2023430 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2317586 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1700093 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1476619 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3047017 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1026291 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1501 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>54F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 54F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 59M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 59M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035785.1 Writing cytosine report for chromosome NC_035785.1 (stored 1342825 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2146461 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1913600 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1901658 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3141612 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2834162 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2142337 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2306070 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 910124 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1538012 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 905 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>59M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 59M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 64M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 64M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035782.1 Writing cytosine report for chromosome NC_035782.1 (stored 2408459 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2211476 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1984778 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2224056 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3298882 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1987920 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1005421 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1424250 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2975513 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1677372 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 955 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>64M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 64M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 6M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 6M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035788.1 Writing cytosine report for chromosome NC_035788.1 (stored 2948362 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1938101 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2340704 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2169462 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1930489 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2179033 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3248885 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1380706 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 967419 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1646998 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 990 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>6M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 6M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 76F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 76F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035789.1 Writing cytosine report for chromosome NC_035789.1 (stored 960357 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1988111 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2252044 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2158309 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1978663 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2428062 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3307622 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1675532 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 3003556 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1409504 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1581 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>76F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 76F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 77F_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 77F_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035787.1 Writing cytosine report for chromosome NC_035787.1 (stored 2261644 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1997367 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 1011331 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2226752 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1986246 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1435977 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3287286 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2995144 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2417161 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1690288 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 1495 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>77F_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 77F_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 7M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 7M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035782.1 Writing cytosine report for chromosome NC_035782.1 (stored 2415057 different covered positions) Writing cytosine report for chromosome NC_035785.1 (stored 1421164 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1990315 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3332211 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2950399 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2250806 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 995649 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2221867 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1994301 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1650744 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 980 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>7M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 7M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq! Summary of parameters for genome-wide cytosine report: ============================================================================== Coverage infile: 9M_R1_val_1_bismark_bt2_pe.deduplicated.bismark.cov.gz Output directory: >< Parent directory: >/gscratch/scrubbed/sr320/071521-cvBS/< Genome directory: >/gscratch/srlab/sr320/data/Cvirg-genome/< Coverage threshold 0 (all positions will be reported, default) CX context: no (CpG context only, default) Pooling CpG top/bottom strand evidence: yes Genome coordinates used: 0-based (user specified) GZIP compression: no Split by chromosome: no Now reading in and storing sequence information of the genome specified in: /gscratch/srlab/sr320/data/Cvirg-genome/ chr NC_035780.1 (65668440 bp) chr NC_035781.1 (61752955 bp) chr NC_035782.1 (77061148 bp) chr NC_035783.1 (59691872 bp) chr NC_035784.1 (98698416 bp) chr NC_035785.1 (51258098 bp) chr NC_035786.1 (57830854 bp) chr NC_035787.1 (75944018 bp) chr NC_035788.1 (104168038 bp) chr NC_035789.1 (32650045 bp) chr NC_007175.2 (17244 bp) Stored sequence information of 11 chromosomes/scaffolds in total ============================================================================== Methylation information will now be written into a genome-wide cytosine report ============================================================================== >>> Writing genome-wide cytosine report to: 9M_R1_val_1.CpG_report.txt <<< Storing all covered cytosine positions for chromosome: NC_035785.1 Writing cytosine report for chromosome NC_035785.1 (stored 1376004 different covered positions) Writing cytosine report for chromosome NC_035788.1 (stored 2934386 different covered positions) Writing cytosine report for chromosome NC_035784.1 (stored 3323539 different covered positions) Writing cytosine report for chromosome NC_035780.1 (stored 2169193 different covered positions) Writing cytosine report for chromosome NC_035781.1 (stored 1966949 different covered positions) Writing cytosine report for chromosome NC_035782.1 (stored 2404901 different covered positions) Writing cytosine report for chromosome NC_035786.1 (stored 1664000 different covered positions) Writing cytosine report for chromosome NC_035787.1 (stored 2212185 different covered positions) Writing cytosine report for chromosome NC_035783.1 (stored 1963993 different covered positions) Writing cytosine report for chromosome NC_035789.1 (stored 877903 different covered positions) Writing cytosine report for last chromosome NC_007175.2 (stored 908 different covered positions) Finished writing out cytosine report for covered chromosomes (processed 11 chromosomes/scaffolds in total) Now processing chromosomes that were not covered by any methylation calls in the coverage file... All chromosomes in the genome were covered by at least some reads. coverage2cytosine processing complete. Now merging top and bottom strand CpGs into a single CG dinucleotide entity (reading from file >>9M_R1_val_1.CpG_report.txt<<, in output directory '') >>> Writing a new coverage file with top and bottom strand CpG methylation evidence merged to 9M_R1_val_1.CpG_report.merged_CpG_evidence.cov <<< CpG merging complete. Good luck finding DMRs with bsseq!